PMID- 34846850
OWN - NLM
STAT- MEDLINE
DCOM- 20220106
LR  - 20220106
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 55
IP  - 24
DP  - 2021 Dec 21
TI  - Characterization of Microplastic-Associated Biofilm Development along a 
      Freshwater-Estuarine Gradient.
PG  - 16402-16412
LID - 10.1021/acs.est.1c04108 [doi]
AB  - Microplastic contamination is an increasing concern worldwide. Biofilms rapidly 
      develop on surfaces in aquatic habitats, but the processes of biofilm formation 
      and variation in bacterial community succession on different microplastics 
      introduced into freshwater and estuarine environments are not well understood. In 
      this study, the biofilm bacterial communities that developed on three different 
      types of microplastics that are prevalent in the environment, high-density 
      polyethylene (HDPE), polyethylene terephthalate (PET), and polystyrene (PS), was 
      investigated. Virgin microplastics were incubated in microcosms over a period of 
      31 days with water collected along a freshwater-estuarine gradient of the Raritan 
      River in New Jersey. Through long-read MinION sequencing of bacterial ribosomal 
      operons, we were able to examine biofilm bacterial communities at a species- and 
      strain-level resolution. Results indicated that both salinity level and 
      microplastic type impacted biofilm formation and promoted colonization by 
      distinct microbial communities. Limnobacter thiooxidans was found to be one of 
      the most abundant microplastics colonizing-bacteria, and it is hypothesized that 
      different types of microplastics could select for different strains. Our findings 
      indicate that multiple groups of highly similar L. thiooxidans rRNA operons could 
      be discerned within the community profiles. Phylogenetic reconstruction further 
      established that various Linmobacter species uniquely colonized the different 
      microplastics from the different sampling sites. Our findings indicate that 
      microplastics support abundant and diverse bacterial communities and that the 
      various types of microplastics can influence how different bacterial biofilms 
      develop, which may have ecological impacts on aquatic ecosystems.
FAU - Qiang, Liyuan
AU  - Qiang L
AD  - Department of Biochemistry and Microbiology, School of Environmental and 
      Biological Sciences, Rutgers, The State University of New Jersey, 76 Lipman 
      Drive, New Brunswick, New Jersey 08901-8525, United States.
AD  - College of Mechanical and Electrical Engineering, Shihezi University, Xinjiang 
      832003, China.
AD  - State Key Laboratory of Estuarine and Coastal Research, East China Normal 
      University, Shanghai 200062, China.
FAU - Cheng, Jinping
AU  - Cheng J
AUID- ORCID: 0000-0003-3585-6645
AD  - Hong Kong Branch of the Southern Marine Science and Engineering Guangdong 
      Laboratory (Guangzhou), State Key Laboratory of Marine Pollution & Department of 
      Ocean Science, The Hong Kong University of Science and Technology, Clear Water 
      Bay, Hong Kong, China.
FAU - Mirzoyan, Seda
AU  - Mirzoyan S
AD  - Department of Marine and Coastal Sciences, School of Environmental and Biological 
      Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New 
      Brunswick, New Jersey 08901-8521, United States.
AD  - Department of Zoology, Faculty of Science, Charles University, Prague 128 00, 
      Czech Republic.
FAU - Kerkhof, Lee J
AU  - Kerkhof LJ
AUID- ORCID: 0000-0001-9107-8695
AD  - Department of Marine and Coastal Sciences, School of Environmental and Biological 
      Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New 
      Brunswick, New Jersey 08901-8521, United States.
FAU - Haggblom, Max M
AU  - Haggblom MM
AUID- ORCID: 0000-0001-6307-7863
AD  - Department of Biochemistry and Microbiology, School of Environmental and 
      Biological Sciences, Rutgers, The State University of New Jersey, 76 Lipman 
      Drive, New Brunswick, New Jersey 08901-8525, United States.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20211130
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Microplastics)
RN  - 0 (Plastics)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Biofilms
MH  - Environmental Monitoring
MH  - *Microbiota
MH  - Microplastics
MH  - Phylogeny
MH  - Plastics
MH  - Rivers
MH  - *Water Pollutants, Chemical/analysis
OTO - NOTNLM
OT  - Oxford Nanopore MinION
OT  - aquatic
OT  - bacterial community analysis
OT  - biofilm
OT  - microplastics
EDAT- 2021/12/01 06:00
MHDA- 2022/01/07 06:00
CRDT- 2021/11/30 17:11
PHST- 2021/12/01 06:00 [pubmed]
PHST- 2022/01/07 06:00 [medline]
PHST- 2021/11/30 17:11 [entrez]
AID - 10.1021/acs.est.1c04108 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2021 Dec 21;55(24):16402-16412. doi: 
      10.1021/acs.est.1c04108. Epub 2021 Nov 30.