PMID- 34906590
OWN - NLM
STAT- MEDLINE
DCOM- 20220509
LR  - 20220531
IS  - 1532-8392 (Electronic)
IS  - 0046-8177 (Linking)
VI  - 120
DP  - 2022 Feb
TI  - Pleomorphic adenoma: detection of PLAG1 rearrangement-positive tumor components 
      using whole-slide fluorescence in situ hybridization.
PG  - 26-34
LID - S0046-8177(21)00198-2 [pii]
LID - 10.1016/j.humpath.2021.11.010 [doi]
AB  - Pleomorphic adenoma (PA) consists of heterogeneous histological architecture 
      mixed with epithelioid and mesenchymal forms. Various types of epithelial or 
      myoepithelial malignancies arise from PA, but sarcomas are extremely rare. A 
      human androgen receptor gene (HUMARA) clonality assay has suggested that PA is 
      clonal in nature. However, clonality of various tumor components of PA would be 
      difficult to determine with this assay. In addition, the results obtained should 
      be carefully interpreted. PLAG1 rearrangements are considered a good molecular 
      marker for neoplasticity in PA. We aimed to clarify the neoplasticity of the 
      various tumor components present in PA using whole-slide fluorescence in situ 
      hybridization (FISH). Five PA cases positive for PLAG1 rearrangements were 
      examined. Using an immunohistochemistry panel, cell components in PA were 
      classified into eight cell types. To precisely localize PLAG1 
      rearrangement-positive cell components at the cellular level, sequential 
      retrieval of whole-slide imaging (WSI) data of HE histology and FISH for PLAG1 
      rearrangement was carried out. PLAG1 rearrangements were detected in ductal 
      cells, myoepithelial spindle cells, myoepithelial oncocytic cells, myoepithelial 
      plasmacytoid cells, and mesenchymal chondroid cells, but not in mesenchymal lipid 
      cells, mesenchymal fibrous cells, or vascular endothelial cells. 
      Immunohistochemical PLAG1 expression was restricted to cell components harboring 
      PLAG1 rearrangements.The results of the present study indicate that ductal and 
      myoepithelial, chondroid cells are neoplastic but lipid, fibrous, and endothelial 
      cells are not. PLAG1 immunohistochemistry is useful in discriminating neoplastic 
      from non-neoplastic cell components. These findings may be important for 
      elucidating tumorigenesis and the process of malignant transformation in PA.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Murase, Takayuki
AU  - Murase T
AD  - Department of Pathology and Molecular Diagnostics, Nagoya City University 
      Graduate School of Medical Sciences, Nagoya, 467-8601, Japan.
FAU - Ishibashi, Kenichiro
AU  - Ishibashi K
AD  - Department of Pathology and Molecular Diagnostics, Nagoya City University 
      Graduate School of Medical Sciences, Nagoya, 467-8601, Japan; Department of 
      Maxillofacial Surgery, Aichi-Gakuin University School of Dentistry, Nagoya, 
      467-8601, Japan.
FAU - Okumura, Yoshihide
AU  - Okumura Y
AD  - Department of Pathology and Molecular Diagnostics, Nagoya City University 
      Graduate School of Medical Sciences, Nagoya, 467-8601, Japan; Department of 
      Maxillofacial Surgery, Aichi-Gakuin University School of Dentistry, Nagoya, 
      467-8601, Japan.
FAU - Ueda, Kaori
AU  - Ueda K
AD  - Department of Pathology and Molecular Diagnostics, Nagoya City University 
      Graduate School of Medical Sciences, Nagoya, 467-8601, Japan; Department of 
      Maxillofacial Surgery, Aichi-Gakuin University School of Dentistry, Nagoya, 
      467-8601, Japan.
FAU - Nakano, Satsuki
AU  - Nakano S
AD  - Department of Pathology and Molecular Diagnostics, Nagoya City University 
      Graduate School of Medical Sciences, Nagoya, 467-8601, Japan.
FAU - Fujii, Keiichiro
AU  - Fujii K
AD  - Department of Pathology and Molecular Diagnostics, Nagoya City University 
      Graduate School of Medical Sciences, Nagoya, 467-8601, Japan.
FAU - Masaki, Ayako
AU  - Masaki A
AD  - Department of Pathology and Molecular Diagnostics, Nagoya City University 
      Graduate School of Medical Sciences, Nagoya, 467-8601, Japan.
FAU - Nagao, Toru
AU  - Nagao T
AD  - Department of Maxillofacial Surgery, Aichi-Gakuin University School of Dentistry, 
      Nagoya, 467-8601, Japan.
FAU - Inagaki, Hiroshi
AU  - Inagaki H
AD  - Department of Pathology and Molecular Diagnostics, Nagoya City University 
      Graduate School of Medical Sciences, Nagoya, 467-8601, Japan. Electronic address: 
      hinagaki@med.nagoya-cu.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211211
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Lipids)
RN  - 0 (PLAG1 protein, human)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - *Adenoma, Pleomorphic/diagnosis
MH  - Cell Transformation, Neoplastic/pathology
MH  - DNA-Binding Proteins/genetics
MH  - Endothelial Cells/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lipids
MH  - *Salivary Gland Neoplasms/diagnosis
MH  - Transcription Factors/genetics
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - Immunohistochemistry
OT  - PLAG1 rearrangements
OT  - Pleomorphic adenoma
OT  - Tumor components
OT  - Whole-slide imaging
EDAT- 2021/12/16 06:00
MHDA- 2022/05/10 06:00
CRDT- 2021/12/15 06:00
PHST- 2021/10/28 00:00 [received]
PHST- 2021/11/28 00:00 [revised]
PHST- 2021/11/29 00:00 [accepted]
PHST- 2021/12/16 06:00 [pubmed]
PHST- 2022/05/10 06:00 [medline]
PHST- 2021/12/15 06:00 [entrez]
AID - S0046-8177(21)00198-2 [pii]
AID - 10.1016/j.humpath.2021.11.010 [doi]
PST - ppublish
SO  - Hum Pathol. 2022 Feb;120:26-34. doi: 10.1016/j.humpath.2021.11.010. Epub 2021 Dec 
      11.