PMID- 34911834
OWN - NLM
STAT- MEDLINE
DCOM- 20211217
LR  - 20240321
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 46
IP  - 10
DP  - 2021 Oct 28
TI  - Impact of microRNA-21-5p on the growth of thyroid cancer cells via targeting the 
      recombinant sclerostin domain containing protein 1.
PG  - 1054-1062
LID - 1672-7347(2021)10-1054-09 [pii]
LID - 10.11817/j.issn.1672-7347.2021.200764 [doi]
AB  - OBJECTIVES: To explore the molecular mechanism for thyroid cancer metastasis via 
      analyzing the role of microRNA (miR)-21-5p and its target gene recombinant 
      sclerostin domain containing protein 1 (SOSTDC1) in thyroid cancer. METHODS: The 
      target miR-21-5p was screened through bioinformatics analysis and cell 
      verification, and the thyroid cancer cell lines was transfected with miR-21-5p 
      inhibitor. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide 
      (MTT) test, flow cytometry, and cell scratch test were used to detect the 
      proliferation, apoptosis and migration of thyroid cancer cells in the miR-21-5p 
      inhibitor group and the inhibitor control group, respectively. The luciferase 
      report experiment was used to verify the relationship between miR-21-5p and 
      SOSTDC1, Western blotting was used to detect the expression levels and 
      phosphorylation levels of SOSTDC1,phosphatidylinositol 3 kinase (PI3K), protein 
      kinase B (Akt) and mitogen-activated protein kinases (MAPK), extracellular 
      regulated protein kinases (ERK) in thyroid cancer cells. RESULTS: MiR-21-5p was 
      significantly increased in thyroid cancer cells,which was negatively correlated 
      with SOSTDC1 (r=-0.24, P<0.01). The proliferation and migration of thyroid cancer 
      cells in the miR-21-5p inhibitor group was significantly lower than that in the 
      inhibitor control group (both P<0.01), and the apoptosis rate in the miR-21-5p 
      inhibitor group was significantly higher than that in the inhibitor control group 
      (P<0.01).The luciferase report experiment showed that miR-21-5p could target and 
      regulate the expression level of SOSTDC1, and the expression of PI3K in the 
      miR-21-5p inhibitor group was significantly lower than that in the inhibitor 
      control group (P<0.01). There were no significant changes in Akt and ERK1/2 
      levels, but the phosphorylation levels of Akt and ERK1/2 in the miR-21-5p 
      inhibitor group were significantly lower than those in the inhibitor control 
      group (both P<0.01). CONCLUSIONS: MiR-21-5p in thyroid cancer cells can target 
      the expression of SOSTDC1 and affect the activities of PI3K/Akt and MAPK/ERK, 
      thereby inhibiting the apoptosis of thyroid cancer cells and promoting cell 
      proliferation and migration.
FAU - Liu, Miaomiao
AU  - Liu M
AD  - Department of Nuclear Medicine, Xiangya Hospital, Central South University, 
      Changsha 410008, China. liumm0527@163.com.
FAU - Deng, Haoyu
AU  - Deng H
AD  - Department of Nuclear Medicine, Xiangya Hospital, Central South University, 
      Changsha 410008, China.
FAU - Zhao, Yajie
AU  - Zhao Y
AD  - Department of Nuclear Medicine, Xiangya Hospital, Central South University, 
      Changsha 410008, China.
FAU - Li, Can
AU  - Li C
AD  - Department of Nuclear Medicine, Xiangya Hospital, Central South University, 
      Changsha 410008, China.
FAU - Liu, Hua
AU  - Liu H
AD  - Department of Nuclear Medicine, Xiangya Hospital, Central South University, 
      Changsha 410008, China. liuhua23@csu.edu.cn.
LA  - eng
LA  - chi
PT  - Journal Article
TT  - 微RNA-21-5p靶向调控含硬化蛋白域蛋白1对甲状腺癌细胞生长的影响.
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (SOSTDC1 protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Apoptosis/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Humans
MH  - *MicroRNAs/genetics
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/genetics/metabolism
MH  - *Thyroid Neoplasms/genetics
PMC - PMC10930239
OAB - OBJECTIVE: To explore the molecular mechanism for thyroid cancer metastasis via 
      analyzing the role of microRNA (miR)-21-5p and its target gene recombinant 
      sclerostin domain containing protein 1 (SOSTDC1) in thyroid cancer. METHODS: The 
      target miR-21-5p was screened through bioinformatics analysis and cell 
      verification, and the thyroid cancer cell lines was transfected with miR-21-5p 
      inhibitor. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide 
      (MTT) test, flow cytometry, and cell scratch test were used to detect the 
      proliferation, apoptosis and migration of thyroid cancer cells in the miR-21-5p 
      inhibitor group and the inhibitor control group, respectively. The luciferase 
      report experiment was used to verify the relationship between miR-21-5p and 
      SOSTDC1, Western blotting was used to detect the expression levels and 
      phosphorylation levels of SOSTDC1,phosphatidylinositol 3 kinase (PI3K), protein 
      kinase B (Akt) and mitogen-activated protein kinases (MAPK), extracellular 
      regulated protein kinases (ERK) in thyroid cancer cells. RESULTS: MiR-21-5p was 
      significantly increased in thyroid cancer cells,which was negatively correlated 
      with SOSTDC1 (r=-0.24, P<0.01). The proliferation and migration of thyroid cancer 
      cells in the miR-21-5p inhibitor group was significantly lower than that in the 
      inhibitor control group (both P<0.01), and the apoptosis rate in the miR-21-5p 
      inhibitor group was significantly higher than that in the inhibitor control group 
      (P<0.01).The luciferase report experiment showed that miR-21-5p could target and 
      regulate the expression level of SOSTDC1, and the expression of PI3K in the 
      miR-21-5p inhibitor group was significantly lower than that in the inhibitor 
      control group (P<0.01). There were no significant changes in Akt and ERK1/2 
      levels, but the phosphorylation levels of Akt and ERK1/2 in the miR-21-5p 
      inhibitor group were significantly lower than those in the inhibitor control 
      group (both P<0.01). CONCLUSION: MiR-21-5p in thyroid cancer cells can target the 
      expression of SOSTDC1 and affect the activities of PI3K/Akt and MAPK/ERK, thereby 
      inhibiting the apoptosis of thyroid cancer cells and promoting cell proliferation 
      and migration.
OABL- eng
OTO - NOTNLM
OT  - microRNA-21-5p
OT  - recombinant sclerostin domain containing protein 1
OT  - thyroid cancer
COIS- 作者声称无任何利益冲突。
EDAT- 2021/12/17 06:00
MHDA- 2021/12/18 06:00
PMCR- 2021/10/28
CRDT- 2021/12/16 05:53
PHST- 2021/12/16 05:53 [entrez]
PHST- 2021/12/17 06:00 [pubmed]
PHST- 2021/12/18 06:00 [medline]
PHST- 2021/10/28 00:00 [pmc-release]
AID - 1672-7347(2021)10-1054-09 [pii]
AID - 10.11817/j.issn.1672-7347.2021.200764 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2021 Oct 28;46(10):1054-1062. doi: 
      10.11817/j.issn.1672-7347.2021.200764.