PMID- 34843633
OWN - NLM
STAT- MEDLINE
DCOM- 20220113
LR  - 20220113
IS  - 1365-2761 (Electronic)
IS  - 0140-7775 (Linking)
VI  - 45
IP  - 2
DP  - 2022 Feb
TI  - Susceptibility of a cell line derived from the kidney of Chinese rice-field eel, 
      Monopterus albus to the infection of rhabdovirus, CrERV.
PG  - 361-371
LID - 10.1111/jfd.13563 [doi]
AB  - Chinese rice-field eels rhabdovirus (CrERV), belonging to the genus Perhabdovirus 
      in the family Rhabdoviridae, is the causative agent of the haemorrhagic disease 
      of Chinese rice-field eels, Monopterus albus. The present study aims to establish 
      a cell line derived from the kidney of Chinese rice-field eel (CrEK) for the 
      further study of the pathogenic virus. CrEK cells were epithelioid-like and grew 
      well in M199 medium supplemented with 10% foetal bovine serum at 28 degrees C, and the 
      cell line has been subcultured for more than 80 times. Karyotyping analysis of 
      CrEK cells at 25th passage indicated a modal chromosome number of 24. Significant 
      cytopathic effect (CPE) was observed in CrEK cells after infection with CrERV, 
      and the virus titre reached 10(7.8 +/- 0.45) TCID(50) /mL. The transmission 
      electron microscopy revealed that there were a large number of virus particles in 
      the cytoplasm of cells. The virus infection in cells was also assayed by using 
      indirect immunofluorescence assay (IFA), fluorescence in situ hybridization 
      (FISH), reverse transcription PCR (RT-PCR) and quantitative real-time reverse 
      transcription-PCR (qRT-PCR). In experimental infection, CrERV cultured by cells 
      could cause over 90% mortality in fish. CrEK represents the first kidney cell 
      line originated from Chinese rice-field eels and be a potential material for 
      investigating the mechanism of virus infection in this fish and the control 
      methods for the disease.
CI  - (c) 2021 John Wiley & Sons Ltd.
FAU - Liu, Wenzhi
AU  - Liu W
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan, China.
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan, China.
FAU - Fan, Yuding
AU  - Fan Y
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan, China.
FAU - Zhou, Yong
AU  - Zhou Y
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan, China.
FAU - Jiang, Nan
AU  - Jiang N
AUID- ORCID: 0000-0001-8460-3949
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan, China.
FAU - Li, Yiqun
AU  - Li Y
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan, China.
FAU - Meng, Yan
AU  - Meng Y
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan, China.
FAU - Xue, Mingyang
AU  - Xue M
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan, China.
FAU - Li, Zhong
AU  - Li Z
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan, China.
FAU - Zeng, Lingbing
AU  - Zeng L
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan, China.
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan, China.
LA  - eng
SI  - GENBANK/LC151290
SI  - GENBANK/MH319839
GR  - National Key Research and Development Program of China/
PT  - Journal Article
DEP - 20211129
PL  - England
TA  - J Fish Dis
JT  - Journal of fish diseases
JID - 9881188
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - China
MH  - Eels
MH  - *Fish Diseases
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney
MH  - *Rhabdoviridae/genetics
OTO - NOTNLM
OT  - Chinese rice-field eels
OT  - CrEK cell line
OT  - CrERV
OT  - susceptibility
OT  - viral replication
EDAT- 2021/11/30 06:00
MHDA- 2022/01/14 06:00
CRDT- 2021/11/29 17:27
PHST- 2021/11/13 00:00 [revised]
PHST- 2021/08/23 00:00 [received]
PHST- 2021/11/16 00:00 [accepted]
PHST- 2021/11/30 06:00 [pubmed]
PHST- 2022/01/14 06:00 [medline]
PHST- 2021/11/29 17:27 [entrez]
AID - 10.1111/jfd.13563 [doi]
PST - ppublish
SO  - J Fish Dis. 2022 Feb;45(2):361-371. doi: 10.1111/jfd.13563. Epub 2021 Nov 29.