PMID- 34911016
OWN - NLM
STAT- MEDLINE
DCOM- 20220324
LR  - 20220324
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 229
DP  - 2022 Jan
TI  - LncRNA SNHG16 promotes proliferation and migration in laryngeal squamous cell 
      carcinoma via the miR-140-5p/NFAT5/Wnt/beta-catenin pathway axis.
PG  - 153727
LID - S0344-0338(21)00388-5 [pii]
LID - 10.1016/j.prp.2021.153727 [doi]
AB  - BACKGROUND: Recent studies demonstrate that long noncoding RNAs (lncRNAs) are 
      involved in the development of various cancers. Many lncRNAs were reported to 
      abnormally express in laryngeal squamous cell carcinoma (LSCC) and play pivotal 
      roles in its development. LncRNA small nucleolar RNA host gene 16 (SNHG16) was 
      previously validated as an oncogene in hepatocellular carcinoma. Nevertheless, 
      the biological role of SNHG16 in LSCC still needs more explorations. The goal of 
      this assay is to explore the function and molecular mechanism of lncRNA SNHG16 in 
      the development of LSCC. METHODS AND RESULTS: First, RT-qPCR demonstrated the 
      upregulation of SNHG16 in LSCC cells and tissues. Loss-of-function assays 
      determined the inhibitive influence of SNHG16 downregulation on cell viability, 
      growth, and migration in LSCC. Furthermore, SNHG16 bound with miR-140-5p in LSCC. 
      MiR-140-5p overexpression suppressed LSCC cell proliferation and migration. NFAT5 
      was identified as a direct target of miR-140-5p. Through rescue experiments, 
      overexpression of NFAT5 reversed SNHG16 knockdown-mediated suppression on cell 
      viability, growth, and migration in LSCC. Additionally, NFAT5 overexpression 
      activated while NFAT5 downregulation inhibited the Wnt/beta-catenin signaling 
      pathway. CONCLUSION: LncRNA SNHG16 is upregulated in LSCC and contributes to the 
      development of LSCC via regulating the miR-140-5p/NFAT5/Wnt/beta-catenin pathway 
      axis. The SNHG16/miR-140-5p/NFAT5/Wnt/beta-catenin pathway axis might provide a 
      novel strategy for LSCC treatment.
CI  - Copyright (c) 2021 Elsevier GmbH. All rights reserved.
FAU - Wan, Lanlan
AU  - Wan L
AD  - Department of Otolaryngology, The Affiliated Huaian No.1 People's Hospital of 
      Nanjing Medical University, Huaian 223300, Jiangsu, China.
FAU - Gu, Dongsheng
AU  - Gu D
AD  - Department of Otolaryngology, The Affiliated Huaian No.1 People's Hospital of 
      Nanjing Medical University, Huaian 223300, Jiangsu, China.
FAU - Li, Peizhong
AU  - Li P
AD  - Department of Otolaryngology, The Affiliated Huaian No.1 People's Hospital of 
      Nanjing Medical University, Huaian 223300, Jiangsu, China. Electronic address: 
      madhatter6555@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20211128
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn140 microRNA, human)
RN  - 0 (NFAT5 protein, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (SNHG16 lncRNA, human)
RN  - 0 (Transcription Factors)
RN  - 0 (beta Catenin)
SB  - IM
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Down-Regulation
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*pathology
MH  - Male
MH  - MicroRNAs/*physiology
MH  - Middle Aged
MH  - RNA, Long Noncoding/*physiology
MH  - Squamous Cell Carcinoma of Head and Neck/*pathology
MH  - Transcription Factors/*physiology
MH  - Up-Regulation
MH  - Wnt Signaling Pathway/*physiology
MH  - beta Catenin/*physiology
OTO - NOTNLM
OT  - Laryngeal squamous cell carcinoma
OT  - MiR-140-5p
OT  - NFAT5
OT  - SNHG16
OT  - The Wnt/beta-catenin signaling pathway
EDAT- 2021/12/16 06:00
MHDA- 2022/03/25 06:00
CRDT- 2021/12/15 20:12
PHST- 2021/06/30 00:00 [received]
PHST- 2021/11/10 00:00 [revised]
PHST- 2021/11/26 00:00 [accepted]
PHST- 2021/12/16 06:00 [pubmed]
PHST- 2022/03/25 06:00 [medline]
PHST- 2021/12/15 20:12 [entrez]
AID - S0344-0338(21)00388-5 [pii]
AID - 10.1016/j.prp.2021.153727 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2022 Jan;229:153727. doi: 10.1016/j.prp.2021.153727. Epub 2021 
      Nov 28.