PMID- 34891899
OWN - NLM
STAT- MEDLINE
DCOM- 20211230
LR  - 20211230
IS  - 2694-0604 (Electronic)
IS  - 2375-7477 (Linking)
VI  - 2021
DP  - 2021 Nov
TI  - Antral Variation of Murine Gastric Pacemaker Cells Informed by Confocal Imaging 
      and Machine Learning Methods.
PG  - 3105-3108
LID - 10.1109/EMBC46164.2021.9630341 [doi]
AB  - The Interstitial Cells of Cajal (ICC) are specialized gastrointestinal (GI) 
      pacemaker cells that generate and actively propagate electrophysiological events 
      called slow waves. Slow waves regulate the GI motility necessary for digestion. 
      Several functional GI motility disorders have been associated with depletion in 
      the ICC. In this study, a validated Fast Random Forest (FRF) classification 
      method using Trainable WEKA Segmentation for segmenting the networks of ICC was 
      applied to confocal microscopy images of a whole mount tissue from the distal 
      antrum of a mouse stomach (583 x 3,376 x 133 mum(3), parcellated into 24 equal 
      image stacks). The FRF model performance was compared to 6 manually segmented 
      subflelds and produced an area under the receiver-operating characteristic 
      (AUROC) of 0.95. Structural variations of ICC network in the longitudinal muscle 
      (ICC-LM) and myenteric plexus (ICC-MP) were quantified. The average volume of 
      ICC-MP was significantly higher than ICC-LM at any point throughout the antral 
      tissue sampled. There was a pronounced decline of up to 80% in ICC-LM (from 3,705 
      mum(3) to 716 mum(3)) over a distance of 279.3 mum, that eventually diminished 
      towards the distal antrum. However, an inverse relationship was observed in 
      ICC-MP with an overall increase of up to 157% (from 59,100 mum(3) to 151,830 
      mum(3)) over a distance of approximately 2 mm that proceeds towards the distal 
      antrum.
FAU - Mah, Sue Ann
AU  - Mah SA
FAU - Avci, Recep
AU  - Avci R
FAU - Du, Peng
AU  - Du P
FAU - Vanderwinden, Jean-Marie
AU  - Vanderwinden JM
FAU - Cheng, Leo K
AU  - Cheng LK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Annu Int Conf IEEE Eng Med Biol Soc
JT  - Annual International Conference of the IEEE Engineering in Medicine and Biology 
      Society. IEEE Engineering in Medicine and Biology Society. Annual International 
      Conference
JID - 101763872
SB  - IM
MH  - Animals
MH  - Gastrointestinal Motility
MH  - *Interstitial Cells of Cajal
MH  - Machine Learning
MH  - Mice
MH  - Microscopy, Confocal
MH  - Myenteric Plexus
EDAT- 2021/12/12 06:00
MHDA- 2021/12/31 06:00
CRDT- 2021/12/11 01:03
PHST- 2021/12/11 01:03 [entrez]
PHST- 2021/12/12 06:00 [pubmed]
PHST- 2021/12/31 06:00 [medline]
AID - 10.1109/EMBC46164.2021.9630341 [doi]
PST - ppublish
SO  - Annu Int Conf IEEE Eng Med Biol Soc. 2021 Nov;2021:3105-3108. doi: 
      10.1109/EMBC46164.2021.9630341.