PMID- 3485855
OWN - NLM
STAT- MEDLINE
DCOM- 19860507
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 150
IP  - 2
DP  - 1986 Apr 30
TI  - Polypeptides of Mason-Pfizer monkey virus. III. Translational order of proteins 
      on the gag and env gene specified precursor polypeptides.
PG  - 503-8
AB  - Mason-Pfizer monkey virus (M-PMV), the prototype D-type retrovirus, unlike most 
      retroviruses, preassembles core structures in the cytoplasm of infected cells 
      during morphogenesis. We have shown previously (J. Bradac and E. Hunter, 1984, 
      Virology 138, 260-275) that M-PMV virions contain 5 gag-encoded polypeptides. In 
      this report, the translational order of the proteins encoded on the gag and env 
      genes of M-PMV has been determined by pactamycin mapping. The proposed order of 
      the viral proteins on their respective precursors is env: NH2-gp85-gp20-COOH; 
      gag: NH2-p10-pp24/pp16-p12-p27-p14-COOH. Myristic acid-labeled virions contained 
      a single radioactive protein, p10, supporting the mapping of this molecule to the 
      amino terminus. From these studies it is clear that M-PMV contains an unusual 
      additional gag polypeptide, p12, for which no function has been assigned to date. 
      A remarkable similarity exists in the size and organization of M-PMV and mouse 
      mammary tumor virus (MMTV) gag polypeptides; suggesting a common ancestor to 
      these two viruses.
FAU - Bradac, J A
AU  - Bradac JA
FAU - Hunter, E
AU  - Hunter E
LA  - eng
GR  - CA 00685/CA/NCI NIH HHS/United States
GR  - CA 09202/CA/NCI NIH HHS/United States
GR  - CA 27834/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Protein Precursors)
RN  - 0 (Viral Proteins)
RN  - 23668-11-3 (Pactamycin)
SB  - IM
MH  - Animals
MH  - *Genes, Viral
MH  - Macaca mulatta
MH  - Pactamycin/pharmacology
MH  - *Peptide Biosynthesis
MH  - *Protein Biosynthesis
MH  - Protein Precursors/biosynthesis/*genetics
MH  - Retroviridae/*genetics/metabolism
MH  - Viral Proteins/*biosynthesis/genetics
EDAT- 1986/04/30 00:00
MHDA- 1986/04/30 00:01
CRDT- 1986/04/30 00:00
PHST- 1986/04/30 00:00 [pubmed]
PHST- 1986/04/30 00:01 [medline]
PHST- 1986/04/30 00:00 [entrez]
AID - 10.1016/0042-6822(86)90314-4 [doi]
PST - ppublish
SO  - Virology. 1986 Apr 30;150(2):503-8. doi: 10.1016/0042-6822(86)90314-4.