PMID- 34880869
OWN - NLM
STAT- MEDLINE
DCOM- 20220210
LR  - 20220210
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 12
DP  - 2021
TI  - Conditioned Medium of Human Amniotic Epithelial Cells Alleviates Experimental 
      Allergic Conjunctivitis Mainly by IL-1ra and IL-10.
PG  - 774601
LID - 10.3389/fimmu.2021.774601 [doi]
LID - 774601
AB  - Allergic conjunctivitis (AC) is the most prevalent form of mucosal allergy, and 
      the conditioned medium (CM) from mesenchymal stem cells has been reported to 
      attenuate some allergic diseases. However, the therapeutic effects of CM from 
      different tissue stem cells (TSC-CM) on allergic diseases have not been tested. 
      Here, we studied the effects of topical administration of different human TSC-CM 
      on experimental AC (EAC) mice. Only human amniotic epithelial cell-CM (AECM) 
      significantly attenuated allergic eye symptoms and reduced the infiltration of 
      immune cells and the levels of local inflammatory factors in the conjunctiva 
      compared to EAC mice. In addition, AECM treatment decreased immunoglobulin E 
      (IgE) release, histamine production, and the hyperpermeability of conjunctival 
      vessels. Protein chip assays revealed that the levels of anti-inflammatory 
      factors, interleukin-1 receptor antagonist (IL-1ra) and IL-10, were higher in 
      AECM compared to other TSC-CM. Furthermore, the anti-allergic effects of AECM on 
      EAC mice were abrogated when neutralized with IL-1ra or IL-10 antibody, and the 
      similar phenomenon was for the activation and function of B cells and mast cells. 
      Together, the present study demonstrated that AECM alleviates EAC symptoms by 
      multiple anti-allergic mechanisms mainly via IL-1ra and IL-10. Such topical AECM 
      therapy may represent a novel and feasible strategy for treating AC.
CI  - Copyright (c) 2021 Wu, Gao, Lin, Lu, Xu and Xu.
FAU - Wu, Binxin
AU  - Wu B
AD  - Department of Ophthalmology of Shanghai Tenth People's Hospital, Laboratory of 
      Clinical Visual Science of Tongji Eye Institute, Tongji University School of 
      Medicine, Shanghai, China.
FAU - Gao, Furong
AU  - Gao F
AD  - Department of Ophthalmology of Shanghai Tenth People's Hospital, Laboratory of 
      Clinical Visual Science of Tongji Eye Institute, Tongji University School of 
      Medicine, Shanghai, China.
FAU - Lin, Jianhua
AU  - Lin J
AD  - Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai, China.
FAU - Lu, Lixia
AU  - Lu L
AD  - Department of Ophthalmology of Shanghai Tenth People's Hospital, Laboratory of 
      Clinical Visual Science of Tongji Eye Institute, Tongji University School of 
      Medicine, Shanghai, China.
FAU - Xu, Huiming
AU  - Xu H
AD  - State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Clinical Stem 
      Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong 
      University, Shanghai, China.
FAU - Xu, Guo-Tong
AU  - Xu GT
AD  - Department of Ophthalmology of Shanghai Tenth People's Hospital, Laboratory of 
      Clinical Visual Science of Tongji Eye Institute, Tongji University School of 
      Medicine, Shanghai, China.
AD  - Department of Pharmacology, Tongji University School of Medicine, Shanghai, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211122
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adipogenesis
MH  - Amnion/*cytology
MH  - Animals
MH  - B-Lymphocytes/immunology/metabolism
MH  - Capillary Permeability
MH  - Cells, Cultured
MH  - Conjunctivitis, Allergic/diagnosis/*etiology/*metabolism
MH  - Culture Media, Conditioned/*pharmacology
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Disease Susceptibility
MH  - Epithelial Cells/*metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-10/*metabolism
MH  - Interleukin-1alpha/*metabolism
MH  - Mice
MH  - Osteogenesis
MH  - Pregnancy
PMC - PMC8645696
OTO - NOTNLM
OT  - AECM
OT  - EAC
OT  - IL-10
OT  - IL-1ra
OT  - tissue stem cells
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/12/10 06:00
MHDA- 2022/02/11 06:00
PMCR- 2021/01/01
CRDT- 2021/12/09 07:00
PHST- 2021/09/12 00:00 [received]
PHST- 2021/10/25 00:00 [accepted]
PHST- 2021/12/09 07:00 [entrez]
PHST- 2021/12/10 06:00 [pubmed]
PHST- 2022/02/11 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2021.774601 [doi]
PST - epublish
SO  - Front Immunol. 2021 Nov 22;12:774601. doi: 10.3389/fimmu.2021.774601. eCollection 
      2021.