PMID- 34896115
OWN - NLM
STAT- MEDLINE
DCOM- 20220117
LR  - 20220117
IS  - 1090-2430 (Electronic)
IS  - 0014-4886 (Linking)
VI  - 348
DP  - 2022 Feb
TI  - FGF binding protein 3 is required for spinal cord motor neuron development and 
      regeneration in zebrafish.
PG  - 113944
LID - S0014-4886(21)00352-6 [pii]
LID - 10.1016/j.expneurol.2021.113944 [doi]
AB  - Fibroblast growth factor binding protein 3 (Fgfbp3) have been known to be crucial 
      for the process of neural proliferation, differentiation, migration, and 
      adhesion. However, the specific role and the molecular mechanisms of fgfbp3 in 
      regulating the development of motor neurons remain unclear. In this study, we 
      have investigated the function of fgfbp3 in morphogenesis and regeneration of 
      motor neuron in zebrafish. Firstly, we found that fgfbp3 was localized in the 
      motor neurons and loss of fgfbp3 caused the significant decrease of the length 
      and branching number of the motor neuron axons, which could be partially rescued 
      by fgfbp3 mRNA injection. Moreover, the fgfbp3 knockdown (KD) embryos 
      demonstrated similar defects of motor neurons as identified in fgfbp3 knockout 
      (KO) embryos. Furthermore, we revealed that the locomotion and startle response 
      of fgfbp3 KO embryos were significantly restricted, which were partially rescued 
      by the fgfbp3 overexpression. In addition, fgfbp3 KO remarkably compromised 
      axonal regeneration of motor neurons after injury. Lastly, the malformation of 
      motor neurons in fgfbp3 KO embryos was rescued by overexpressing drd1b or 
      neurod6a, respectively, which were screened by transcriptome sequencing. Taken 
      together, our results provide strong cellular and molecular evidence that fgfbp3 
      is crucial for the axonal morphogenesis and regeneration of motor neurons in 
      zebrafish.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Xu, Guangmin
AU  - Xu G
AD  - School of Life Science, Nantong Laboratory of Development and Diseases; Second 
      Affiliated Hospital; Key Laboratory of Neuroregeneration of Jiangsu and Ministry 
      of Education, Co-innovation Center of Neuroregeneration, Nantong University, 
      Nantong, China.
FAU - Huang, Zigang
AU  - Huang Z
AD  - School of Life Science, Nantong Laboratory of Development and Diseases; Second 
      Affiliated Hospital; Key Laboratory of Neuroregeneration of Jiangsu and Ministry 
      of Education, Co-innovation Center of Neuroregeneration, Nantong University, 
      Nantong, China.
FAU - Sheng, Jiajing
AU  - Sheng J
AD  - School of Life Science, Nantong Laboratory of Development and Diseases; Second 
      Affiliated Hospital; Key Laboratory of Neuroregeneration of Jiangsu and Ministry 
      of Education, Co-innovation Center of Neuroregeneration, Nantong University, 
      Nantong, China.
FAU - Gao, Xiang
AU  - Gao X
AD  - School of Life Science, Nantong Laboratory of Development and Diseases; Second 
      Affiliated Hospital; Key Laboratory of Neuroregeneration of Jiangsu and Ministry 
      of Education, Co-innovation Center of Neuroregeneration, Nantong University, 
      Nantong, China.
FAU - Wang, Xin
AU  - Wang X
AD  - School of Life Science, Nantong Laboratory of Development and Diseases; Second 
      Affiliated Hospital; Key Laboratory of Neuroregeneration of Jiangsu and Ministry 
      of Education, Co-innovation Center of Neuroregeneration, Nantong University, 
      Nantong, China.
FAU - Garcia, Jason Q
AU  - Garcia JQ
AD  - Department of Bioengineering and Therapeutic Sciences, University of California, 
      San Francisco, San Francisco, CA 94143, USA.
FAU - Wei, Guanyun
AU  - Wei G
AD  - School of Life Science, Nantong Laboratory of Development and Diseases; Second 
      Affiliated Hospital; Key Laboratory of Neuroregeneration of Jiangsu and Ministry 
      of Education, Co-innovation Center of Neuroregeneration, Nantong University, 
      Nantong, China.
FAU - Liu, Dong
AU  - Liu D
AD  - School of Life Science, Nantong Laboratory of Development and Diseases; Second 
      Affiliated Hospital; Key Laboratory of Neuroregeneration of Jiangsu and Ministry 
      of Education, Co-innovation Center of Neuroregeneration, Nantong University, 
      Nantong, China. Electronic address: tom@ntu.edu.cn.
FAU - Gong, Jie
AU  - Gong J
AD  - School of Life Science, Nantong Laboratory of Development and Diseases; Second 
      Affiliated Hospital; Key Laboratory of Neuroregeneration of Jiangsu and Ministry 
      of Education, Co-innovation Center of Neuroregeneration, Nantong University, 
      Nantong, China. Electronic address: jgong188@ntu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211209
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Carrier Proteins)
RN  - 0 (fibroblast growth factor binding protein 3, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Carrier Proteins/antagonists & inhibitors/*biosynthesis/*genetics
MH  - Gene Knockout Techniques/methods
MH  - Motor Neurons/*metabolism
MH  - Nerve Regeneration/*physiology
MH  - Neurogenesis/*physiology
MH  - Reflex, Startle/physiology
MH  - Spinal Cord/growth & development/*metabolism
MH  - Swimming/physiology
MH  - Zebrafish
OTO - NOTNLM
OT  - Fgfbp3
OT  - Morphogenesis
OT  - Motor neurons
OT  - Regeneration
OT  - Zebrafish
EDAT- 2021/12/14 06:00
MHDA- 2022/01/18 06:00
CRDT- 2021/12/13 12:14
PHST- 2021/09/19 00:00 [received]
PHST- 2021/11/10 00:00 [revised]
PHST- 2021/11/30 00:00 [accepted]
PHST- 2021/12/14 06:00 [pubmed]
PHST- 2022/01/18 06:00 [medline]
PHST- 2021/12/13 12:14 [entrez]
AID - S0014-4886(21)00352-6 [pii]
AID - 10.1016/j.expneurol.2021.113944 [doi]
PST - ppublish
SO  - Exp Neurol. 2022 Feb;348:113944. doi: 10.1016/j.expneurol.2021.113944. Epub 2021 
      Dec 9.