PMID- 3486824
OWN - NLM
STAT- MEDLINE
DCOM- 19860718
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 58
IP  - 2
DP  - 1986 Jun
TI  - Production of human and murine interleukin-2 by toxic shock syndrome toxin-1.
PG  - 203-8
AB  - Toxic shock syndrome toxin-1 (TSST-1), isolated from Staphylococcus aureus 
      strains associated with toxic shock syndrome (TSS), is known as a potent mitogen 
      and interleukin-1 inducer. The potential of TSST-1 as an interleukin-2 (IL-2) 
      inducer was tested on human peripheral blood lymphocytes (HPBL) and murine spleen 
      lymphocytes (MSL). These cells were incubated with TSST-1 and the supernatants 
      analysed for IL-2 production. Preincubation of IL-2-dependent indicator cells 
      (IC) with a monoclonal antibody specific for murine IL-2 receptors inhibited 
      their proliferation by supernatants of TSST-1-treated MSL, thus strongly 
      suggesting that they contain IL-2. The concentrations of TSST-1 required for HPBL 
      or MSL to produce IL-2 ranged between 10(-1) and 10(-4) micrograms/ml. The amount 
      of IL-2 units/ml varied little from one experiment to another. In contrast, IL-2 
      production by PHA-stimulated HPBL or Con A-stimulated MSL showed great 
      variability and dependence on mitogen concentration. T-cell depleted MSL exposed 
      to TSST-1 produced less IL-2. Experiments with germ-free mice and TSST-1-primed 
      mice demonstrated that IL-2 production is not related to TSST-1 antigenicity.
FAU - Micusan, V V
AU  - Micusan VV
FAU - Mercier, G
AU  - Mercier G
FAU - Bhatti, A R
AU  - Bhatti AR
FAU - Reiser, R F
AU  - Reiser RF
FAU - Bergdoll, M S
AU  - Bergdoll MS
FAU - Oth, D
AU  - Oth D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antilymphocyte Serum)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Interleukin-2)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Superantigens)
RN  - 0 (enterotoxin F, Staphylococcal)
RN  - 11028-71-0 (Concanavalin A)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antilymphocyte Serum
MH  - Bacterial Toxins/*pharmacology
MH  - Concanavalin A
MH  - Dose-Response Relationship, Drug
MH  - Enterotoxins/*pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-2/*biosynthesis
MH  - Lymphocyte Activation
MH  - Lymphocytes/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitosis/drug effects
MH  - Phytohemagglutinins
MH  - Shock, Septic/*immunology
MH  - Spleen/immunology
MH  - Staphylococcus aureus
MH  - *Superantigens
MH  - T-Lymphocytes/immunology
PMC - PMC1452667
EDAT- 1986/06/01 00:00
MHDA- 1986/06/01 00:01
PMCR- 1987/06/01
CRDT- 1986/06/01 00:00
PHST- 1986/06/01 00:00 [pubmed]
PHST- 1986/06/01 00:01 [medline]
PHST- 1986/06/01 00:00 [entrez]
PHST- 1987/06/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1986 Jun;58(2):203-8.