PMID- 34866170
OWN - NLM
STAT- MEDLINE
DCOM- 20211207
LR  - 20211214
IS  - 2589-0409 (Electronic)
IS  - 1110-0362 (Linking)
VI  - 33
IP  - 1
DP  - 2021 Dec 6
TI  - Prognostic significance of microRNA 17-92 cluster expression in Egyptian chronic 
      lymphocytic leukemia patients.
PG  - 37
LID - 10.1186/s43046-021-00097-x [doi]
AB  - BACKGROUND: Abnormal expression patterns of microRNAs (miRs) play an important 
      role in the development and progression of malignancy. Identification of the 
      clinical significance and prognostic value of these small molecules in chronic 
      lymphocytic leukemia (CLL); a disease of heterogeneous biological landscape and 
      clinical course, has always been of tremendous translational value. AIM: To 
      evaluate the prognostic value of microRNA17-92 cluster members in Egyptian CLL 
      patients. METHODS: The expression levels of miR17-92 cluster members were 
      evaluated by qRT-PCR, including miR17, miR18a, miR19a, miR19b-1, miR20a, and 
      miR92a-1. Other investigations included serum LDH, serum beta2 microglobulin (beta2M), 
      CD38 and ZAP70 expression by flow cytometry, fluorescence in situ hybridization 
      (FISH) for 17p deletion, and imaging studies (computerized tomography (CT) scans 
      of neck, chest, abdomen, and pelvis or PET-CT scans). RESULTS: Overexpression of 
      all members of the miRNA17-92 cluster was detected in CLL patients compared to 
      controls (p =  < 0.001 for all miRs while p = 0.01 for miR19b-1). A significant 
      positive correlation between Hb and miR17 and a significant negative correlation 
      between Hb and miR19b-1 were observed (p = 0.041, 0.017 respectively). A 
      statistically significant positive correlation between miR19b-1 expression and 
      each of the WBCs and absolute lymphocytic count (ALC) was detected (p = 0.023, 
      0.022 respectively). Moreover, a statistically significant relation between 
      miR19b-1 expression and advanced Binet stages was also found (p = 0.05). 
      Regarding miR18a, a statistically significant positive correlation with LDH level 
      was found (p = 0.003). We also found a significant positive correlation between 
      miR92a-1 and beta2M level (p = 0.005), as well as a significant relation between 
      miR17 and negative CD38 expression (p = 0.034). However, no significant 
      relationships between any of studied miRNA expression levels and 17p deletion or 
      response to treatment were observed. Patients who expressed miR19b-1 were 
      significantly indicated to start therapy at diagnosis (p = 0.05). The overall 
      survival of CLL patients included in our study was 90.2% after 1 year from the 
      time of diagnosis. Patients with high expression of miR19a had better OS than 
      those with low expression (p = 0.04). CONCLUSIONS: Overexpression of all members 
      of the miR17-92 cluster was detected in Egyptian CLL patients. MiR18a, miR19b-1, 
      and miR92a-1 also have an adverse prognostic value while miR17 can be considered 
      a good prognostic marker. High expression of miR19a is associated with better OS.
CI  - (c) 2021. The Author(s).
FAU - Khalifa, M M
AU  - Khalifa MM
AD  - Department of Internal Medicine, Hematology Unit, Faculty of Medicine, Alexandria 
      University, Alexandria, Egypt. marwakhalifa969@gmail.com.
FAU - Zaki, N E
AU  - Zaki NE
AD  - Department of Internal Medicine, Hematology Unit, Faculty of Medicine, Alexandria 
      University, Alexandria, Egypt.
FAU - Nazier, A A
AU  - Nazier AA
AD  - Department of Internal Medicine, Hematology Unit, Faculty of Medicine, Alexandria 
      University, Alexandria, Egypt.
FAU - Moussa, M A
AU  - Moussa MA
AD  - Department of Internal Medicine, Hematology Unit, Faculty of Medicine, Alexandria 
      University, Alexandria, Egypt.
FAU - Haleem, R Abdel
AU  - Haleem RA
AD  - Department of Clinical Pathology, Faculty of Medicine, Alexandria University, 
      Alexandria, Egypt.
FAU - Rabie, M A
AU  - Rabie MA
AD  - Department of Medical Laboratory Technology, Pharos University, Alexandria, 
      Egypt.
FAU - Mansour, A R
AU  - Mansour AR
AD  - Department of Clinical Pathology, Faculty of Medicine, Alexandria University, 
      Alexandria, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20211206
PL  - England
TA  - J Egypt Natl Canc Inst
JT  - Journal of the Egyptian National Cancer Institute
JID - 9424566
RN  - 0 (MIR17HG, human)
RN  - 0 (MIRN17 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Egypt/epidemiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis/genetics
MH  - *MicroRNAs/genetics
MH  - Positron Emission Tomography Computed Tomography
MH  - Prognosis
MH  - RNA, Long Noncoding
OTO - NOTNLM
OT  - Chronic lymphocytic leukemia (CLL)
OT  - MicroRNA
OT  - Prognosis
OT  - miRNA17-92 cluster
EDAT- 2021/12/07 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/12/06 06:03
PHST- 2021/09/06 00:00 [received]
PHST- 2021/11/14 00:00 [accepted]
PHST- 2021/12/06 06:03 [entrez]
PHST- 2021/12/07 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
AID - 10.1186/s43046-021-00097-x [pii]
AID - 10.1186/s43046-021-00097-x [doi]
PST - epublish
SO  - J Egypt Natl Canc Inst. 2021 Dec 6;33(1):37. doi: 10.1186/s43046-021-00097-x.