PMID- 34880327
OWN - NLM
STAT- MEDLINE
DCOM- 20220128
LR  - 20230921
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Dec 8
TI  - Interactions between Babesia microti merozoites and rat kidney cells in a 
      short-term in vitro culture and animal model.
PG  - 23663
LID - 10.1038/s41598-021-03079-0 [doi]
LID - 23663
AB  - Babesiosis is one of the most common infections in free-living animals and is 
      rapidly becoming significant among human zoonoses. Cases of acute renal failure 
      in humans caused by Babesia spp. have been described in the literature. The 
      kidneys are characterised by intense blood flow through the blood vessels, which 
      increases the likelihood of contact with the intra-erythrocyte parasite. The aim 
      of this study was to observe the influence of B. microti (ATCC 30221) on renal 
      epithelial cells in vitro cultured (NRK-52E line) and Wistar rats' kidney. Both 
      NRK-52E cells and rats' kidney sections were analysed by light microscopy, 
      transmission electron microscopy (TEM) and fluorescence in situ hybridization 
      (FISH). Necrotic changes in renal epithelial cells have been observed in vitro 
      and in vivo. In many cross-sections through the rats' kidney, adhesion of blood 
      cells to the vascular endothelium, accumulation of erythrocytes and emboli were 
      demonstrated. In NRK-52E culture, elements with a distinctly doubled cell 
      membrane resembling B. microti were found inside the cytoplasm and adjacent to 
      the cell layer. The study indicates a chemotactic tendency for B. microti to 
      adhere to the renal tubules' epithelium, a possibility of piroplasms entering the 
      renal epithelial cells, their proliferation within the cytoplasm and emboli 
      formation.
CI  - (c) 2021. The Author(s).
FAU - Albertynska, Marta
AU  - Albertynska M
AD  - Department of Pathology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical 
      University of Silesia in Katowice, Ostrogorska 30, 41-200, Sosnowiec, Poland.
AD  - Provincial Sanitary and Epidemiological Station in Katowice, Raciborska 39, 
      40-074, Katowice, Poland.
FAU - Okla, Hubert
AU  - Okla H
AD  - Provincial Sanitary and Epidemiological Station in Katowice, Raciborska 39, 
      40-074, Katowice, Poland. hubert.okla@gmail.com.
AD  - Faculty of Science and Technology, University of Silesia in Katowice, 75 Pulku 
      Piechoty 1, 41-500, Chorzow, Poland. hubert.okla@gmail.com.
FAU - Jasik, Krzysztof
AU  - Jasik K
AD  - Department of Pathology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical 
      University of Silesia in Katowice, Ostrogorska 30, 41-200, Sosnowiec, Poland. 
      kjasik@sum.edu.pl.
FAU - Urbanska-Jasik, Danuta
AU  - Urbanska-Jasik D
AD  - Institute of Biology, Biotechnology and Environmental Protection, Faculty of 
      Natural Sciences, University of Silesia in Katowice, Bankowa 9, 40-007, Katowice, 
      Poland.
FAU - Pol, Przemyslaw
AU  - Pol P
AD  - Department of Small Livestock Breeding, The National Research Institute of Animal 
      in Krakow, Krakowska 1, 32-083, Balice, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211208
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Animals
MH  - Babesia microti/*physiology
MH  - Babesiosis/parasitology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Epithelial Cells/*metabolism/ultrastructure
MH  - Erythrocytes/parasitology/ultrastructure
MH  - *Host-Parasite Interactions
MH  - Kidney Tubules/*cytology
MH  - Merozoites/*physiology
MH  - Rats
PMC - PMC8654915
COIS- The authors declare no competing interests.
EDAT- 2021/12/10 06:00
MHDA- 2022/01/29 06:00
PMCR- 2021/12/08
CRDT- 2021/12/09 06:25
PHST- 2021/04/26 00:00 [received]
PHST- 2021/11/22 00:00 [accepted]
PHST- 2021/12/09 06:25 [entrez]
PHST- 2021/12/10 06:00 [pubmed]
PHST- 2022/01/29 06:00 [medline]
PHST- 2021/12/08 00:00 [pmc-release]
AID - 10.1038/s41598-021-03079-0 [pii]
AID - 3079 [pii]
AID - 10.1038/s41598-021-03079-0 [doi]
PST - epublish
SO  - Sci Rep. 2021 Dec 8;11(1):23663. doi: 10.1038/s41598-021-03079-0.