PMID- 34890540
OWN - NLM
STAT- MEDLINE
DCOM- 20220214
LR  - 20220531
IS  - 1873-6254 (Electronic)
IS  - 0001-706X (Linking)
VI  - 226
DP  - 2022 Feb
TI  - Phytochemical, anti-Acanthamoeba, and anti-adhesion properties of Garcinia 
      mangostana flower as preventive contact lens solution.
PG  - 106266
LID - S0001-706X(21)00444-7 [pii]
LID - 10.1016/j.actatropica.2021.106266 [doi]
AB  - Acanthamoeba keratitis infection extends due to the growing number of contact 
      lens users. Indigenous plants including Garcinia mangostana play a vital role in 
      human health and well being. Many species of this plant have been reported with 
      myriads of potent medicinal properties. However, the aims of this study were, for 
      the first time, to isolate compounds from the flower of G. mangostana and to test 
      their anti-Acanthamoeba and anti-adhesion activity against Acanthamoeba 
      triangularis. Powdered flowers of G. mangostana were extracted and 
      chromatographed on a silica gel column. The structures of the compounds were 
      established with the aid of (1)H NMR. More so, the anti-Acanthamoeba and 
      anti-adhesion properties were tested on a 96-well polystyrene microtiter plate 
      and soft contact lenses. Scanning electron microscope (SEM) was used to determine 
      the features of A. triangularis on contact lenses. Eight pure compounds were 
      obtained, namely 9-hydroxycalabaxanthone, tovophillin A, garcinone E, garcinone 
      B, alpha-mangostin, gartinin, 8-deoxygartinin and gamma-mangostin. The extract and pure 
      compounds exhibited anti-Acanthamoeba activity with MIC values in the range of 
      0.25-1 mg/mL. In addition, the extract and alpha-mangostin displayed significant 
      activity against the adhesion of A. triangularis trophozoites both in polystyrene 
      plate and in contact lenses at 0.5 x MIC (0.25 mg/mL). Furthermore, alpha-mangostin 
      has the potential to remove A. triangularis adhesion in contact lenses similar to 
      a commercial multipurpose solution (MPS). SEM study confirmed that crude extract 
      and alpha-mangostin are effective as solutions for contact lenses, which removed A. 
      triangularis trophozoites within 24 h. Alpha-mangostin was non-toxic to Vero 
      cells at a concentration below 39 muM in 24 h. Crude extract of G. mangostana 
      flower and its alpha-mangostin serve as candidate compounds in the treatment of 
      Acanthamoeba infection or as lens care solution, since they can be used as a 
      source of natural products against Acanthamoeba and virulence factor associated 
      with the adhesion of A. triangularis.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Sangkanu, Suthinee
AU  - Sangkanu S
AD  - School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), 
      World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center 
      for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si 
      Thammarat, Thailand.
FAU - Mitsuwan, Watcharapong
AU  - Mitsuwan W
AD  - Akkhraratchumari Veterinary College, Walailak University, Nakhon Si Thammarat, 
      Thailand.
FAU - Mahboob, Tooba
AU  - Mahboob T
AD  - School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), 
      World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center 
      for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si 
      Thammarat, Thailand.
FAU - Mahabusarakam, Wilawan
AU  - Mahabusarakam W
AD  - Division of Physical Science, Faculty of Science, Graduate School, Prince of 
      Songkla University, Songkhla 90110, Thailand. Electronic address: 
      wilawan.m@psu.ac.th.
FAU - Chewchanwuttiwong, Sopa
AU  - Chewchanwuttiwong S
AD  - Faculty of Science and Industrial Technology, Prince of Songkla University, Surat 
      Thani Campus, Makhamtae, Surat Thani, Muang, Thailand.
FAU - Siphakdi, Panisara
AU  - Siphakdi P
AD  - Faculty of Science and Industrial Technology, Prince of Songkla University, Surat 
      Thani Campus, Makhamtae, Surat Thani, Muang, Thailand.
FAU - Jimoh, Tajudeen O
AU  - Jimoh TO
AD  - Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical 
      Sciences, Chulalongkorn University, Bangkok, Thailand; Department of 
      Biochemistry, Faculty of Health Sciences, Islamic University in Uganda, P. O. Box 
      7689, Kampala, Uganda.
FAU - Wilairatana, Polrat
AU  - Wilairatana P
AD  - Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol 
      University, Bangkok, Thailand.
FAU - Dolma, Karma G
AU  - Dolma KG
AD  - Department of Microbiology, Sikkim Manipal Institute of Medical Sciences, Sikkim, 
      India.
FAU - Pereira, Maria de Lourdes
AU  - Pereira ML
AD  - Department of Medical Sciences, CICECO-Aveiro Institute of Materials, University 
      of Aveiro, Aveiro, Portugal.
FAU - Rahmatullah, Mohammed
AU  - Rahmatullah M
AD  - Department of Biotechnology & Genetic Engineering, University of Development 
      Alternative, Lalmatia, Dhaka, Bangladesh.
FAU - Wiart, Christophe
AU  - Wiart C
AD  - Institute for Tropical Biology & Conservation, University Malaysia Sabah, 
      Malaysia.
FAU - Norouzi, Roghayeh
AU  - Norouzi R
AD  - Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, 
      Tabriz, Iran.
FAU - Siyadatpanah, Abolghasem
AU  - Siyadatpanah A
AD  - Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, 
      Birjand, Iran.
FAU - Mutombo, Polydor Ngoy
AU  - Mutombo PN
AD  - National Centre for Naturopathic Medicine, Faculty of Health Sciences, Southern 
      Cross University, Lismore, NSW, Australia.
FAU - Nissapatorn, Veeranoot
AU  - Nissapatorn V
AD  - School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), 
      World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center 
      for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si 
      Thammarat, Thailand.. Electronic address: nissapat@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20211208
PL  - Netherlands
TA  - Acta Trop
JT  - Acta tropica
JID - 0370374
RN  - 0 (Contact Lens Solutions)
RN  - 0 (Phytochemicals)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - *Acanthamoeba/drug effects
MH  - Animals
MH  - Chlorocebus aethiops
MH  - *Contact Lens Solutions
MH  - Flowers/chemistry
MH  - *Garcinia mangostana/chemistry
MH  - Humans
MH  - Phytochemicals/pharmacology
MH  - Plant Extracts/*pharmacology
MH  - Vero Cells
OTO - NOTNLM
OT  - Acanthamoeba triangularis
OT  - Anti-Acanthamoeba activity
OT  - Anti-adhesion activity
OT  - Contact lens
OT  - Garcinia mangostana flower
OT  - alpha-Mangostin
EDAT- 2021/12/11 06:00
MHDA- 2022/02/15 06:00
CRDT- 2021/12/10 20:12
PHST- 2021/04/05 00:00 [received]
PHST- 2021/12/01 00:00 [revised]
PHST- 2021/12/05 00:00 [accepted]
PHST- 2021/12/11 06:00 [pubmed]
PHST- 2022/02/15 06:00 [medline]
PHST- 2021/12/10 20:12 [entrez]
AID - S0001-706X(21)00444-7 [pii]
AID - 10.1016/j.actatropica.2021.106266 [doi]
PST - ppublish
SO  - Acta Trop. 2022 Feb;226:106266. doi: 10.1016/j.actatropica.2021.106266. Epub 2021 
      Dec 8.