PMID- 34893275
OWN - NLM
STAT- MEDLINE
DCOM- 20220316
LR  - 20220316
IS  - 1879-1344 (Electronic)
IS  - 0144-8617 (Linking)
VI  - 277
DP  - 2022 Feb 1
TI  - Relationship between phosphorylamine-modification and molecular weight on 
      transfection efficiency of chitosan.
PG  - 118870
LID - S0144-8617(21)01257-1 [pii]
LID - 10.1016/j.carbpol.2021.118870 [doi]
AB  - The aim of this study is both to design the chitosan (Chi) nanoparticles with 
      different M(w) containing the phosphoester bonds and increase their amino 
      function for the transfection. The phosphorylamine-modification of Chi and 
      depolymerized Chi (DChi) was realized using o-phosphorylethanolamine (o-PEA) and 
      characterized, for the first time. The nanoparticles (nMChi and nMDChi) were 
      prepared by ionic gelation and their particle size, polydispersity index (PDI), 
      zeta potential, stability, gene binding capacity and cytotoxicity were examined. 
      The effects of the M(w) of Chi on the cytotoxicity, gene binding capacity, and in 
      vitro transfection efficiency of the nanoparticles on Human Embryonic Kidney 293 
      (HEK293) cells were also examined. Green Fluorescent Protein Circular Plasmid DNA 
      (pEGFN1) loaded nanoparticles (gnMChi and gnMDChi) were used in the transfection. 
      This study showed that the M(w) of phosphorylamine-modified Chi significantly 
      affected the characteristics, cytotoxicity, gene binding capacity and 
      transfection efficiency of the nanoparticles.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Celik, Sibel
AU  - Celik S
AD  - Department of Chemical Engineering, Faculty of Engineering, Istanbul 
      University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey.
FAU - Gok, Mehmet Koray
AU  - Gok MK
AD  - Department of Chemical Engineering, Faculty of Engineering, Istanbul 
      University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey.
FAU - Demir, Kamber
AU  - Demir K
AD  - Department of Reproduction and Artificial Insemination, Faculty of Veterinary 
      Medicine, Istanbul University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey.
FAU - Pabuccuoglu, Serhat
AU  - Pabuccuoglu S
AD  - Department of Reproduction and Artificial Insemination, Faculty of Veterinary 
      Medicine, Istanbul University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey.
FAU - Ozgumus, Saadet
AU  - Ozgumus S
AD  - Department of Chemical Engineering, Faculty of Engineering, Istanbul 
      University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey. Electronic address: 
      saadetk@iuc.edu.tr.
LA  - eng
PT  - Journal Article
DEP - 20211106
PL  - England
TA  - Carbohydr Polym
JT  - Carbohydrate polymers
JID - 8307156
RN  - 0 (Amines)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Amines/*chemistry
MH  - Carbohydrate Conformation
MH  - Cell Survival/drug effects
MH  - Chitosan/chemical synthesis/*chemistry/pharmacology
MH  - HEK293 Cells
MH  - Humans
MH  - Molecular Weight
MH  - Nanoparticles/chemistry
MH  - Particle Size
OTO - NOTNLM
OT  - Chitosan
OT  - Gene carrier
OT  - Nanoparticle
OT  - Phosphorylation
OT  - Transfection
EDAT- 2021/12/12 06:00
MHDA- 2022/03/17 06:00
CRDT- 2021/12/11 05:29
PHST- 2021/02/18 00:00 [received]
PHST- 2021/10/09 00:00 [revised]
PHST- 2021/11/05 00:00 [accepted]
PHST- 2021/12/11 05:29 [entrez]
PHST- 2021/12/12 06:00 [pubmed]
PHST- 2022/03/17 06:00 [medline]
AID - S0144-8617(21)01257-1 [pii]
AID - 10.1016/j.carbpol.2021.118870 [doi]
PST - ppublish
SO  - Carbohydr Polym. 2022 Feb 1;277:118870. doi: 10.1016/j.carbpol.2021.118870. Epub 
      2021 Nov 6.