PMID- 34865325
OWN - NLM
STAT- MEDLINE
DCOM- 20211207
LR  - 20211214
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 46
IP  - 11
DP  - 2021 Nov 25
TI  - [Electroacupuncture at "Zusanli"(ST36) ameliorates tau hyperphosphorylation in 
      pancreas and hippocampus of diabetic rats].
PG  - 901-6
LID - 10.13702/j.1000-0607.200921 [doi]
AB  - OBJECTIVE: To observe the effect of electroacupuncture (EA) at "Zusanli"(ST36) on 
      the phosphorylated tau levels in pancreas and hippocampus of type 2 diabetes 
      mellitus (T2DM) rats，so as to explore the underlying mechanism of EA in diabetic 
      demention rats. METHODS: Forty-eight male Sprague-Dawley rats were randomly 
      divided into control, model and EA groups, with 16 rats in each group. The T2DM 
      model was established by 6 weeks of high-fat, high-sugar diet as well as 
      intrape-ritoneal injection of streptozocin (STZ) solution (35 mg/kg). After that, 
      EA (2 Hz, 0.1 mA) was applied to unilateral "Zusanli"(ST36) for 30 min, once a 
      day, 6 times a week for 4 weeks. The survival rate was recorded every week, and 
      the fasting blood glucose (FBG) was detected on the 1st, 6th and 11th week. The 
      level of serum insulin (INS) was measured by using ELISA. The morphological 
      structure of pancreas islet was observed by H.E. staining. The expressions of 
      phosphorylated tau at the sites of Ser 396 (pS396) and Thr 231 (pT231), total tau 
      (Tau5), phosphorylated glycogen synthase kinase-3beta (pGSK-3beta) and total glycogen 
      synthase kinase-3beta (GSK-3beta) in pancreas and hippocampus were detected by Western 
      blot. The expression and distribution of pS396 and pT231 in pancreas and 
      hippocampus were assayed with immunohistochemistry. RESULTS: Compared with the 
      control group, the survival rate presented a significant decline, the contents of 
      FBG and INS were obviously higher(P<0.01), and the structure of the pancreas 
      islet appeared shrunken, obscure and disordered in the model group. Furthermore, 
      the levels of pS396, pT231 in pancreas and hippocampus were obviously higher in 
      the model group(P<0.01)，while the level of pGSK-3beta in pancreas and hippocampus 
      was significantly lower in the model group(P<0.01). In comparison with the model 
      group, the survival rate of EA group was higher. Following 4 weeks' 
      interventions, the enhanced levels of tau phosphorylation and GSK-3beta activity in 
      pancreas and hippocampus were partly reversed in the EA group compared to the 
      model group(P<0.05，P<0.01). CONCLUSION: EA at ST36 can reduce the level of tau 
      phosphorylation via regulating the activity of GSK-3beta in the pancreas and 
      hippocampus of T2DM rats, which may be related with the effect of EA on the brain 
      function in T2DM rats.
FAU - Yuan, Fang
AU  - Yuan F
AD  - College of Basic Medicine, Hubei University of Chinese Medicine, Wuhan 430065, 
      China.
FAU - Hong, Xiao-Ping
AU  - Hong XP
AD  - College of Basic Medicine, Hubei University of Chinese Medicine, Wuhan 430065, 
      China.
FAU - Duan, Yan-Jun
AU  - Duan YJ
AD  - College of Basic Medicine, Hubei University of Chinese Medicine, Wuhan 430065, 
      China.
FAU - Chen, Jiao-Rong
AU  - Chen JR
AD  - College of Basic Medicine, Hubei University of Chinese Medicine, Wuhan 430065, 
      China.
FAU - Han, Yong-Ming
AU  - Han YM
AD  - College of Basic Medicine, Hubei University of Chinese Medicine, Wuhan 430065, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
SB  - IM
MH  - Animals
MH  - *Diabetes Mellitus, Experimental/genetics/therapy
MH  - *Diabetes Mellitus, Type 2
MH  - *Electroacupuncture
MH  - Glycogen Synthase Kinase 3 beta/genetics
MH  - Hippocampus
MH  - *Islets of Langerhans
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Diabetes
OT  - Electroacupuncture
OT  - Hippocampus
OT  - Pancreas
OT  - Tau phosphorylation
EDAT- 2021/12/06 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/12/05 21:59
PHST- 2021/12/05 21:59 [entrez]
PHST- 2021/12/06 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
AID - 10.13702/j.1000-0607.200921 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2021 Nov 25;46(11):901-6. doi: 10.13702/j.1000-0607.200921.