PMID- 34914327
OWN - NLM
STAT- MEDLINE
DCOM- 20211220
LR  - 20220429
IS  - 1009-3591 (Print)
IS  - 1009-3591 (Linking)
VI  - 27
IP  - 2
DP  - 2021 Feb
TI  - [Standardization and quality control for detection of sperm DNA damage by flow 
      cytometry: A preliminary investigation].
PG  - 124-128
AB  - OBJECTIVE: To investigate preliminarily the standardization and quality control 
      for the detection of sperm DNA damage by flow cytometry. METHODS: Semen samples 
      were randomly selected and observed for the effects of acid denaturation time, 
      acridine orange (AO) staining time, semen sample refrigeration, freezing and 
      repeated freezing-thawing on the results of sperm DNA fragmentation index (DFI). 
      RESULTS: Sperm DFI increased gradually with the prolongation of acid denaturation 
      time, significantly at 2 minutes in comparison with that at 30 seconds (P<0.05). 
      There was no significant difference in sperm DFI after AO staining for 5, 20, 40, 
      60, 100 and 140 minutes. Sperm DFI also exhibited an evident increase with the 
      prolongation of the refrigeration of the semen samples at 2℃－8℃, significantly at 
      2 days. The semen samples could be frozen directly at －20℃, and three times of 
      repeated freezing and thawing produced little effect on the results of sperm DFI, 
      except for some inadequate stability. Based on the data obtained from freezing 
      the semen samples after sub-packed and tested 2 days for 1 month and simulation 
      of inter-laboratory quality control, the calculated CV value was 7.13%. 
      CONCLUSIONS: In detection of sperm DFI, it is very important to ensure the 
      accuracy of acid denaturation time, which cannot exceed 1 minute at most. The 
      time of or after AO staining does not significantly affect the results of sperm 
      DFI. The samples for detection of sperm DFI should be fresh and not exceed 1 day 
      in case of refrigeration. Directly frozen semen samples can be used as the 
      materials for inter-laboratory quality control for detection of sperm DFI. 
      Whether cryoprotectants can make frozen semen samples more stable and how to 
      prepare and transport the materials for inter-laboratory quality control are the 
      key problems to be solved in the future.
FAU - Lu, Jin-Chun
AU  - Lu JC
AD  - Center for Reproductive Medicine, Zhongda Hospital, Southeast University, 
      Nanjing, Jiangsu 210037, China.
FAU - Wu, Zhen-Bo
AU  - Wu ZB
AD  - Nanjing Xindi Biopharmaceutical Engineering Co., Ltd., Nanjing, Jiangsu 211112, 
      China.
FAU - Tang, Shan-Shan
AU  - Tang SS
AD  - Center for Reproductive Medicine, Zhongda Hospital, Southeast University, 
      Nanjing, Jiangsu 210037, China.
FAU - Xu, Yuan-Hua
AU  - Xu YH
AD  - Center for Reproductive Medicine, Zhongda Hospital, Southeast University, 
      Nanjing, Jiangsu 210037, China.
FAU - Liu, Yuan-Yuan
AU  - Liu YY
AD  - Nanjing Xindi Biopharmaceutical Engineering Co., Ltd., Nanjing, Jiangsu 211112, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Nan Ke Xue
JT  - Zhonghua nan ke xue = National journal of andrology
JID - 101093592
SB  - IM
MH  - *DNA Damage
MH  - Flow Cytometry
MH  - Humans
MH  - Male
MH  - Quality Control
MH  - Reference Standards
MH  - *Spermatozoa
OTO - NOTNLM
OT  - sperm DNA damage
OT  - DNA fragmentation index
OT  - flow cytometry
OT  - quality control
OT  - standardization
EDAT- 2021/12/17 06:00
MHDA- 2021/12/21 06:00
CRDT- 2021/12/16 12:44
PHST- 2021/12/16 12:44 [entrez]
PHST- 2021/12/17 06:00 [pubmed]
PHST- 2021/12/21 06:00 [medline]
PST - ppublish
SO  - Zhonghua Nan Ke Xue. 2021 Feb;27(2):124-128.