PMID- 34897591 OWN - NLM STAT- MEDLINE DCOM- 20220404 LR - 20231031 IS - 1993-0402 (Electronic) IS - 1672-0415 (Linking) VI - 28 IP - 4 DP - 2022 Apr TI - Pharmacodynamic Mechanism of Kuanxiong Aerosol for Vasodilation and Improvement of Myocardial Ischemia. PG - 319-329 LID - 10.1007/s11655-021-2882-z [doi] AB - OBJECTIVE: To explore the effect of Kuanxiong Aerosol (KXA) on isoproterenol (ISO)-induced myocardial injury in rat models. METHODS: Totally 24 rats were radomly divided into control, ISO, KXA low-dose and high-dose groups according to the randomized block design method, and were administered by intragastric administration for 10 consecutive days, and on the 9th and 10th days, rats were injected with ISO for 2 consecutive days to construct an acute myocardial ischemia model to evaluate the improvement of myocardial ischemia by KXA. In addition, the diastolic effect of KXA on rat thoracic aorta and its regulation of ion channels were tested by in vitro vascular tension test. The influence of KXA on the expression of calcium-CaM-dependent protein kinase II (CaMK II)/extracellular regulated protein kinases (ERK) signaling pathway has also been tested. RESULTS: KXA significantly reduced the ISO-induced increase in ST-segment, interventricular septal thickness, cardiac mass index and cardiac tissue pathological changes in rats. Moreover, the relaxation of isolated thoracic arterial rings that had been precontracted using norepinephrine (NE) or potassium chloride (KCl) was increased after KXA treatment in an endothelium-independent manner, and was attenuated by preincubation with verapamil, but not with tetraethylammonium chloride, 4-aminopyridine, glibenclamide, or barium chloride. KXA pretreatment attenuated vasoconstriction induced by CaCl(2) in Ca(2+)-free solutions containing K(+) or NE. In addition, KXA pretreatment inhibited accumulation of Ca(2+) in A7r5 cells mediated by KCl and NE and significantly decreased p-CaMK II and p-ERK levels. CONCLUSION: KXA may inhibit influx and release of calcium and activate the CaMK II/ERK signaling pathway to produce vasodilatory effects, thereby improving myocardial injury. CI - (c) 2021. The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Lu, Yan AU - Lu Y AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. FAU - Yang, Mei-Ling AU - Yang ML AD - Department of Acupuncture and Moxibustion, Third People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. FAU - Shen, A-Ling AU - Shen AL AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. FAU - Lin, Shan AU - Lin S AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. FAU - Peng, Mei-Zhong AU - Peng MZ AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. FAU - Wang, Tian-Yi AU - Wang TY AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. FAU - Lu, Zhu-Qing AU - Lu ZQ AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. FAU - Wang, Yi-Lian AU - Wang YL AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. FAU - Peng, Jun AU - Peng J AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. FAU - Chu, Jian-Feng AU - Chu JF AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. jianfengchu@126.com. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. jianfengchu@126.com. AD - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. jianfengchu@126.com. LA - eng PT - Journal Article DEP - 20211212 PL - China TA - Chin J Integr Med JT - Chinese journal of integrative medicine JID - 101181180 RN - 0 (Aerosols) RN - SY7Q814VUP (Calcium) SB - IM MH - Aerosols MH - Animals MH - Aorta, Thoracic MH - Calcium/metabolism MH - Endothelium, Vascular/metabolism MH - *Myocardial Ischemia/drug therapy/metabolism MH - Rats MH - *Vasodilation OTO - NOTNLM OT - Ca2+ OT - CaMK II/ERK pathway OT - Kuanxiong Aerosol OT - myocardial ischemia OT - vasodilation EDAT- 2021/12/14 06:00 MHDA- 2022/04/05 06:00 CRDT- 2021/12/13 13:32 PHST- 2021/09/05 00:00 [accepted] PHST- 2021/12/14 06:00 [pubmed] PHST- 2022/04/05 06:00 [medline] PHST- 2021/12/13 13:32 [entrez] AID - 10.1007/s11655-021-2882-z [pii] AID - 10.1007/s11655-021-2882-z [doi] PST - ppublish SO - Chin J Integr Med. 2022 Apr;28(4):319-329. doi: 10.1007/s11655-021-2882-z. Epub 2021 Dec 12.