PMID- 34866976
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211207
IS  - 1319-562X (Print)
IS  - 2213-7106 (Electronic)
IS  - 2213-7106 (Linking)
VI  - 28
IP  - 12
DP  - 2021 Dec
TI  - Isolation and molecular characterization of causal agent of blue mold on Allium 
      cepa L. and its control by Pennisetum flaccidum Griseb.
PG  - 6774-6781
LID - 10.1016/j.sjbs.2021.07.050 [doi]
AB  - Blue mold pathogen, isolated from infected Allium cepa L., was identified as a 
      Penicillium species through morphological and molecular characterisation. 
      Internal Transcribed spacer (ITS) region of ribosomal DNA (rDNA) was utilised for 
      DNA sequencing. Basic Local Alignment Search Tool (BLAST) analysis has found the 
      maximum similarity index of the fungus to be 82.39% with the Uncultured 
      Penicillium clone (Accession: MF535522). So, the isolated Penicillium specie is 
      the first reported specie of the genus that infects onion. A phylogenetic tree 
      was constructed to establish a relationship of the isolated fungus with the most 
      relevant species reported on GenBank. Extracts of Pennisetum flaccidum Griseb. 
      were evaluated against the isolated fungus as a potential biocontrol agent. Among 
      the five tested methanol concentrations (0.5%, 1%, 1.5%, 2% and 2.5%) of each 
      plant part (root, inflorescence and foliage), 0.5% root extract showed maximum 
      growth retardation, i.e. 89%. For bioassay-guided fractionation, the root extract 
      was partitioned in n-hexane, chloroform, n-butanol and ethyl acetate. Ethyl 
      acetate (1%) was proved to be the most potent one. Phytochemical screening has 
      confirmed the occurrence of terpenoids, tannins, saponins and alkaloids. The 
      applied molecular approach has deduced that the Penicillium specie collected from 
      Pakistan might be novel. This study can be concluded that P. flaccidum contains 
      potent phytochemicals which might be used as antifungal agent against Penicillium 
      species.
CI  - (c) 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University.
FAU - Zubairi, Tehzeeb
AU  - Zubairi T
AD  - Department of Botany, Lahore College for Women University, Lahore 54000, 
      Pakistan.
FAU - Jabeen, Khajista
AU  - Jabeen K
AD  - Department of Botany, Lahore College for Women University, Lahore 54000, 
      Pakistan.
FAU - Khalid, Sana
AU  - Khalid S
AD  - Department of Botany, Lahore College for Women University, Lahore 54000, 
      Pakistan.
AD  - Institute of Agricultural Sciences, University of the Punjab, Lahore 54000, 
      Pakistan.
FAU - Iqbal, Sumera
AU  - Iqbal S
AD  - Department of Botany, Lahore College for Women University, Lahore 54000, 
      Pakistan.
LA  - eng
PT  - Journal Article
DEP - 20210724
PL  - Saudi Arabia
TA  - Saudi J Biol Sci
JT  - Saudi journal of biological sciences
JID - 101543796
PMC - PMC8626295
OTO - NOTNLM
OT  - Biocontrol
OT  - Microscopic identification
OT  - Pathogenicity test
OT  - Penicillium specie
OT  - Phytochemical screening
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2021/12/07 06:00
MHDA- 2021/12/07 06:01
PMCR- 2021/07/24
CRDT- 2021/12/06 09:01
PHST- 2020/05/13 00:00 [received]
PHST- 2021/07/13 00:00 [revised]
PHST- 2021/07/16 00:00 [accepted]
PHST- 2021/12/06 09:01 [entrez]
PHST- 2021/12/07 06:00 [pubmed]
PHST- 2021/12/07 06:01 [medline]
PHST- 2021/07/24 00:00 [pmc-release]
AID - S1319-562X(21)00633-1 [pii]
AID - 10.1016/j.sjbs.2021.07.050 [doi]
PST - ppublish
SO  - Saudi J Biol Sci. 2021 Dec;28(12):6774-6781. doi: 10.1016/j.sjbs.2021.07.050. 
      Epub 2021 Jul 24.