PMID- 34859055
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220430
IS  - 2296-889X (Print)
IS  - 2296-889X (Electronic)
IS  - 2296-889X (Linking)
VI  - 8
DP  - 2021
TI  - Multiplexed Single-Cell in situ RNA Profiling.
PG  - 775410
LID - 10.3389/fmolb.2021.775410 [doi]
LID - 775410
AB  - The ability to quantify a large number of varied transcripts in single cells in 
      their native spatial context is crucial to accelerate our understanding of health 
      and disease. Bulk cell RNA analysis masks the heterogeneity in the cell 
      population, while the conventional RNA imaging approaches suffer from low 
      multiplexing capacity. Recent advances in multiplexed fluorescence in situ 
      hybridization (FISH) methods enable comprehensive RNA profiling in individual 
      cells in situ. These technologies will have wide applications in many biological 
      and biomedical fields, including cell type classification, signaling network 
      analysis, tissue architecture, disease diagnosis and patient stratification, etc. 
      In this minireview, we will present the recent technological advances of 
      multiplexed single-cell in situ RNA profiling assays, discuss their advantages 
      and limitations, describe their biological applications, highlight the current 
      challenges, and propose potential solutions.
CI  - Copyright (c) 2021 Wang and Guo.
FAU - Wang, Yu-Sheng
AU  - Wang YS
AD  - Biodesign Institute and School of Molecular Sciences, Arizona State University, 
      Tempe, AZ, United States.
FAU - Guo, Jia
AU  - Guo J
AD  - Biodesign Institute and School of Molecular Sciences, Arizona State University, 
      Tempe, AZ, United States.
LA  - eng
GR  - R01 GM127633/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Review
DEP - 20211111
PL  - Switzerland
TA  - Front Mol Biosci
JT  - Frontiers in molecular biosciences
JID - 101653173
PMC - PMC8632036
OTO - NOTNLM
OT  - fish
OT  - fluorescence in situ hybridization
OT  - genomics
OT  - transcriptomics
OT  - transcripts
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/12/04 06:00
MHDA- 2021/12/04 06:01
PMCR- 2021/01/01
CRDT- 2021/12/03 07:08
PHST- 2021/09/14 00:00 [received]
PHST- 2021/10/26 00:00 [accepted]
PHST- 2021/12/03 07:08 [entrez]
PHST- 2021/12/04 06:00 [pubmed]
PHST- 2021/12/04 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 775410 [pii]
AID - 10.3389/fmolb.2021.775410 [doi]
PST - epublish
SO  - Front Mol Biosci. 2021 Nov 11;8:775410. doi: 10.3389/fmolb.2021.775410. 
      eCollection 2021.