PMID- 34857792
OWN - NLM
STAT- MEDLINE
DCOM- 20220120
LR  - 20221027
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Dec 2
TI  - A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive 
      droplet-based photonic PCR.
PG  - 23338
LID - 10.1038/s41598-021-02535-1 [doi]
LID - 23338
AB  - Polymerase chain reaction (PCR) is a powerful tool for nucleic acid amplification 
      and quantification. However, long thermocycling time is a major limitation of the 
      commercial PCR devices in the point-of-care (POC). Herein, we have developed a 
      rapid droplet-based photonic PCR (dpPCR) system, including a gold (Au) 
      nanofilm-based microfluidic chip and a plasmonic photothermal cycler. The chip is 
      fabricated by adding mineral oil to uncured polydimethylsiloxane (PDMS) to 
      suppress droplet evaporation in PDMS microfluidic chips during PCR thermocycling. 
      A PDMS to gold bonding technique using a double-sided adhesive tape is applied to 
      enhance the bonding strength between the oil-added PDMS and the gold nanofilm. 
      Moreover, the gold nanofilm excited by two light-emitting diodes (LEDs) from the 
      top and bottom sides of the chip provides fast heating of the PCR sample to 
      230  degrees C within 100 s. Such a design enables 30 thermal cycles from 60 to 95  degrees C 
      within 13 min with the average heating and cooling rates of 7.37 +/- 0.27  degrees C/s and 
      1.91 +/- 0.03  degrees C/s, respectively. The experimental results demonstrate successful 
      PCR amplification of the alcohol oxidase (AOX) gene using the rapid plasmonic 
      photothermal cycler and exhibit the great performance of the microfluidic chip 
      for droplet-based PCR.
CI  - (c) 2021. The Author(s).
FAU - Jalili, Abbas
AU  - Jalili A
AD  - Department of Mechanical Engineering, Sharif University of Technology, Azadi 
      Ave., Tehran, Iran.
FAU - Bagheri, Maryam
AU  - Bagheri M
AD  - Department of Mechanical Engineering, Sharif University of Technology, Azadi 
      Ave., Tehran, Iran.
FAU - Shamloo, Amir
AU  - Shamloo A
AD  - Department of Mechanical Engineering, Sharif University of Technology, Azadi 
      Ave., Tehran, Iran. shamloo@sharif.edu.
FAU - Kazemipour Ashkezari, Amir Hossein
AU  - Kazemipour Ashkezari AH
AD  - Department of Mechanical Engineering, Sharif University of Technology, Azadi 
      Ave., Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20211202
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Dimethylpolysiloxanes)
RN  - 63148-62-9 (baysilon)
RN  - 7440-57-5 (Gold)
RN  - EC 1.2.3.1 (AOX1 protein, human)
RN  - EC 1.2.3.1 (Aldehyde Oxidase)
SB  - IM
MH  - Aldehyde Oxidase/*analysis/genetics
MH  - Dimethylpolysiloxanes/chemistry
MH  - Gold/*chemistry
MH  - Humans
MH  - Lab-On-A-Chip Devices/*statistics & numerical data
MH  - Microfluidic Analytical Techniques/*instrumentation
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - *Photons
MH  - Polymerase Chain Reaction/*methods
PMC - PMC8639772
COIS- A provisional U.S. patent on droplet-based photonic PCR device used for this 
      study has been filed by Amir Shamloo (application number: 63254176). A.S., A.J., 
      and M.B. have been listed as inventors of this pending patent. A.K. declares no 
      competing interests.
EDAT- 2021/12/04 06:00
MHDA- 2022/01/21 06:00
PMCR- 2021/12/02
CRDT- 2021/12/03 06:26
PHST- 2021/07/29 00:00 [received]
PHST- 2021/11/12 00:00 [accepted]
PHST- 2021/12/03 06:26 [entrez]
PHST- 2021/12/04 06:00 [pubmed]
PHST- 2022/01/21 06:00 [medline]
PHST- 2021/12/02 00:00 [pmc-release]
AID - 10.1038/s41598-021-02535-1 [pii]
AID - 2535 [pii]
AID - 10.1038/s41598-021-02535-1 [doi]
PST - epublish
SO  - Sci Rep. 2021 Dec 2;11(1):23338. doi: 10.1038/s41598-021-02535-1.