PMID- 34881166
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240404
IS  - 2190-572X (Print)
IS  - 2190-5738 (Electronic)
IS  - 2190-5738 (Linking)
VI  - 11
IP  - 12
DP  - 2021 Dec
TI  - Identification of the key functional genes in salt-stress tolerance of 
      Cyanobacterium Phormidium tenue using in silico analysis.
PG  - 503
LID - 10.1007/s13205-021-03050-w [doi]
LID - 503
AB  - The development of artificial biocrust using cyanobacterium Phormidium tenue has 
      been suggested as an effective strategy to prevent soil degradation. Here, a 
      combination of in silico approaches with growth rate, photosynthetic pigment, 
      morphology, and transcript analysis was used to identify specific genes and their 
      protein products in response to 500 mM NaCl in P. tenue. The results show that 
      500 mM NaCl induces the expression of genes encoding glycerol-3-phosphate 
      dehydrogenase (glpD) as a Flavoprotein, ribosomal protein S12 
      methylthiotransferase (rimO), and a hypothetical protein (sll0939). The 
      constructed co-expression network revealed a group of abiotic stress-responsive 
      genes. Using the Basic Local Alignment Search Tool (BLAST), the homologous 
      proteins of rimO, glpD, and sll0939 were identified in the P. tenue genome. 
      Encoded proteins of glpD, rimO, and DUF1622 genes, respectively, contain (DAO and 
      DAO C), (UPF0004, Radical SAM and TRAM 2), and (DUF1622) domains. The predicted 
      ligand included 22B and MG for DUF1622, FS5 for rimO, and FAD for glpD protein. 
      There was no direct disruption in ligand-binding sites of these proteins by 
      Na(+), Cl(-), or NaCl. The growth rate, photosynthetic pigment, and morphology of 
      P. tenue were investigated, and the result showed an acceptable tolerance rate of 
      this microorganism under salt stress. The quantitative real-time polymerase chain 
      reaction (qRT-PCR) results revealed the up-regulation of glpD, rimO, and DUF1622 
      genes under salt stress. This is the first report on computational and 
      experimental analyses of the glpD, rimO, and DUF1622 genes in P. tenue under salt 
      stress to the best of our knowledge. SUPPLEMENTARY INFORMATION: The online 
      version contains supplementary material available at 10.1007/s13205-021-03050-w.
CI  - (c) King Abdulaziz City for Science and Technology 2021.
FAU - Shahbazi, Mehrdad
AU  - Shahbazi M
AD  - Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, 
      1983969411 Tehran, Iran. GRID: grid.412502.0. ISNI: 0000 0001 0686 4748
FAU - Tohidfar, Masoud
AU  - Tohidfar M
AUID- ORCID: 0000-0002-0175-7406
AD  - Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, 
      1983969411 Tehran, Iran. GRID: grid.412502.0. ISNI: 0000 0001 0686 4748
FAU - Azimzadeh Irani, Maryam
AU  - Azimzadeh Irani M
AD  - Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, 
      1983969411 Tehran, Iran. GRID: grid.412502.0. ISNI: 0000 0001 0686 4748
LA  - eng
PT  - Journal Article
DEP - 20211118
PL  - Germany
TA  - 3 Biotech
JT  - 3 Biotech
JID - 101565857
PMC - PMC8602552
OTO - NOTNLM
OT  - Biocrust
OT  - Homology modeling
OT  - Molecular docking
OT  - NaCl
OT  - Phormidium tenue
OT  - Transcript analysis
EDAT- 2021/12/10 06:00
MHDA- 2021/12/10 06:01
PMCR- 2022/12/01
CRDT- 2021/12/09 07:03
PHST- 2021/07/16 00:00 [received]
PHST- 2021/10/31 00:00 [accepted]
PHST- 2021/12/09 07:03 [entrez]
PHST- 2021/12/10 06:00 [pubmed]
PHST- 2021/12/10 06:01 [medline]
PHST- 2022/12/01 00:00 [pmc-release]
AID - 3050 [pii]
AID - 10.1007/s13205-021-03050-w [doi]
PST - ppublish
SO  - 3 Biotech. 2021 Dec;11(12):503. doi: 10.1007/s13205-021-03050-w. Epub 2021 Nov 
      18.