PMID- 34884536
OWN - NLM
STAT- MEDLINE
DCOM- 20211220
LR  - 20211220
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 23
DP  - 2021 Nov 25
TI  - Investigation of H(2)S Donor Treatment on Neutrophil Extracellular Traps in 
      Experimental Colitis.
LID - 10.3390/ijms222312729 [doi]
LID - 12729
AB  - Inflammatory bowel diseases (IBD) are chronic, immune-mediated disorders, which 
      affect the gastrointestinal tract with intermittent ulceration. It is 
      increasingly clear that neutrophil extracellular traps (NETs) seem to have a role 
      in IBD; however, the associated pathogenesis is still not known. Furthermore, 
      several conventional therapies are available against IBD, although these might 
      have side effects. Our current study aimed to investigate the effects of hydrogen 
      sulfide (H(2)S) treatment on NETs formation and on the expression of inflammatory 
      mediators in experimental rat colitis. To model IBD, 
      2,4,6-trinitrobenzenesulfonic acid (TNBS) was administered intracolonically 
      (i.c.) to Wistar-Harlan male rats. Animals were treated (2 times/day) with H(2)S 
      donor Lawesson's reagent per os. Our results showed that H(2)S treatment 
      significantly decreased the extent of colonic lesions. Furthermore, the 
      expression of members of NETs formation: peptidyl arginine deiminase 4 (PAD4), 
      citrullinated histone H3 (citH3), myeloperoxidase (MPO) and inflammatory 
      regulators, such as nuclear transcription factor-kappa B (NF-kappaB) and 
      high-mobility group box 1 (HMGB1) were reduced in H(2)S treated group compared to 
      TNBS. Additionally, H(2)S donor administration elevated the expression of 
      ubiquitin C-terminal hydroxylase L1 (UCHL-1), a potential anti-inflammatory 
      mediator. Taken together, our results showed that H(2)S may exert 
      anti-inflammatory effect through the inhibition of NETs formation, which suggests 
      a new therapeutic approach against IBD.
FAU - Torok, Szilvia
AU  - Torok S
AD  - Department of Physiology, Anatomy and Neuroscience, Faculty of Science and 
      Informatics, University of Szeged, H-6726 Szeged, Hungary.
FAU - Almasi, Nikoletta
AU  - Almasi N
AD  - Department of Physiology, Anatomy and Neuroscience, Faculty of Science and 
      Informatics, University of Szeged, H-6726 Szeged, Hungary.
FAU - Valkusz, Zsuzsanna
AU  - Valkusz Z
AD  - Albert Szent-Gyorgyi Clinical Center, Department of Medicine, Medical Faculty, 
      University of Szeged, H-6720 Szeged, Hungary.
FAU - Posa, Aniko
AU  - Posa A
AD  - Department of Physiology, Anatomy and Neuroscience, Faculty of Science and 
      Informatics, University of Szeged, H-6726 Szeged, Hungary.
AD  - Interdisciplinary Excellence Center, Department of Physiology, Anatomy and 
      Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 
      Szeged, Hungary.
FAU - Varga, Csaba
AU  - Varga C
AD  - Department of Physiology, Anatomy and Neuroscience, Faculty of Science and 
      Informatics, University of Szeged, H-6726 Szeged, Hungary.
FAU - Kupai, Krisztina
AU  - Kupai K
AD  - Department of Physiology, Anatomy and Neuroscience, Faculty of Science and 
      Informatics, University of Szeged, H-6726 Szeged, Hungary.
AD  - Albert Szent-Gyorgyi Clinical Center, Department of Medicine, Medical Faculty, 
      University of Szeged, H-6720 Szeged, Hungary.
LA  - eng
PT  - Journal Article
DEP - 20211125
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Gasotransmitters)
RN  - 0 (Inflammation Mediators)
RN  - 0 (NF-kappa B)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Colitis/chemically induced/*drug therapy/metabolism/pathology
MH  - Extracellular Traps/*drug effects
MH  - Gasotransmitters/pharmacology
MH  - Hydrogen Sulfide/*pharmacology
MH  - Inflammation Mediators/metabolism
MH  - Male
MH  - NF-kappa B/genetics/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction
MH  - Trinitrobenzenesulfonic Acid/*toxicity
PMC - PMC8657984
OTO - NOTNLM
OT  - colitis
OT  - hydrogen sulfide
OT  - inflammation
OT  - inflammatory bowel disease
OT  - neutrophil extracellular traps
COIS- The authors declare no conflict of interest.
EDAT- 2021/12/11 06:00
MHDA- 2021/12/21 06:00
PMCR- 2021/11/25
CRDT- 2021/12/10 01:03
PHST- 2021/10/08 00:00 [received]
PHST- 2021/11/18 00:00 [revised]
PHST- 2021/11/22 00:00 [accepted]
PHST- 2021/12/10 01:03 [entrez]
PHST- 2021/12/11 06:00 [pubmed]
PHST- 2021/12/21 06:00 [medline]
PHST- 2021/11/25 00:00 [pmc-release]
AID - ijms222312729 [pii]
AID - ijms-22-12729 [pii]
AID - 10.3390/ijms222312729 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Nov 25;22(23):12729. doi: 10.3390/ijms222312729.