PMID- 3487788
OWN - NLM
STAT- MEDLINE
DCOM- 19860820
LR  - 20201216
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 83
IP  - 14
DP  - 1986 Jul
TI  - Tumor necrosis factor: a potent effector molecule for tumor cell killing by 
      activated macrophages.
PG  - 5233-7
AB  - Activated macrophages (aM phi) destroy more effectively cancer cells than normal 
      cells. The mechanism by which macrophages destroy cancer cells is not known. We 
      report here that tumor cells susceptible to aM phi were killed by recombinant (r) 
      tumor necrosis factor type alpha (TNF-alpha), whereas variant tumor cells 
      resistant to aM phi after selection in vitro or in vivo were resistant to killing 
      by rTNF-alpha. The converse selection for rTNF-alpha-resistant variants resulted 
      in cells that were also resistant to killing by aM phi. The sensitivity of 
      macrophage-resistant variants was not changed to other tumoricidal cells or 
      soluble mediators, except that the macrophage-resistant variants were also 
      resistant to the effects of another cytotoxic protein, B-cell lymphotoxin, which 
      is structurally related to rTNF-alpha. Similar results were obtained regardless 
      of whether short-term or long-term cytotoxic effects of aM phi were measured. 
      Finally, it was shown that killing of tumor cells by murine aM phi was completely 
      inhibited with a polyclonal antibody that neutralizes the effects of murine 
      TNF-alpha. These results suggest a major role for TNF-alpha in tumor cell 
      destruction by aM phi in vitro and in vivo.
FAU - Urban, J L
AU  - Urban JL
FAU - Shepard, H M
AU  - Shepard HM
FAU - Rothstein, J L
AU  - Rothstein JL
FAU - Sugarman, B J
AU  - Sugarman BJ
FAU - Schreiber, H
AU  - Schreiber H
LA  - eng
GR  - CA-19266/CA/NCI NIH HHS/United States
GR  - CA-22677/CA/NCI NIH HHS/United States
GR  - CA-37156/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Glycoproteins)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Cytotoxicity, Immunologic/drug effects
MH  - Drug Resistance
MH  - Glycoproteins/pharmacology/*therapeutic use
MH  - Humans
MH  - Lymphotoxin-alpha/pharmacology
MH  - *Macrophage Activation
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Neoplasms, Experimental/*drug therapy
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Necrosis Factor-alpha
PMC - PMC323925
EDAT- 1986/07/01 00:00
MHDA- 1986/07/01 00:01
PMCR- 1987/01/01
CRDT- 1986/07/01 00:00
PHST- 1986/07/01 00:00 [pubmed]
PHST- 1986/07/01 00:01 [medline]
PHST- 1986/07/01 00:00 [entrez]
PHST- 1987/01/01 00:00 [pmc-release]
AID - 10.1073/pnas.83.14.5233 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1986 Jul;83(14):5233-7. doi: 10.1073/pnas.83.14.5233.