PMID- 34893295
OWN - NLM
STAT- MEDLINE
DCOM- 20220316
LR  - 20220316
IS  - 1879-1344 (Electronic)
IS  - 0144-8617 (Linking)
VI  - 277
DP  - 2022 Feb 1
TI  - beta(2-->6)-Type fructans attenuate proinflammatory responses in a structure dependent 
      fashion via Toll-like receptors.
PG  - 118893
LID - S0144-8617(21)01280-7 [pii]
LID - 10.1016/j.carbpol.2021.118893 [doi]
AB  - Graminan-type fructans (GTFs) have demonstrated immune benefits. However, 
      mechanisms underlying these benefits are unknown. We studied GTFs interaction 
      with Toll-like receptors (TLRs), performed molecular docking and determined their 
      impact on dendritic cells (DCs). Effects of GTFs were compared with those of 
      inulin-type fructans (ITFs). Whereas ITFs only contained beta(2-->1)-linked fructans, 
      GTFs showed higher complexity as it contains additional beta(2-->6)-linkages. GTFs 
      activated NF-kappaB/AP-1 through MyD88 and TRIF pathways. GTFs stimulated TLR3, 7 and 
      9 while ITFs activated TLR2 and TLR4. GTFs strongly inhibited TLR2 and TLR4, 
      while ITFs did not inhibit any TLR. Molecular docking demonstrated interactions 
      of fructans with TLR2, 3, and 4 in a structure dependent fashion. Moreover, ITFs 
      and GTFs attenuated pro-inflammatory cytokine production of stimulated DCs. These 
      findings demonstrate immunomodulatory effects of GTFs via TLRs and attenuation of 
      cytokine production in dendritic cells by GTFs and long-chain ITF.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Fernandez-Lainez, C
AU  - Fernandez-Lainez C
AD  - Immunoendocrinology, Division of Medical Biology, Department of Pathology and 
      Medical Biology, University of Groningen and University Medical Center Groningen, 
      Hanzeplein 1, 9700 RB Groningen, the Netherlands; Laboratorio de Errores Innatos 
      del Metabolismo y Tamiz, Instituto Nacional de Pediatria, Ciudad de Mexico, 
      Mexico; Posgrado en Ciencias Biologicas, Universidad Nacional Autonoma de Mexico 
      UNAM, Ciudad de Mexico, Mexico. Electronic address: c.fernandez.lainez@umcg.nl.
FAU - Akkerman, R
AU  - Akkerman R
AD  - Immunoendocrinology, Division of Medical Biology, Department of Pathology and 
      Medical Biology, University of Groningen and University Medical Center Groningen, 
      Hanzeplein 1, 9700 RB Groningen, the Netherlands.
FAU - Oerlemans, M M P
AU  - Oerlemans MMP
AD  - Immunoendocrinology, Division of Medical Biology, Department of Pathology and 
      Medical Biology, University of Groningen and University Medical Center Groningen, 
      Hanzeplein 1, 9700 RB Groningen, the Netherlands.
FAU - Logtenberg, M J
AU  - Logtenberg MJ
AD  - Laboratory of Food Chemistry, Wageningen University, Wageningen, the Netherlands.
FAU - Schols, H A
AU  - Schols HA
AD  - Laboratory of Food Chemistry, Wageningen University, Wageningen, the Netherlands.
FAU - Silva-Lagos, L A
AU  - Silva-Lagos LA
AD  - Immunoendocrinology, Division of Medical Biology, Department of Pathology and 
      Medical Biology, University of Groningen and University Medical Center Groningen, 
      Hanzeplein 1, 9700 RB Groningen, the Netherlands.
FAU - Lopez-Velazquez, G
AU  - Lopez-Velazquez G
AD  - Laboratorio de Biomoleculas y Salud Infantil, Instituto Nacional de Pediatria, 
      Ciudad de Mexico, Mexico.
FAU - de Vos, P
AU  - de Vos P
AD  - Immunoendocrinology, Division of Medical Biology, Department of Pathology and 
      Medical Biology, University of Groningen and University Medical Center Groningen, 
      Hanzeplein 1, 9700 RB Groningen, the Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20211115
PL  - England
TA  - Carbohydr Polym
JT  - Carbohydrate polymers
JID - 8307156
RN  - 0 (Fructans)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Carbohydrate Conformation
MH  - Cell Line
MH  - Fructans/*immunology
MH  - Humans
MH  - Inflammation/*immunology
MH  - Toll-Like Receptors/*immunology
OTO - NOTNLM
OT  - Branched-chain fructans
OT  - Functional food
OT  - Immunomodulation
OT  - Non-digestible carbohydrates
OT  - Toll-like receptors
EDAT- 2021/12/12 06:00
MHDA- 2022/03/17 06:00
CRDT- 2021/12/11 05:29
PHST- 2021/07/31 00:00 [received]
PHST- 2021/10/25 00:00 [revised]
PHST- 2021/11/11 00:00 [accepted]
PHST- 2021/12/11 05:29 [entrez]
PHST- 2021/12/12 06:00 [pubmed]
PHST- 2022/03/17 06:00 [medline]
AID - S0144-8617(21)01280-7 [pii]
AID - 10.1016/j.carbpol.2021.118893 [doi]
PST - ppublish
SO  - Carbohydr Polym. 2022 Feb 1;277:118893. doi: 10.1016/j.carbpol.2021.118893. Epub 
      2021 Nov 15.