PMID- 34858874
OWN - NLM
STAT- MEDLINE
DCOM- 20220127
LR  - 20220127
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 11
DP  - 2021
TI  - Molecular Epidemiological Survey of Staphylococcus lugdunensis Isolates With 
      Variable Number of Repeats in the von Willebrand Factor-Binding Protein Gene.
PG  - 748640
LID - 10.3389/fcimb.2021.748640 [doi]
LID - 748640
AB  - The von Willebrand factor binding protein in Staphylococcus lugdunensis (vWbl) 
      comprises four major regions: the signal peptide (S), the non-repetitive (A) 
      region, the repeat (R) region, and the wall-associated (W) region. Previous 
      studies have demonstrated that the R region contains 10 copies of repeating 
      sequences; however, we reveal that the copy number of repeats in the vWbl gene 
      varies among different S. lugdunensis isolates. In this study, an epidemiological 
      surveillance was conducted to determine whether the copy number of repeats in 
      vWbl in different isolates of S. lugdunensis correlates with their infectivity. 
      The number of repeats was estimated in a total of 212 isolates, consisting of 162 
      isolates of oxacillin-sensitive S. lugdunensis (OSSL) and 50 isolates of 
      oxacillin-resistant S. lugdunensis (ORSL). Our data showed that 72.5% (116/162) 
      of OSSL isolates contained 9 (25, 15.4%), 12 (43, 26.5%), or 13 (48, 29.6%) 
      repeats, and 90% (45/50) of ORSL isolates had 9 (32, 64%) or 13 (13, 26%) 
      repeats. In addition, 89.6% (26 of 29) of the sequence type (ST)27 strain had 12 
      repeats, and 86.8% (13 of 15) of the ST4 strain had 14 repeats. Twenty-seven of 
      the 28 isolates with nine repeats were of the staphylococcal cassette chromosome 
      mec (SCCmec) V or V(t) type and belonged to ST3, and all isolates with 13 repeats 
      were of SCCmec II type and belonged to ST6. All isolates with nine repeats had a 
      stop codon at the 18(th) codon of the third repeat, suggesting that these 
      isolates coded for nonfunctional vWbl. Further, western blot analysis confirmed 
      that all strains translated vWbl, and only vWbl proteins coded by genes with nine 
      repeats were exported outside the cell. These results suggest that number of vWbl 
      repeats in S. lugdunensis have clonal specificities and may correlate with 
      potential pathogenicity.
CI  - Copyright (c) 2021 Lin, Cheng, Chang and Lu.
FAU - Lin, Lee-Chung
AU  - Lin LC
AD  - Department of Laboratory Medicine, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
FAU - Cheng, Chun-Wen
AU  - Cheng CW
AD  - Division of Infectious Diseases, Department of Internal Medicine, Chang Gung 
      Memorial Hospital at Linkou, Chang Gung University College of Medicine, Taoyuan, 
      Taiwan.
FAU - Chang, Shih-Cheng
AU  - Chang SC
AD  - Department of Laboratory Medicine, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
AD  - Department of Medical Biotechnology and Laboratory Science, College of Medicine, 
      Chang Gung University, Taoyuan, Taiwan.
FAU - Lu, Jang-Jih
AU  - Lu JJ
AD  - Department of Laboratory Medicine, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
AD  - Department of Medical Biotechnology and Laboratory Science, College of Medicine, 
      Chang Gung University, Taoyuan, Taiwan.
AD  - Department of Medicine, College of Medicine, Chang Gung University, Taoyuan, 
      Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211111
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Carrier Proteins)
RN  - 0 (von Willebrand Factor)
SB  - IM
MH  - Anti-Bacterial Agents
MH  - Carrier Proteins/genetics
MH  - Humans
MH  - *Methicillin-Resistant Staphylococcus aureus
MH  - Microbial Sensitivity Tests
MH  - *Staphylococcal Infections/epidemiology
MH  - *Staphylococcus lugdunensis/genetics
MH  - von Willebrand Factor
PMC - PMC8632046
OTO - NOTNLM
OT  - MLST
OT  - S. lugdunensis
OT  - SCCmec
OT  - pathogenesis
OT  - von Willebrand factor binding protein
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/12/04 06:00
MHDA- 2022/01/28 06:00
PMCR- 2021/01/01
CRDT- 2021/12/03 07:07
PHST- 2021/07/28 00:00 [received]
PHST- 2021/10/27 00:00 [accepted]
PHST- 2021/12/03 07:07 [entrez]
PHST- 2021/12/04 06:00 [pubmed]
PHST- 2022/01/28 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2021.748640 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2021 Nov 11;11:748640. doi: 
      10.3389/fcimb.2021.748640. eCollection 2021.