PMID- 34915068
OWN - NLM
STAT- MEDLINE
DCOM- 20220209
LR  - 20220209
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 192
DP  - 2022 Jan
TI  - Bioremediation of oily seawater by Bacteria immobilization on a novel carrier 
      material containing nutrients.
PG  - 106392
LID - S0167-7012(21)00260-8 [pii]
LID - 10.1016/j.mimet.2021.106392 [doi]
AB  - A novel carrier material was obtained by coating puffed rhubarb rice (PRR) with 
      calcium alginate (CA) membrane. The carrier material was prepared to contain 
      oil-degrading bacterial strains and inorganic nutrients through entrapping them 
      in different locations. This formulation possessed floatability, biodegradability 
      and nutrient slow-release properties. Therefore, it could be applied for oil 
      biodegradation on seawater surfaces. For controlling the release rate of 
      nutrients, the optimal preparation technique was established. The number of 
      viable cells immobilized on the carrier material reached 2 x 10(9) CFU/g. This 
      formulation could be stored at -20  degrees C for three months without a significant 
      decrease in the number of viable immobilized cells (4 x 10(8) CFU/g). Scanning 
      electron microscope (SEM) results showed that the cells were immobilized on the 
      outer CA membrane, and the inorganic nutrients were entrapped in the inner PRR 
      and CA membrane. The immobilized cells were able to remove 86% of the diesel oil 
      at an initial diesel oil concentration of 1% (v/v), an incubation temperature of 
      37  degrees C, during three days of incubation. Gas chromatography-mass spectrometry 
      (GC-MS) analysis results showed that most components of diesel oil were degraded 
      by the formulations.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Luo, Qun
AU  - Luo Q
AD  - Naval Medical Center of Chinese People's Liberation Army, Shanghai 200433, China.
FAU - Liu, Lina
AU  - Liu L
AD  - Naval Medical Center of Chinese People's Liberation Army, Shanghai 200433, China.
FAU - Hou, Dengyong
AU  - Hou D
AD  - Naval Medical Center of Chinese People's Liberation Army, Shanghai 200433, China. 
      Electronic address: Houdy2@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211213
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Fuel Oils)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Bacteria/*metabolism
MH  - *Biodegradation, Environmental
MH  - Cells, Immobilized/metabolism
MH  - Fuel Oils/*analysis
MH  - Gas Chromatography-Mass Spectrometry
MH  - Seawater/*chemistry/microbiology
MH  - Water Pollutants, Chemical/*analysis
OTO - NOTNLM
OT  - Bioremediation
OT  - Carrier material
OT  - Immobilization
OT  - Inorganic nutrient
OT  - Seawater
EDAT- 2021/12/17 06:00
MHDA- 2022/02/10 06:00
CRDT- 2021/12/16 20:18
PHST- 2021/06/21 00:00 [received]
PHST- 2021/12/05 00:00 [revised]
PHST- 2021/12/09 00:00 [accepted]
PHST- 2021/12/17 06:00 [pubmed]
PHST- 2022/02/10 06:00 [medline]
PHST- 2021/12/16 20:18 [entrez]
AID - S0167-7012(21)00260-8 [pii]
AID - 10.1016/j.mimet.2021.106392 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2022 Jan;192:106392. doi: 10.1016/j.mimet.2021.106392. Epub 
      2021 Dec 13.