PMID- 34908216
OWN - NLM
STAT- MEDLINE
DCOM- 20220503
LR  - 20230202
IS  - 1600-0854 (Electronic)
IS  - 1398-9219 (Print)
IS  - 1398-9219 (Linking)
VI  - 23
IP  - 2
DP  - 2022 Feb
TI  - Bro1 binds the Vps20 subunit of ESCRT-III and promotes ESCRT-III regulation by 
      Doa4.
PG  - 109-119
LID - 10.1111/tra.12828 [doi]
AB  - The budding of intralumenal vesicles (ILVs) at endosomes requires membrane 
      scission by the ESCRT-III complex. This step is negatively regulated in yeast by 
      Doa4, the ubiquitin hydrolase that deubiquitinates transmembrane proteins sorted 
      as cargoes into ILVs. Doa4 acts non-enzymatically to inhibit ESCRT-III membrane 
      scission activity by directly binding the Snf7 subunit of ESCRT-III. This 
      interaction inhibits the remodeling/disassembly of Snf7 polymers required for the 
      ILV membrane scission reaction. Thus, Doa4 is thought to have a structural role 
      that delays ILV budding while it also functions enzymatically to deubiquitinate 
      ILV cargoes. In this study, we show that Doa4 binding to Snf7 in vivo is 
      antagonized by another ESCRT-III subunit, Vps20. Doa4 is restricted from 
      interacting with Snf7 in yeast expressing a mutant Vps20 allele that 
      constitutively binds Doa4. This inhibitory effect of Vps20 is suppressed by 
      overexpression of another ESCRT-III-associated protein, Bro1. We show that Bro1 
      binds directly to Vps20, suggesting that Bro1 has a central role in relieving the 
      antagonistic relationship that Vps20 has toward Doa4.
CI  - (c) 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Buysse, Dalton
AU  - Buysse D
AD  - Department of Molecular Cellular and Developmental Biology, University of 
      Colorado, Boulder, Colorado, USA.
FAU - West, Matt
AU  - West M
AD  - Department of Molecular Cellular and Developmental Biology, University of 
      Colorado, Boulder, Colorado, USA.
FAU - Leih, Mitchell
AU  - Leih M
AD  - Department of Molecular Cellular and Developmental Biology, University of 
      Colorado, Boulder, Colorado, USA.
FAU - Odorizzi, Greg
AU  - Odorizzi G
AUID- ORCID: 0000-0002-1143-1098
AD  - Department of Molecular Cellular and Developmental Biology, University of 
      Colorado, Boulder, Colorado, USA.
LA  - eng
GR  - R01 GM111335/GM/NIGMS NIH HHS/United States
GR  - T32 GM008759/GM/NIGMS NIH HHS/United States
GR  - T32 GM142607/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20220113
PL  - England
TA  - Traffic
JT  - Traffic (Copenhagen, Denmark)
JID - 100939340
RN  - 0 (Bro1 protein, S cerevisiae)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (Saccharomyces cerevisiae Proteins)
SB  - IM
MH  - *Endosomal Sorting Complexes Required for Transport/metabolism
MH  - Endosomes/metabolism
MH  - Intracellular Membranes/metabolism
MH  - Saccharomyces cerevisiae/metabolism
MH  - *Saccharomyces cerevisiae Proteins/metabolism
PMC - PMC8792227
MID - NIHMS1764596
OTO - NOTNLM
OT  - endosome
OT  - membrane scission
OT  - ubiquitin hydrolase
OT  - yeast
COIS- COMPETING INTERESTS The authors declare no competing interests.
EDAT- 2021/12/16 06:00
MHDA- 2022/05/04 06:00
PMCR- 2023/02/01
CRDT- 2021/12/15 09:04
PHST- 2021/11/19 00:00 [revised]
PHST- 2021/06/26 00:00 [received]
PHST- 2021/12/09 00:00 [accepted]
PHST- 2021/12/16 06:00 [pubmed]
PHST- 2022/05/04 06:00 [medline]
PHST- 2021/12/15 09:04 [entrez]
PHST- 2023/02/01 00:00 [pmc-release]
AID - 10.1111/tra.12828 [doi]
PST - ppublish
SO  - Traffic. 2022 Feb;23(2):109-119. doi: 10.1111/tra.12828. Epub 2022 Jan 13.