PMID- 34889727
OWN - NLM
STAT- MEDLINE
DCOM- 20221229
LR  - 20221229
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Print)
IS  - 0022-2615 (Linking)
VI  - 70
IP  - 12
DP  - 2021 Dec
TI  - Additional C-type lectin receptors mediate interactions with Pneumocystis 
      organisms and major surface glycoprotein.
LID - 10.1099/jmm.0.001470 [doi]
LID - 001470
AB  - Introduction. Pathogen-associated molecular patterns' (PAMPs) are microbial 
      signatures that are recognized by host myeloid C-type lectin receptors (CLRs). 
      These CLRs interact with micro-organisms via their carbohydrate recognition 
      domains (CRDs) and engage signalling pathways within the cell resulting in 
      pro-inflammatory and microbicidal responses.Gap statement. In this article, we 
      extend our laboratory study of additional CLRs that recognize fungal ligands 
      against Pneumocystis murina and Pneumocystis carinii and their purified major 
      surface glycoproteins (Msgs).Aim. To study the potential of newly synthesized 
      hFc-CLR fusions on binding to Pneumocystis and its Msg.Methods. A library of new 
      synthesized hFc-CLR fusions was screened against Pneumocystis murina and 
      Pneumocystis carinii organisms and their purified major surface glycoproteins 
      (Msgs) found on the respective fungi via modified ELISA. Immunofluorescence assay 
      (IFA) was implemented and quantified to verify results. mRNA expression analysis 
      by quantitative PCR (q-PCR) was employed to detect respective CLRs found to bind 
      fungal organisms in the ELISA and determine their expression levels in the mouse 
      immunosuppressed Pneumocystis pneumonia (PCP) model.Results. We detected a number 
      of the CLR hFc-fusions displayed significant binding with P. murina and P. 
      carinii organisms, and similarly to their respective Msgs. Significant organism 
      and Msg binding was observed for CLR members C-type lectin domain family 12 
      member A (CLEC12A), Langerin, macrophage galactose-type lectin-1 (MGL-1), and 
      specific intracellular adhesion molecule-3 grabbing non-integrin 
      homologue-related 3 (SIGNR3). Immunofluorescence assay (IFA) with the respective 
      CLR hFc-fusions against whole P. murina life forms corroborated these findings. 
      Lastly, we surveyed the mRNA expression profiles of the respective CLRs tested 
      above in the mouse immunosuppressed Pneumocystis pneumonia (PCP) model and 
      determined that macrophage galactose type C-type lectin (Mgl-1), implicated in 
      recognizing terminal N-acetylgalactosamine (GalNAc) found in the glycoproteins of 
      microbial pathogens was significantly up-regulated during infection.Conclusion. 
      The data herein add to the growing list of CLRs recognizing Pneumocystis and 
      provide insights for further study of organism/host immune cell interactions.
FAU - Kottom, Theodore J
AU  - Kottom TJ
AD  - Thoracic Diseases Research Unit, Departments of Medicine and Biochemistry, Mayo 
      Clinic College of Medicine, Rochester, MN, USA.
FAU - Carmona, Eva M
AU  - Carmona EM
AD  - Thoracic Diseases Research Unit, Departments of Medicine and Biochemistry, Mayo 
      Clinic College of Medicine, Rochester, MN, USA.
FAU - Schaefbauer, Kyle
AU  - Schaefbauer K
AD  - Thoracic Diseases Research Unit, Departments of Medicine and Biochemistry, Mayo 
      Clinic College of Medicine, Rochester, MN, USA.
FAU - Limper, Andrew H
AU  - Limper AH
AD  - Thoracic Diseases Research Unit, Departments of Medicine and Biochemistry, Mayo 
      Clinic College of Medicine, Rochester, MN, USA.
LA  - eng
GR  - R01 HL062150/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Fungal Proteins)
RN  - X2RN3Q8DNE (Galactose)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - Pneumocystis murina
SB  - IM
MH  - Animals
MH  - Mice
MH  - Fungal Proteins
MH  - Galactose
MH  - Host-Pathogen Interactions
MH  - *Lectins, C-Type/genetics
MH  - *Membrane Glycoproteins
MH  - *Pneumocystis/genetics
MH  - Pneumocystis carinii/genetics
MH  - *Pneumonia, Pneumocystis/immunology
MH  - RNA, Messenger
PMC - PMC8744274
OTO - NOTNLM
OT  - C-type lectin receptors (CLRs)
OT  - carbohydrate recognition domains (CRDs)
OT  - major surface glycoprotein (Msg)
OT  - pneumocystis
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2021/12/11 06:00
MHDA- 2021/12/30 06:00
PMCR- 2021/12/10
CRDT- 2021/12/10 12:15
PHST- 2021/12/10 12:15 [entrez]
PHST- 2021/12/11 06:00 [pubmed]
PHST- 2021/12/30 06:00 [medline]
PHST- 2021/12/10 00:00 [pmc-release]
AID - 001470 [pii]
AID - 10.1099/jmm.0.001470 [doi]
PST - ppublish
SO  - J Med Microbiol. 2021 Dec;70(12):001470. doi: 10.1099/jmm.0.001470.