PMID- 34885059 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20211214 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 13 IP - 23 DP - 2021 Nov 26 TI - Assessment of Ovarian Tumor Growth in Wild-Type and Lumican-Deficient Mice: Insights Using Infrared Spectral Imaging, Histopathology, and Immunohistochemistry. LID - 10.3390/cancers13235950 [doi] LID - 5950 AB - Ovarian cancer remains one of the most fatal cancers due to a lack of robust screening methods of detection at early stages. Extracellular matrix (ECM) mediates interactions between cancer cells and their microenvironment via specific molecules. Lumican, a small leucine-rich proteoglycan (SLRP), maintains ECM integrity and inhibits both melanoma primary tumor development, as well as metastatic spread. The aim of this study was to analyze the effect of lumican on tumor growth of murine ovarian epithelial cancer. C57BL/6 wild type mice (n = 12) and lumican-deficient mice (n = 10) were subcutaneously injected with murine ovarian epithelial carcinoma ID8 cells, and then sacrificed after 18 days. Analysis of tumor volumes demonstrated an inhibitory effect of endogenous lumican on ovarian tumor growth. The ovarian primary tumors were subjected to histological and immunohistochemical staining using anti-lumican, anti-alphav integrin, anti-CD31 and anti-cyclin D1 antibodies, and then further examined by label-free infrared spectral imaging (IRSI), second harmonic generation (SHG) and Picrosirius red staining. The IR tissue images allowed for the identification of different ECM tissue regions of the skin and the ovarian tumor. Moreover, IRSI showed a good correlation with alphav integrin immunostaining and collagen organization within the tumor. Our results demonstrate that lumican inhibits ovarian cancer growth mainly by altering collagen fibrilogenesis. FAU - Nizet, Pierre AU - Nizet P AUID- ORCID: 0000-0002-6224-8146 AD - Laboratoire de Biochimie Medicale et Biologie Moleculaire, UFR Medecine, Universite de Reims Champagne-Ardenne, 51097 Reims, France. AD - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, 51097 Reims, France. FAU - Untereiner, Valerie AU - Untereiner V AUID- ORCID: 0000-0002-3360-0171 AD - Universite de Reims Champagne-Ardenne, PICT, 51097 Reims, France. FAU - Sockalingum, Ganesh D AU - Sockalingum GD AUID- ORCID: 0000-0003-3160-4678 AD - Universite de Reims Champagne-Ardenne, BioSpecT-EA7506, UFR Pharmacie, 51097 Reims, France. FAU - Proult, Isabelle AU - Proult I AUID- ORCID: 0000-0003-0236-7132 AD - Laboratoire de Biochimie Medicale et Biologie Moleculaire, UFR Medecine, Universite de Reims Champagne-Ardenne, 51097 Reims, France. AD - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, 51097 Reims, France. FAU - Terryn, Christine AU - Terryn C AUID- ORCID: 0000-0003-1410-4524 AD - Universite de Reims Champagne-Ardenne, PICT, 51097 Reims, France. FAU - Jeanne, Albin AU - Jeanne A AUID- ORCID: 0000-0003-2930-4158 AD - Laboratoire de Biochimie Medicale et Biologie Moleculaire, UFR Medecine, Universite de Reims Champagne-Ardenne, 51097 Reims, France. AD - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, 51097 Reims, France. AD - Apmonia Therapeutics SAS, CREA, 2 esplanade Roland Garros, 51686 Reims, France. FAU - Nannan, Lise AU - Nannan L AUID- ORCID: 0000-0001-5680-8074 AD - Laboratoire de Biochimie Medicale et Biologie Moleculaire, UFR Medecine, Universite de Reims Champagne-Ardenne, 51097 Reims, France. AD - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, 51097 Reims, France. FAU - Boulagnon-Rombi, Camille AU - Boulagnon-Rombi C AUID- ORCID: 0000-0001-5610-1722 AD - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, 51097 Reims, France. AD - CHU Reims, Service de Pathologie, 51092 Reims, France. FAU - Sellier, Christele AU - Sellier C AUID- ORCID: 0000-0001-5474-7394 AD - Laboratoire de Biochimie Medicale et Biologie Moleculaire, UFR Medecine, Universite de Reims Champagne-Ardenne, 51097 Reims, France. AD - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, 51097 Reims, France. FAU - Rivet, Romain AU - Rivet R AUID- ORCID: 0000-0002-7290-6069 AD - Laboratoire de Biochimie Medicale et Biologie Moleculaire, UFR Medecine, Universite de Reims Champagne-Ardenne, 51097 Reims, France. AD - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, 51097 Reims, France. FAU - Ramont, Laurent AU - Ramont L AUID- ORCID: 0000-0002-3046-8021 AD - Laboratoire de Biochimie Medicale et Biologie Moleculaire, UFR Medecine, Universite de Reims Champagne-Ardenne, 51097 Reims, France. AD - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, 51097 Reims, France. AD - CHU Reims, Service de Biochimie-Pharmacologie-Toxicologie, 51092 Reims, France. FAU - Brezillon, Stephane AU - Brezillon S AUID- ORCID: 0000-0001-7954-8340 AD - Laboratoire de Biochimie Medicale et Biologie Moleculaire, UFR Medecine, Universite de Reims Champagne-Ardenne, 51097 Reims, France. AD - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, 51097 Reims, France. LA - eng PT - Journal Article DEP - 20211126 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC8656468 OTO - NOTNLM OT - K-means clustering OT - collagen OT - glycosaminoglycan OT - immunohistochemistry OT - infrared imaging OT - lumican OT - ovarian cancer OT - second harmonic generation COIS- The authors declare no conflict of interest. EDAT- 2021/12/11 06:00 MHDA- 2021/12/11 06:01 PMCR- 2021/11/26 CRDT- 2021/12/10 01:05 PHST- 2021/10/29 00:00 [received] PHST- 2021/11/22 00:00 [revised] PHST- 2021/11/23 00:00 [accepted] PHST- 2021/12/10 01:05 [entrez] PHST- 2021/12/11 06:00 [pubmed] PHST- 2021/12/11 06:01 [medline] PHST- 2021/11/26 00:00 [pmc-release] AID - cancers13235950 [pii] AID - cancers-13-05950 [pii] AID - 10.3390/cancers13235950 [doi] PST - epublish SO - Cancers (Basel). 2021 Nov 26;13(23):5950. doi: 10.3390/cancers13235950.