PMID- 34862469
OWN - NLM
STAT- MEDLINE
DCOM- 20220121
LR  - 20220121
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Dec 3
TI  - Integrated metabolic and microbial analysis reveals host-microbial interactions 
      in IgE-mediated childhood asthma.
PG  - 23407
LID - 10.1038/s41598-021-02925-5 [doi]
LID - 23407
AB  - A metabolomics-based approach to address the molecular mechanism of childhood 
      asthma with immunoglobulin E (IgE) or allergen sensitization related to 
      microbiome in the airways remains lacking. Fifty-three children with lowly 
      sensitized non-atopic asthma (n = 15), highly sensitized atopic asthma (n = 13), 
      and healthy controls (n = 25) were enrolled. Blood metabolomic analysis with 
      (1)H-nuclear magnetic resonance (NMR) spectroscopy and airway microbiome 
      composition analysis by bacterial 16S rRNA sequencing were performed. An 
      integrative analysis of their associations with allergen-specific IgE levels for 
      lowly and highly sensitized asthma was also assessed. Four metabolites including 
      tyrosine, isovalerate, glycine, and histidine were uniquely associated with lowly 
      sensitized asthma, whereas one metabolite, acetic acid, was strongly associated 
      with highly sensitized asthma. Metabolites associated with highly sensitized 
      asthma (valine, isobutyric acid, and acetic acid) and lowly sensitized asthma 
      (isovalerate, tyrosine, and histidine) were strongly correlated each other 
      (P < 0.01). Highly sensitized asthma associated metabolites were mainly enriched 
      in pyruvate and acetyl-CoA metabolisms. Metabolites associated with highly 
      sensitized atopic asthma were mostly correlated with microbiota in the airways. 
      Acetic acid, a short-chain fatty acid (SCFA), was negatively correlated with the 
      genus Atopobium (P < 0.01), but positively correlated with the genus 
      Fusobacterium (P < 0.05). In conclusion, metabolomics reveals microbes-related 
      metabolic pathways associated with IgE responses to house dust mite allergens in 
      childhood asthma. A strong correlation of metabolites related to highly 
      sensitized atopic asthma with airway microbiota provides linkages between the 
      host-microbial interactions and asthma endotypes.
CI  - (c) 2021. The Author(s).
FAU - Chiu, Chih-Yung
AU  - Chiu CY
AD  - Division of Pediatric Pulmonology, College of Medicine, Chang Gung Memorial 
      Hospital at Linkou, Chang Gung University, Taoyuan, Taiwan. pedchestic@gmail.com.
AD  - Clinical Metabolomics Core Laboratory, Chang Gung Memorial Hospital at Linkou, 
      Taoyuan, Taiwan. pedchestic@gmail.com.
FAU - Cheng, Mei-Ling
AU  - Cheng ML
AD  - Clinical Metabolomics Core Laboratory, Chang Gung Memorial Hospital at Linkou, 
      Taoyuan, Taiwan.
AD  - Department of Biomedical Sciences, and Metabolomics Core Laboratory, Healthy 
      Aging Research Center, College of Medicine, Chang Gung University, Taoyuan, 
      Taiwan.
FAU - Chiang, Meng-Han
AU  - Chiang MH
AD  - Department of Medical Imaging and Intervention, Imaging Core Laboratory, 
      Institute for Radiological Research, and Clinical Metabolomics Core Laboratory, 
      College of Medicine, Chang Gung Memorial Hospital at Linkou, Chang Gung 
      University, Taoyuan, Taiwan.
FAU - Wang, Chia-Jung
AU  - Wang CJ
AD  - Division of Pediatric Pulmonology, College of Medicine, Chang Gung Memorial 
      Hospital at Linkou, Chang Gung University, Taoyuan, Taiwan.
FAU - Tsai, Ming-Han
AU  - Tsai MH
AD  - Department of Pediatrics, College of Medicine, Chang Gung Memorial Hospital at 
      Keelung, Chang Gung University, Taoyuan, Taiwan.
FAU - Lin, Gigin
AU  - Lin G
AD  - Department of Medical Imaging and Intervention, Imaging Core Laboratory, 
      Institute for Radiological Research, and Clinical Metabolomics Core Laboratory, 
      College of Medicine, Chang Gung Memorial Hospital at Linkou, Chang Gung 
      University, Taoyuan, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211203
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Asthma/blood/*immunology/microbiology
MH  - Bacteria/*classification/genetics/isolation & purification
MH  - Case-Control Studies
MH  - Child, Preschool
MH  - Cross-Sectional Studies
MH  - DNA, Bacterial/genetics
MH  - DNA, Ribosomal/genetics
MH  - Female
MH  - Host Microbial Interactions
MH  - Humans
MH  - Immunoglobulin E/*blood/immunology
MH  - Male
MH  - Metabolic Networks and Pathways
MH  - Metabolomics/*methods
MH  - Phylogeny
MH  - Proton Magnetic Resonance Spectroscopy
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA/*methods
PMC - PMC8642522
COIS- The authors declare no competing interests.
EDAT- 2021/12/05 06:00
MHDA- 2022/01/22 06:00
PMCR- 2021/12/03
CRDT- 2021/12/04 06:05
PHST- 2021/05/26 00:00 [received]
PHST- 2021/11/24 00:00 [accepted]
PHST- 2021/12/04 06:05 [entrez]
PHST- 2021/12/05 06:00 [pubmed]
PHST- 2022/01/22 06:00 [medline]
PHST- 2021/12/03 00:00 [pmc-release]
AID - 10.1038/s41598-021-02925-5 [pii]
AID - 2925 [pii]
AID - 10.1038/s41598-021-02925-5 [doi]
PST - epublish
SO  - Sci Rep. 2021 Dec 3;11(1):23407. doi: 10.1038/s41598-021-02925-5.