PMID- 34859450
OWN - NLM
STAT- MEDLINE
DCOM- 20220404
LR  - 20220405
IS  - 1600-065X (Electronic)
IS  - 0105-2896 (Linking)
VI  - 306
IP  - 1
DP  - 2022 Mar
TI  - Imaging dendritic cell functions.
PG  - 137-163
LID - 10.1111/imr.13050 [doi]
AB  - Dendritic cells (DCs) are crucial for the appropriate initiation of adaptive 
      immune responses. During inflammation, DCs capture antigens, mature, and migrate 
      to lymphoid tissues to present foreign material to naive T cells. These cells get 
      activated and differentiate either into pathogen-specific cytotoxic CD8(+) T 
      cells that destroy infected cells or into CD4(+) T helper cells that, among other 
      effector functions, orchestrate antibody production by B cells. DC-mediated 
      antigen presentation is equally important in non-inflammatory conditions. Here, 
      DCs mediate induction of tolerance by presenting self-antigens or harmless 
      environmental antigens and induce differentiation of regulatory T cells or 
      inactivation of self-reactive immune cells. Detailed insights into the biology of 
      DCs are, therefore, crucial for the development of novel vaccines as well as the 
      prevention of autoimmune diseases. As in many other life science areas, our 
      understanding of DC biology would be extremely restricted without bioimaging, a 
      compilation of methods that visualize biological processes. Spatiotemporal 
      tracking of DCs relies on various imaging tools, which not only enable insights 
      into their positioning and migration within tissues or entire organs but also 
      allow visualization of subcellular and molecular processes. This review aims to 
      provide an overview of the imaging toolbox and to provide examples of diverse 
      imaging techniques used to obtain fundamental insights into DC biology.
CI  - (c) 2021 The Authors. Immunological Reviews published by John Wiley & Sons Ltd.
FAU - Bosnjak, Berislav
AU  - Bosnjak B
AUID- ORCID: 0000-0003-3374-7488
AD  - Institute of Immunology, Hannover Medical School, Hannover, Germany.
FAU - Do, Kim Thi Hoang
AU  - Do KTH
AD  - Institute of Immunology, Hannover Medical School, Hannover, Germany.
FAU - Forster, Reinhold
AU  - Forster R
AUID- ORCID: 0000-0001-6190-7923
AD  - Institute of Immunology, Hannover Medical School, Hannover, Germany.
AD  - Cluster of Excellence RESIST (EXC 2155) Hannover Medical School, Hannover, 
      Germany.
AD  - German Centre for Infection Research (DZIF), Hannover, Germany.
FAU - Hammerschmidt, Swantje I
AU  - Hammerschmidt SI
AD  - Institute of Immunology, Hannover Medical School, Hannover, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20211203
PL  - England
TA  - Immunol Rev
JT  - Immunological reviews
JID - 7702118
SB  - IM
MH  - Antigen Presentation
MH  - *CD8-Positive T-Lymphocytes
MH  - *Dendritic Cells
MH  - Humans
MH  - Immune Tolerance
MH  - T-Lymphocytes, Cytotoxic
OTO - NOTNLM
OT  - antigen presentation
OT  - dendritic cells (DCs)
OT  - imaging
OT  - immune response
OT  - maturation
OT  - migration
EDAT- 2021/12/04 06:00
MHDA- 2022/04/05 06:00
CRDT- 2021/12/03 07:20
PHST- 2021/11/16 00:00 [revised]
PHST- 2021/10/25 00:00 [received]
PHST- 2021/11/18 00:00 [accepted]
PHST- 2021/12/04 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2021/12/03 07:20 [entrez]
AID - 10.1111/imr.13050 [doi]
PST - ppublish
SO  - Immunol Rev. 2022 Mar;306(1):137-163. doi: 10.1111/imr.13050. Epub 2021 Dec 3.