PMID- 34900959
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240404
IS  - 2296-4185 (Print)
IS  - 2296-4185 (Electronic)
IS  - 2296-4185 (Linking)
VI  - 9
DP  - 2021
TI  - Crosstalk Between the Immune System and Plant-Derived Nanovesicles: A Study of 
      Allergen Transporting.
PG  - 760730
LID - 10.3389/fbioe.2021.760730 [doi]
LID - 760730
AB  - Background: Nanometer-sized membrane-surrounded vesicles from different parts of 
      plants including fruits are gaining increasing attention due to their 
      anti-inflammatory and anticancer effects demonstrated by in vitro and in vivo 
      studies, and as nanovectors for molecular delivery of exogenous substances. These 
      nanomaterials are very complex and contain a diverse arsenal of bioactive 
      molecules, such as nucleic acids, proteins, and lipids. Our knowledge about the 
      transport of allergens in vesicles isolated from plant food is limited today. 
      Methods: Here, to investigate the allergenicity of strawberry-derived 
      microvesicles (MVs), nanovesicles (NVs), and subpopulations of NV, we have set up 
      a multidisciplinary approach. The strategy combines proteomics-based protein 
      identification, immunological investigations, bioinformatics, and data mining to 
      gain biological insights useful to evaluate the presence of potential allergens 
      and the immunoglobulin E (IgE) inhibitory activity of vesicle preparations. 
      Results: Immunological test showed that several proteins of strawberry-derived 
      vesicles compete for IgE binding with allergens spotted on the FABER biochip. 
      This includes the known strawberry allergens Fra a 1, Fra a 3, and Fra a 4, and 
      also other IgE-binding proteins not yet described as allergens in this food, such 
      as gibberellin-regulated proteins, 2S albumin, pectate lyase, and trypsin 
      inhibitors. Proteomics identified homologous sequences of the three strawberry 
      allergens and their isoforms in total protein extract (TPE) but only Fra a 1 and 
      Fra a 4 in the vesicle samples. Label-free quantitative proteomic analysis 
      revealed no significant enrichment of these proteins in strawberry vesicles with 
      respect to TPE. Conclusion: Immunological tests and bioinformatics analysis of 
      proteomics data sets revealed that MVs and NVs isolated from strawberries can 
      carry functional allergens their isoforms as well as proteins potentially 
      allergenic based on their structural features. This should be considered when 
      these new nanomaterials are used for human nutraceutical or biomedical 
      applications.
CI  - Copyright (c) 2021 Stanly, Kim, Antonucci, Fiume, Guescini, Kim, Ciardiello, 
      Giangrieco, Mari and Pocsfalvi.
FAU - Stanly, Christopher
AU  - Stanly C
AD  - Institute of Biosciences and Bioresources, National Research Council of Italy, 
      Naples, Italy.
FAU - Kim, Hyoseon
AU  - Kim H
AD  - Department of Applied Chemistry, Institute of Natural Science, Kyung Hee 
      University, Yongin, South Korea.
AD  - Department of Biomedical Science and Technology, Kyung Hee Medical Science 
      Research Institute, Kyung Hee University, Seoul, South Korea.
FAU - Antonucci, Giuseppe
AU  - Antonucci G
AD  - Institute of Biosciences and Bioresources, National Research Council of Italy, 
      Naples, Italy.
FAU - Fiume, Immacolata
AU  - Fiume I
AD  - Institute of Biosciences and Bioresources, National Research Council of Italy, 
      Naples, Italy.
FAU - Guescini, Michele
AU  - Guescini M
AD  - Department of Biomolecular Sciences, University of Urbino Carlo Bo, Urbino, 
      Italy.
FAU - Kim, Kwang Pyo
AU  - Kim KP
AD  - Department of Applied Chemistry, Institute of Natural Science, Kyung Hee 
      University, Yongin, South Korea.
AD  - Department of Biomedical Science and Technology, Kyung Hee Medical Science 
      Research Institute, Kyung Hee University, Seoul, South Korea.
FAU - Ciardiello, Maria Antonietta
AU  - Ciardiello MA
AD  - Institute of Biosciences and Bioresources, National Research Council of Italy, 
      Naples, Italy.
FAU - Giangrieco, Ivana
AU  - Giangrieco I
AD  - Institute of Biosciences and Bioresources, National Research Council of Italy, 
      Naples, Italy.
AD  - Allergy Data Laboratories (ADL), Latina, Italy.
FAU - Mari, Adriano
AU  - Mari A
AD  - Allergy Data Laboratories (ADL), Latina, Italy.
AD  - Associated Centers for Molecular Allergology (CAAM), Rome, Italy.
FAU - Pocsfalvi, Gabriella
AU  - Pocsfalvi G
AD  - Institute of Biosciences and Bioresources, National Research Council of Italy, 
      Naples, Italy.
LA  - eng
PT  - Journal Article
DEP - 20211126
PL  - Switzerland
TA  - Front Bioeng Biotechnol
JT  - Frontiers in bioengineering and biotechnology
JID - 101632513
PMC - PMC8662998
OTO - NOTNLM
OT  - allergens
OT  - nanovesicles
OT  - proteomics
OT  - sequence similarity
OT  - strawberry
COIS- AM as, an ADL stakeholder, develops, manufactures, and distributes the FABER test 
      as a diagnostic service. IG as an employee of ADL contributed to the development, 
      manufacture, and performance of the FABER test. MAC receives funding from ADL.
EDAT- 2021/12/14 06:00
MHDA- 2021/12/14 06:01
PMCR- 2021/01/01
CRDT- 2021/12/13 18:08
PHST- 2021/08/18 00:00 [received]
PHST- 2021/10/05 00:00 [accepted]
PHST- 2021/12/13 18:08 [entrez]
PHST- 2021/12/14 06:00 [pubmed]
PHST- 2021/12/14 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 760730 [pii]
AID - 10.3389/fbioe.2021.760730 [doi]
PST - epublish
SO  - Front Bioeng Biotechnol. 2021 Nov 26;9:760730. doi: 10.3389/fbioe.2021.760730. 
      eCollection 2021.