PMID- 34870754
OWN - NLM
STAT- MEDLINE
DCOM- 20220524
LR  - 20220524
IS  - 1573-0832 (Electronic)
IS  - 0301-486X (Linking)
VI  - 187
IP  - 2-3
DP  - 2022 Jun
TI  - PbGP43 Genotyping Using Paraffin-Embedded Biopsies of Human 
      Paracoccidioidomycosis Reveals a Genetically Distinct Lineage in the 
      Paracoccidioides brasiliensis Complex.
PG  - 157-168
LID - 10.1007/s11046-021-00608-3 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic mycosis caused by a group of cryptic 
      species embedded in the Paracoccidioides brasiliensis complex and 
      Paracoccidioides lutzii. Four species were recently inferred to belong to the P. 
      brasiliensis complex, but the high genetic diversity found in both human and 
      environmental samples have suggested that the number of lineages may be higher. 
      This study aimed to assess the 43-kilodalton glycoprotein genotypes (PbGP43) in 
      paraffin-embedded samples from PCM patients to infer the phylogenetic lineages of 
      the P. brasiliensis complex responsible for causing the infection. 
      Formalin-fixed, paraffin-embedded (FFPE) tissue samples from patients with 
      histopathological diagnosis of PCM were analyzed. DNAs were extracted and 
      amplified for a region of the second exon of the PbGP43 gene. Products were 
      sequenced and aligned with other PbGP43 sequences available. A haplotype network 
      and the phylogenetic relationships among sequences were inferred. Amino acid 
      substitutions were investigated regarding the potential to modify physicochemical 
      properties in the proteins. Six phylogenetic lineages were identified as 
      belonging to the P. brasiliensis complex. Two lineages did not group with any of 
      the four recognized species of the complex, and, interestingly, one of them 
      comprised only FFPE samples. A coinfection involving two lineages was found. Five 
      parsimony-informative sites were identified and three of them showed radical 
      non-synonymous substitutions with the potential to promote changes in the 
      protein. This study expands the knowledge regarding the genetic diversity 
      existing in the P. brasiliensis complex and shows the potential of FFPE samples 
      in species identification and in detecting coinfections.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Ricci, Giannina
AU  - Ricci G
AD  - Centro de Diagnostico e Pesquisa em Biologia Molecular Dr Ivo Ricci, Sao Carlos, 
      SP, Brazil. gianninaricci@uol.com.br.
AD  - Departamento de Patologia, Escola Paulista de Medicina, Universidade Federal de 
      Sao Paulo, Sao Paulo, SP, Brazil. gianninaricci@uol.com.br.
FAU - Campanini, Emeline Boni
AU  - Campanini EB
AD  - Departamento de Genetica e Evolucao, Universidade Federal de Sao Carlos, Sao 
      Carlos, SP, Brazil.
FAU - Nishikaku, Angela Satie
AU  - Nishikaku AS
AD  - Centro de Diagnostico e Pesquisa em Biologia Molecular Dr Ivo Ricci, Sao Carlos, 
      SP, Brazil.
FAU - Puccia, Rosana
AU  - Puccia R
AD  - Disciplina de Biologia Celular, Departamento de Microbiologia, Imunologia e 
      Parasitologia, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Marques, Mariangela
AU  - Marques M
AD  - Faculdade de Medicina de Botucatu, Universidade Estadual Paulista Julio de 
      Mesquita Filho (UNESP), Botucatu, SP, Brazil.
FAU - Bialek, Ralf
AU  - Bialek R
AD  - LADR GmbH MVZ Dr, Kramer & Kollegen, Lauenburger Strasse 67, 21502, Geesthacht, 
      Germany.
FAU - Rodrigues, Anderson Messias
AU  - Rodrigues AM
AD  - Disciplina de Biologia Celular, Departamento de Microbiologia, Imunologia e 
      Parasitologia, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Batista, Wagner Luiz
AU  - Batista WL
AD  - Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo, Campus 
      Diadema, Sao Paulo, SP, Brazil.
LA  - eng
GR  - 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/
GR  - 313375/2017-8/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
GR  - 06-05095-6/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
PT  - Journal Article
DEP - 20211206
PL  - Netherlands
TA  - Mycopathologia
JT  - Mycopathologia
JID - 7505689
RN  - 0 (Antigens, Fungal)
RN  - 0 (Fungal Proteins)
SB  - IM
MH  - Antigens, Fungal/genetics
MH  - Biopsy
MH  - Fungal Proteins/genetics
MH  - Genotype
MH  - Humans
MH  - *Paracoccidioides/genetics
MH  - *Paracoccidioidomycosis/diagnosis
MH  - Paraffin Embedding
MH  - Phylogeny
OTO - NOTNLM
OT  - Formalin-fixed paraffin-embedded (FFPE) biopsies
OT  - Molecular diagnosis
OT  - PCR
OT  - Paracoccidioides brasiliensis complex
OT  - PbGP43 genotyping
EDAT- 2021/12/07 06:00
MHDA- 2022/05/25 06:00
CRDT- 2021/12/06 12:28
PHST- 2021/08/29 00:00 [received]
PHST- 2021/11/12 00:00 [accepted]
PHST- 2021/12/07 06:00 [pubmed]
PHST- 2022/05/25 06:00 [medline]
PHST- 2021/12/06 12:28 [entrez]
AID - 10.1007/s11046-021-00608-3 [pii]
AID - 10.1007/s11046-021-00608-3 [doi]
PST - ppublish
SO  - Mycopathologia. 2022 Jun;187(2-3):157-168. doi: 10.1007/s11046-021-00608-3. Epub 
      2021 Dec 6.