PMID- 34841760
OWN - NLM
STAT- MEDLINE
DCOM- 20211130
LR  - 20230829
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 52
IP  - 6
DP  - 2021 Nov
TI  - [Immune Modulatory Effect of Outer Membrane Vesicles Derived from Salmonella on 
      Mouse Bone Marrow-Derived Dendritic Cells].
PG  - 948-953
LID - 10.12182/20210860201 [doi]
AB  - OBJECTIVE: To study the effect of outer membrane vesicles (OMVs) derived from 
      Salmonella typhimurium (ST) on the ultrastructural features and immune function 
      of dendritic cells (DC). METHODS: Mice bone marrow cells were collected 
      aseptically, and myeloid DC were generated by the combined induction and 
      amplification with recombinant mouse granulocyte-macrophage colony-stimulating 
      factor (GM-CSF) and recombinant mouse interleukin-4 (rm IL-4). Cell morphology 
      was observed under inverted phase contrast microscope and the phenotype was 
      identified with flow cytometry. ST-OMVs were isolated through 
      ultracentrifugation. The survival rate of DC was assessed with CCK-8 assay, and 
      the stimulus concentration of OMVs was henceforth determined. The ultrastructural 
      characteristics of DC loaded with OMVs were observed with transmission electron 
      microscopy. The cytokine secretion, surface molecule expression and phagocytic 
      capacity of DC were examined with flow cytometry. RESULTS: The DC induced and 
      amplified in vitro displayed typical DC phenotype in morphological analysis and 
      the purity of DC exceeded 85%. Transmission electron microscopy showed that there 
      were large numbers of protrusions on the cell surface. After stimulation with 
      ST-OMVs, it was observed that the dendritic structures on the surface of DC were 
      reduced and a large number of phagolysosomes were found in the cytoplasm. In 
      addition, increased numbers of mitochondria, swelling and typical apoptosis were 
      observed. After treatment with ST-OMVs at 5 mug/mL and 10 mug/mL, the secretion of 
      tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) of DC increased 
      significantly ( P<0.05). Furthermore, the immature DC could differentiate into 
      mature DCs after stimulation with ST-OMVs, which were characterized by a decrease 
      in phagocytic capacity ( P<0.05) and an upregulation of phenotypic markers ( 
      P<0.05). CONCLUSION: ST-OMVs can stimulate DC to produce TNF-alpha and IL-1beta and 
      promote DC maturation and antigen presentation.
CI  - Copyright(c) by Editorial Board of Journal of Sichuan University (Medical 
      Sciences).
FAU - Wei, Li
AU  - Wei L
AD  - Department of Microbiology and Parasitology, Bengbu Medical College, Bengbu 
      233030, China.
AD  - Anhui Provincial Key Laboratory of Infection and Immunity at Bengbu Medical 
      College, Bengbu 233030, China.
FAU - Chen, Guang-Zhang
AU  - Chen GZ
AD  - Anhui Provincial Key Laboratory of Infection and Immunity at Bengbu Medical 
      College, Bengbu 233030, China.
FAU - Sun, Chao
AU  - Sun C
AD  - Anhui Provincial Key Laboratory of Infection and Immunity at Bengbu Medical 
      College, Bengbu 233030, China.
FAU - Zhang, Tao
AU  - Zhang T
AD  - Department of Microbiology and Parasitology, Bengbu Medical College, Bengbu 
      233030, China.
AD  - Anhui Provincial Key Laboratory of Infection and Immunity at Bengbu Medical 
      College, Bengbu 233030, China.
FAU - Guan, Jun-Chang
AU  - Guan JC
AD  - Department of Microbiology and Parasitology, Bengbu Medical College, Bengbu 
      233030, China.
AD  - Anhui Provincial Key Laboratory of Infection and Immunity at Bengbu Medical 
      College, Bengbu 233030, China.
FAU - Jin, Qi-Li
AU  - Jin QL
AD  - Anhui Provincial Key Laboratory of Infection and Immunity at Bengbu Medical 
      College, Bengbu 233030, China.
AD  - Department of Clinical Laboratory, the Second Affiliated Hospital of Bengbu 
      Medical College, Bengbu 233040, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - *Antigen Presentation
MH  - *Bone Marrow
MH  - Bone Marrow Cells
MH  - Cell Differentiation
MH  - Dendritic Cells
MH  - Granulocyte-Macrophage Colony-Stimulating Factor
MH  - Mice
MH  - Salmonella
PMC - PMC10408817
OTO - NOTNLM
OT  - Dendritic cells
OT  - Outer membrane vesicles
OT  - Salmonella typhimurium
COIS- 利益冲突 　所有作者均声明不存在利益冲突
EDAT- 2021/11/30 06:00
MHDA- 2021/12/01 06:00
PMCR- 2021/11/20
CRDT- 2021/11/29 06:59
PHST- 2021/11/29 06:59 [entrez]
PHST- 2021/11/30 06:00 [pubmed]
PHST- 2021/12/01 06:00 [medline]
PHST- 2021/11/20 00:00 [pmc-release]
AID - scdxxbyxb-52-6-948 [pii]
AID - 10.12182/20210860201 [doi]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2021 Nov;52(6):948-953. doi: 
      10.12182/20210860201.