PMID- 34841692
OWN - NLM
STAT- MEDLINE
DCOM- 20220331
LR  - 20220401
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Linking)
VI  - 66
IP  - 2
DP  - 2022 Jan
TI  - Cyanidin-3-O-Glucoside Supplementation Ameliorates Metabolic Insulin Resistance 
      via Restoration of Nitric Oxide-Mediated Endothelial Insulin Transport.
PG  - e2100742
LID - 10.1002/mnfr.202100742 [doi]
AB  - SCOPE: Anthocyanin cyanidin-3-O-glucoside (Cy3G) possesses a great potential in 
      prevention of diabetes and its vascular complications while the underlying 
      mechanisms are still far from clear. Accumulating evidence suggests that 
      endothelial insulin transport plays a critical role in regulating metabolic 
      insulin sensitivity. Whether Cy3G can modulate metabolic insulin resistance via 
      regulating endothelial insulin transport is not reported yet. METHODS AND 
      RESULTS: Palmitic acid (PA)-treated mouse aortic endothelial cells (MAECs) model 
      and high-fat diet (HFD) fed mice model are used. Compared with HFD mice, Cy3G 
      supplementation decrease exogenous insulin content in skeletal muscle and 
      ameliorate metabolic insulin resistance. In culture, Cy3G can directly ameliorate 
      PA-induced impairment on FITC-insulin uptake in MAECs. Mechanistically, Cy3G can 
      effectively decrease inflammatory cytokines and toll-like receptor 4 
      (TLR4)/nuclear factor-kappa-B inhibitor alpha (IkappaBalpha) activation, and restore the 
      attenuated Akt/eNOS signaling pathway. Blunted nitric oxide (NO) synthase with 
      N-nitro-l-arginine methyl ester (L-NAME) can effectively abolish the protective 
      role of Cy3G on endothelial insulin transport and insulin-stimulated glucose 
      utilization in HFD-fed mice. CONCLUSIONS: These findings suggest that Cy3G 
      supplementation can directly restore the attenuated nitic oxide-mediated 
      endothelial insulin transport and thereby ameliorate metabolic insulin 
      resistance. Our finding can provide a novel explanation for the anti-diabetic 
      effects of Cy3G.
CI  - (c) 2021 Wiley-VCH GmbH.
FAU - Geng, Xiuwen
AU  - Geng X
AD  - Department of Nutrition, School of Public Health, Sun Yat-sen University 
      (Northern Campus), Guangzhou, Guangdong Province, 510080, P.R. China.
AD  - Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, 
      Guangdong Province, 510080, P.R. China.
FAU - Ji, Jiajun
AU  - Ji J
AD  - Department of Nutrition, School of Public Health, Sun Yat-sen University 
      (Northern Campus), Guangzhou, Guangdong Province, 510080, P.R. China.
AD  - Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, 
      Guangdong Province, 510080, P.R. China.
FAU - Liu, Yuanhua
AU  - Liu Y
AD  - Department of Nutrition, School of Public Health, Sun Yat-sen University 
      (Northern Campus), Guangzhou, Guangdong Province, 510080, P.R. China.
AD  - Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, 
      Guangdong Province, 510080, P.R. China.
FAU - Li, Xueyan
AU  - Li X
AD  - Department of Nutrition, School of Public Health, Sun Yat-sen University 
      (Northern Campus), Guangzhou, Guangdong Province, 510080, P.R. China.
AD  - Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, 
      Guangdong Province, 510080, P.R. China.
FAU - Chen, Yunan
AU  - Chen Y
AD  - Department of Nutrition, School of Public Health, Sun Yat-sen University 
      (Northern Campus), Guangzhou, Guangdong Province, 510080, P.R. China.
AD  - Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, 
      Guangdong Province, 510080, P.R. China.
FAU - Su, Lei
AU  - Su L
AD  - Department of Nutrition, School of Public Health, Sun Yat-sen University 
      (Northern Campus), Guangzhou, Guangdong Province, 510080, P.R. China.
AD  - Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, 
      Guangdong Province, 510080, P.R. China.
FAU - Zhao, Lina
AU  - Zhao L
AUID- ORCID: 0000-0002-4258-6404
AD  - Department of Nutrition, School of Public Health, Sun Yat-sen University 
      (Northern Campus), Guangzhou, Guangdong Province, 510080, P.R. China.
AD  - Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, 
      Guangdong Province, 510080, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211211
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (Anthocyanins)
RN  - 0 (Glucosides)
RN  - 0 (Insulin)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 7732ZHU564 (cyanidin)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
SB  - IM
MH  - Animals
MH  - Anthocyanins/metabolism/pharmacology
MH  - Dietary Supplements
MH  - Endothelial Cells
MH  - Glucosides/metabolism/pharmacology
MH  - Insulin/metabolism
MH  - *Insulin Resistance
MH  - Mice
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type III/metabolism
OTO - NOTNLM
OT  - cyanidin-3-O-glucoside
OT  - endothelial cells
OT  - insulin resistance
OT  - insulin transport
OT  - nitric oxide
EDAT- 2021/11/30 06:00
MHDA- 2022/04/01 06:00
CRDT- 2021/11/29 06:55
PHST- 2021/10/18 00:00 [revised]
PHST- 2021/08/06 00:00 [received]
PHST- 2021/11/30 06:00 [pubmed]
PHST- 2022/04/01 06:00 [medline]
PHST- 2021/11/29 06:55 [entrez]
AID - 10.1002/mnfr.202100742 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2022 Jan;66(2):e2100742. doi: 10.1002/mnfr.202100742. Epub 
      2021 Dec 11.