PMID- 34865326
OWN - NLM
STAT- MEDLINE
DCOM- 20211207
LR  - 20211214
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 46
IP  - 11
DP  - 2021 Nov 25
TI  - [Electroacupuncture improves glucose and lipid metabolism by regulating 
      APN/AMPK/PPARalpha signaling of skeletal muscle in Zucker diabetic obese rats].
PG  - 907-13
LID - 10.13702/j.1000-0607.20210201 [doi]
AB  - OBJECTIVE: To observe the effect of electroacupuncture (EA) on skeletal muscle 
      adiponectin receptor (Adipor1) / adenylate activated protein kinase (AMPK) / 
      peroxisome proliferator-activated receptor alpha (PPARalpha) signaling pathway and 
      skeletal muscle morphology by the secretion of serum adiponectin in Zucker 
      diabetic obese (ZDF) rats, so as to explore its mechanism underlying regulating 
      glucose and lipid metabolism of type 2 diabetes mellitus (T2DM) and improving 
      skeletal muscle insulin resistance (IR). METHODS: Twelve male ZDF rats and six 
      Zucker thin (ZL) rats were selected. The rats were fed with Purina#5008 high-fat 
      diet for four weeks to induce T2DM model after adaptive feeding with normal diet 
      for one week. The ZDF rats were randomly divided into model group and EA group 
      according to blood glucose level after modeling and 6 ZL rats were used as the 
      blank control group. Rats in the EA group were treated with "Pishu" (BL20), EA 
      stimulation of "Yishu" (EX-B3), "Zusanli" (ST36) and "Sanyinjiao" (SP6), once a 
      day and 6 times a week for 4 weeks. Rats of the model and blank control groups 
      were grabbed and fixed in the same way as EA group. Fasting blood glucose (FBG) 
      was measured before and after EA intervention. Serum levels of insulin (INS), 
      C-peptide (C-P), adiponectin (APN) were measured by radioimmunoassay, and those 
      of free fatty acid (FFA), low-density lipoprotein (LDL), cholesterol (TC), 
      triglyceride (TG) content determined by enzyme colorimetry and insulin resistance 
      index (HOMA-IR) was calculated. The protein expression levels of AdipoR1, AMPK 
      and PPARalpha proteins in the quadriceps femoris tissues were detected by Western 
      blot and histopathological changes of quadriceps femoris muscle were observed by 
      H.E. staining. RESULTS: Compared with the blank control group, the levels of FBG, 
      serum INS, C-P, FFA, LDL, TC, TG and HOMA-IR in the model group were 
      significantly increased (P<0.01, P<0.05), while the levels of serum APN and the 
      expressions of AdipoR1, AMPK and PPARalpha proteins in the skeletal muscle were 
      significantly decreased (P<0.01, P<0.05). Compared with the model group, the 
      levels of FBG, serum INS, C-P, FFA, LDL, TC and HOMA-IR in the EA group were 
      significantly decreased (P<0.01), and those of serum APN and expression levels of 
      AdipoR1, AMPK and PPARalpha proteins in the skeletal muscle significantly increased 
      (P<0.01), but the serum TG level had no remarkable change in the EA group 
      (P>0.05). In addition, H.E. staining showed disordered arrangement of skeletal 
      muscle cells, rupture and fuzziness of muscle fibers, enlargement of the space 
      between muscular fibers and infiltration of small number of adipose cells which 
      were relatively milder in the EA group. CONCLUSION: EA can reduce blood glucose 
      and lipid levels, and improve IR in ZDF rats, which may be related to its 
      functions in up-regulating AdipoR1/AMPK/PPARalpha signaling and in promoting APN 
      secretion.
FAU - Duan, Hao-Ru
AU  - Duan HR
AD  - School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing 102488, China.
FAU - Li, Rui
AU  - Li R
AD  - School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing 102488, China.
FAU - Song, Shan-Shan
AU  - Song SS
AD  - School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing 102488, China.
FAU - Hu, Su-Qin
AU  - Hu SQ
AD  - Department of Respirology and Allergy, the Third Affiliated Hospital of Shenzhen 
      University, Shenzhen 518020, Guangdong Province.
FAU - Zhuang, Shu-Ting
AU  - Zhuang ST
AD  - School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing 102488, China.
FAU - Li, Qiu-Yan
AU  - Li QY
AD  - School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing 102488, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (Adiponectin)
RN  - 0 (PPAR alpha)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Adiponectin/metabolism
MH  - Animals
MH  - *Diabetes Mellitus, Type 2/genetics/metabolism/therapy
MH  - *Electroacupuncture
MH  - Glucose/metabolism
MH  - Lipid Metabolism/genetics
MH  - Male
MH  - Muscle, Skeletal/metabolism
MH  - Obesity/genetics/metabolism/therapy
MH  - PPAR alpha/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Zucker
MH  - Signal Transduction
OTO - NOTNLM
OT  - Adiponectin signaling pathway
OT  - Disorder of glucose and lipid metabolism
OT  - Electroacupuncture
OT  - Insulin resistance
OT  - Type 2 diabetes
EDAT- 2021/12/06 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/12/05 21:59
PHST- 2021/12/05 21:59 [entrez]
PHST- 2021/12/06 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
AID - 10.13702/j.1000-0607.20210201 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2021 Nov 25;46(11):907-13. doi: 10.13702/j.1000-0607.20210201.