PMID- 34850551
OWN - NLM
STAT- MEDLINE
DCOM- 20220331
LR  - 20220531
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 26
IP  - 2
DP  - 2022 Jan
TI  - ALKBH5-mediated m6A modification of lncRNA KCNQ1OT1 triggers the development of 
      LSCC via upregulation of HOXA9.
PG  - 385-398
LID - 10.1111/jcmm.17091 [doi]
AB  - It has been shown that N6-methyladenosine (m6A) modification is involved in the 
      development of complex human diseases, especially in the development of cancer. 
      Our research investigated the role and mechanism of the m6A modification of 
      lncRNA KCNQ1 overlapping transcript 1 (KCNQ1OT1) in Laryngeal squamous cell 
      carcinoma (LSCC) progression. Microarray analysis was used to quantitatively 
      detect the m6A apparent transcriptional modification level of lncRNA in LSCC 
      tissue. Methylated RNA immunoprecipitation-qPCR (MeRIP-qPCR), in situ 
      hybridization (ISH) and quantitative real-time PCR (qRT-PCR) were used to examine 
      the m6A modification and expression of KCNQ1OT1. In addition, in vivo and in 
      vitro experiments have tested the effects of KCNQ1OT1 knockdown on the 
      proliferation, invasion and metastasis of LSCC. Mechanically, we found the 
      N6-methyladenosine (m6A) demethylase ALKBH5 mediates KCNQ1OT1 expression via an 
      m6A-YTHDF2-dependent manner and KCNQ1OT1 could directly bind to HOXA9 to further 
      regulate the proliferation, invasion and metastasis of LSCC cells. In general, 
      our research indicates that ALKBH5-mediated m6A modification of KCNQ1OT1 triggers 
      the development of LSCC via upregulation of HOXA9.
CI  - (c) 2021 The Authors. Journal of Cellular and Molecular Medicine published by 
      Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
FAU - Li, Yushan
AU  - Li Y
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, China.
FAU - Yan, Bingrui
AU  - Yan B
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, China.
FAU - Wang, Xin
AU  - Wang X
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, China.
FAU - Li, Qiuying
AU  - Li Q
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, China.
FAU - Kan, Xuan
AU  - Kan X
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, China.
FAU - Wang, Jingting
AU  - Wang J
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, China.
FAU - Sun, Yanan
AU  - Sun Y
AUID- ORCID: 0000-0003-4587-3297
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, China.
FAU - Wang, Peng
AU  - Wang P
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, China.
FAU - Tian, Linli
AU  - Tian L
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, China.
FAU - Liu, Ming
AU  - Liu M
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211201
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (RNA, Long Noncoding)
RN  - EC 1.14.11.- (ALKBH5 protein, human)
RN  - EC 1.14.11.- (AlkB Homolog 5, RNA Demethylase)
SB  - IM
MH  - AlkB Homolog 5, RNA Demethylase/genetics/metabolism
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - *RNA, Long Noncoding/genetics
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Up-Regulation/genetics
PMC - PMC8743647
OTO - NOTNLM
OT  - ALKBH5
OT  - HOXA9
OT  - laryngeal squamous cell cancer
OT  - lncRNA KCNQ1OT1
OT  - m6A methylation
COIS- The authors declare that they have no competing interests.
EDAT- 2021/12/02 06:00
MHDA- 2022/04/01 06:00
PMCR- 2022/01/01
CRDT- 2021/12/01 07:49
PHST- 2021/10/29 00:00 [revised]
PHST- 2021/07/13 00:00 [received]
PHST- 2021/11/11 00:00 [accepted]
PHST- 2021/12/02 06:00 [pubmed]
PHST- 2022/04/01 06:00 [medline]
PHST- 2021/12/01 07:49 [entrez]
PHST- 2022/01/01 00:00 [pmc-release]
AID - JCMM17091 [pii]
AID - 10.1111/jcmm.17091 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2022 Jan;26(2):385-398. doi: 10.1111/jcmm.17091. Epub 2021 Dec 1.