PMID- 34873729
OWN - NLM
STAT- MEDLINE
DCOM- 20220419
LR  - 20220509
IS  - 1096-9071 (Electronic)
IS  - 0146-6615 (Linking)
VI  - 94
IP  - 6
DP  - 2022 Jun
TI  - Identification of an IRES within the coding region of the structural protein of 
      human rhinovirus 16.
PG  - 2653-2661
LID - 10.1002/jmv.27507 [doi]
AB  - As an alternative mechanism for cap-dependent (m7GpppN) translation, internal 
      ribosome entry site (IRES)-dependent translation has been observed in the 5' 
      untranslated regions (5' UTR) and coding regions of a number of viral and 
      eukaryotic mRNAs. In this study, a series of 5' terminal truncated structural 
      protein genes that were fused with GFP was used to screen for potential IRESs, 
      and IRESs were identified using a bicistronic luciferase vector or GFP expression 
      vector possessing a hairpin structure. Our results revealed that a putative IRES 
      was located between nt 1982 and 2281 in the VP3 coding region of the human 
      rhinovirus 16 (HRV16) genomes. We also demonstrated that effective IRES-initiated 
      protein expression in vitro did not occur through splicing sites or cryptic 
      promoters. We confirmed that thapsigargin (TG), an inducer of endoplasmic 
      reticulum stress (ERS), facilitated increased IRES activity in a dose-dependent 
      manner. Additionally, the secondary structure of the IRES was predicted online 
      using the RNAfold web server.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Shi, Bingtian
AU  - Shi B
AUID- ORCID: 0000-0001-7411-9547
AD  - Center for Viral Resource, State Key Laboratory of Infectious Disease Prevention 
      and Control, National Institute for Viral Disease Control and Prevention, Chinese 
      Center for Disease Control and Prevention, Beijing, China.
AD  - Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
FAU - Song, Qinqin
AU  - Song Q
AUID- ORCID: 0000-0003-1418-4763
AD  - Center for Viral Resource, State Key Laboratory of Infectious Disease Prevention 
      and Control, National Institute for Viral Disease Control and Prevention, Chinese 
      Center for Disease Control and Prevention, Beijing, China.
AD  - Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
FAU - Luo, Xiaonuan
AU  - Luo X
AUID- ORCID: 0000-0003-3743-5481
AD  - Department of Pathology, School of Basic Medical Sciences, Shenzhen University, 
      Shenzhen, Guangdong, China.
FAU - Song, Juan
AU  - Song J
AUID- ORCID: 0000-0002-5471-5031
AD  - Center for Viral Resource, State Key Laboratory of Infectious Disease Prevention 
      and Control, National Institute for Viral Disease Control and Prevention, Chinese 
      Center for Disease Control and Prevention, Beijing, China.
AD  - Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
FAU - Xia, Dong
AU  - Xia D
AUID- ORCID: 0000-0003-1320-7177
AD  - Center for Viral Resource, State Key Laboratory of Infectious Disease Prevention 
      and Control, National Institute for Viral Disease Control and Prevention, Chinese 
      Center for Disease Control and Prevention, Beijing, China.
AD  - Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
FAU - Xia, Zhiqiang
AU  - Xia Z
AUID- ORCID: 0000-0003-0251-7153
AD  - Center for Viral Resource, State Key Laboratory of Infectious Disease Prevention 
      and Control, National Institute for Viral Disease Control and Prevention, Chinese 
      Center for Disease Control and Prevention, Beijing, China.
AD  - Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
FAU - Liu, Mi
AU  - Liu M
AUID- ORCID: 0000-0002-6508-0115
AD  - Center for Viral Resource, State Key Laboratory of Infectious Disease Prevention 
      and Control, National Institute for Viral Disease Control and Prevention, Chinese 
      Center for Disease Control and Prevention, Beijing, China.
AD  - Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
FAU - Wang, Wenjun
AU  - Wang W
AUID- ORCID: 0000-0003-4934-3007
AD  - Center for Viral Resource, State Key Laboratory of Infectious Disease Prevention 
      and Control, National Institute for Viral Disease Control and Prevention, Chinese 
      Center for Disease Control and Prevention, Beijing, China.
AD  - Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
FAU - Wang, Ruifang
AU  - Wang R
AUID- ORCID: 0000-0002-3849-9914
AD  - Center for Viral Resource, State Key Laboratory of Infectious Disease Prevention 
      and Control, National Institute for Viral Disease Control and Prevention, Chinese 
      Center for Disease Control and Prevention, Beijing, China.
AD  - Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
FAU - Du, Haijun
AU  - Du H
AUID- ORCID: 0000-0001-5425-5488
AD  - Center for Viral Resource, State Key Laboratory of Infectious Disease Prevention 
      and Control, National Institute for Viral Disease Control and Prevention, Chinese 
      Center for Disease Control and Prevention, Beijing, China.
AD  - Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
FAU - Wei, Qiang
AU  - Wei Q
AUID- ORCID: 0000-0001-8914-1069
AD  - National Pathogen Resource Center, Chinese Center for Disease Control and 
      Prevention, Beijing, China.
FAU - Han, Jun
AU  - Han J
AUID- ORCID: 0000-0002-0803-4403
AD  - Center for Viral Resource, State Key Laboratory of Infectious Disease Prevention 
      and Control, National Institute for Viral Disease Control and Prevention, Chinese 
      Center for Disease Control and Prevention, Beijing, China.
AD  - Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211215
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Internal Ribosome Entry Sites)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Humans
MH  - *Internal Ribosome Entry Sites/genetics
MH  - Protein Biosynthesis
MH  - *Rhinovirus/genetics
MH  - Ribosomes/genetics/metabolism
OTO - NOTNLM
OT  - Human rhinovirus 16
OT  - VP3 gene
OT  - coding region
OT  - endoplasmic reticulum stress
OT  - internal ribosome entry sites (IRESs)
EDAT- 2021/12/08 06:00
MHDA- 2022/04/20 06:00
CRDT- 2021/12/07 06:53
PHST- 2021/11/13 00:00 [revised]
PHST- 2021/06/11 00:00 [received]
PHST- 2021/12/02 00:00 [accepted]
PHST- 2021/12/08 06:00 [pubmed]
PHST- 2022/04/20 06:00 [medline]
PHST- 2021/12/07 06:53 [entrez]
AID - 10.1002/jmv.27507 [doi]
PST - ppublish
SO  - J Med Virol. 2022 Jun;94(6):2653-2661. doi: 10.1002/jmv.27507. Epub 2021 Dec 15.