PMID- 34914199
OWN - NLM
STAT- MEDLINE
DCOM- 20220421
LR  - 20220802
IS  - 2053-1095 (Electronic)
IS  - 2053-1095 (Linking)
VI  - 8
IP  - 2
DP  - 2022 Mar
TI  - Pre-treatment of ram semen extender with magnetic nanoparticles on freeze-thawed 
      spermatozoa.
PG  - 792-798
LID - 10.1002/vms3.689 [doi]
AB  - BACKGROUND: Extensive use of different nanoparticles caused significant concerns 
      about their biological safety. OBJECTIVE: This study aimed to evaluate the 
      effects of cryopreservation on ram semen after adding magnetic nanoparticles 
      (MNPs) to separate X and Y chromosome-bearing spermatozoa. METHODS: The 
      experimental ram sperms in this research included treated spermatozoa (50 mug/ml 
      MNPs) and non-treated spermatozoa. DNA damage of spermatozoa was examined using 
      an acridine orange (AO) assay. Sperm viability, membrane functionality, 
      abnormality and malondialdehyde (MDA) level were also measured. RESULTS: Results 
      indicated that the pre-treatment of ram semen extender with MNPs did not 
      significantly affect the semen parameters such as viability, membrane 
      functionality, abnormality, as well as lipid peroxidation (LPO) levels and DNA 
      integrity in comparison with the control group (p < 0.05). CONCLUSIONS: These 
      observations suggest that pre-treatment of ram semen extender with MNPs after 
      semen sexing did not have adverse effects on different semen parameters after 
      cryopreservation.
CI  - (c) 2021 The Authors. Veterinary Medicine and Science published by John Wiley & 
      Sons Ltd.
FAU - Moradi, Maryam
AU  - Moradi M
AD  - Faculty of Agricultural and Engineering Science, Department of Animal Science, 
      Razi University, Kermanshah, Iran.
FAU - Hajarian, Hadi
AU  - Hajarian H
AUID- ORCID: 0000-0003-0738-429X
AD  - Faculty of Agricultural and Engineering Science, Department of Animal Science, 
      Razi University, Kermanshah, Iran.
FAU - Karamishabankareh, Hamed
AU  - Karamishabankareh H
AUID- ORCID: 0000-0002-4810-2415
AD  - Faculty of Agricultural and Engineering Science, Department of Animal Science, 
      Razi University, Kermanshah, Iran.
FAU - Soltani, Leila
AU  - Soltani L
AUID- ORCID: 0000-0002-5007-5110
AD  - Faculty of Agricultural and Engineering Science, Department of Animal Science, 
      Razi University, Kermanshah, Iran.
FAU - Soleymani, Bijan
AU  - Soleymani B
AUID- ORCID: 0000-0003-3059-413X
AD  - Medical Biology Research Center, Health Technology Institute, Kermanshah 
      University of Medical Science, Kermanshah, Iran.
LA  - eng
PT  - Journal Article
DEP - 20211216
PL  - England
TA  - Vet Med Sci
JT  - Veterinary medicine and science
JID - 101678837
RN  - 0 (Cryoprotective Agents)
RN  - 0 (Magnetite Nanoparticles)
SB  - IM
MH  - Animals
MH  - Cryoprotective Agents/pharmacology
MH  - *Magnetite Nanoparticles
MH  - Male
MH  - *Semen Preservation/methods/veterinary
MH  - Sheep
MH  - Sperm Motility
MH  - Spermatozoa
PMC - PMC8959327
OTO - NOTNLM
OT  - cryopreservation
OT  - magnetic nanoparticles
OT  - ram
OT  - semen
OT  - sperm morphology
COIS- The author declares that they have no conflict of interest.
EDAT- 2021/12/17 06:00
MHDA- 2022/04/22 06:00
PMCR- 2021/12/16
CRDT- 2021/12/16 12:43
PHST- 2021/12/17 06:00 [pubmed]
PHST- 2022/04/22 06:00 [medline]
PHST- 2021/12/16 12:43 [entrez]
PHST- 2021/12/16 00:00 [pmc-release]
AID - VMS3689 [pii]
AID - 10.1002/vms3.689 [doi]
PST - ppublish
SO  - Vet Med Sci. 2022 Mar;8(2):792-798. doi: 10.1002/vms3.689. Epub 2021 Dec 16.