PMID- 34875430 OWN - NLM STAT- MEDLINE DCOM- 20220112 LR - 20220112 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 374 DP - 2022 Apr 16 TI - Click reaction triggered turn-on fluorescence strategy for highly sensitive and selective determination of steroid hormones in food samples. PG - 131565 LID - S0308-8146(21)02571-1 [pii] LID - 10.1016/j.foodchem.2021.131565 [doi] AB - The copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction has becoming noticeable in the field of analytical chemistry. Mild reaction conditions, simple operation, high efficiency, and good regioselectivity make this classical click reaction a perfect strategy for chemical derivatization. Herein, we proposed a promising click fluorescent labeling method with high selectivity for the determination of five steroid hormones in food samples. The labeling strategy depends on the reaction between 3-Azido-7-hydroxycoumarin and the alkynyl group of steroid hormones, which shows a turn-on fluorescence response in the presence of copper (I). The formed fluorescent products were detected by HPLC-FLD. Under the optimized conditions, the proposed method presented excellent performance with good linearity (R(2) >/= 0.9998) and low detection limit (1.8-7.3 mug L(-1)). Further, satisfactory recoveries were obtained to be 82-107% in spiked meats with relative standard deviations (RSDs)