PMID- 34916691
OWN - NLM
STAT- MEDLINE
DCOM- 20211220
LR  - 20220105
IS  - 1671-167X (Print)
IS  - 1671-167X (Linking)
VI  - 53
IP  - 6
DP  - 2021 Dec 18
TI  - [Characteristics of gastric microbiota in children with Helicobacter pylori 
      infection family history].
PG  - 1115-1121
AB  - OBJECTIVE: To explore the characteristics of gastric microbiota in children with 
      and without (Helicobacter pylori, H. pylori) infection who had family history of 
      H. pylori infection. METHODS: Mucosal biopsy samples of the gastric corpus and 
      gastric antrum were collected during the gastroscope. And the gastric mucosa 
      flora's information of the two groups of children were obtained after sample DNA 
      extraction, PCR amplification of the 16S ribosomal DNA (rDNA) V3-V4 region, 
      high-throughput sequencing and data processing. All the samples with family 
      history of H. pylori infection were divided into two groups, the H. pylori 
      infection group (n=18) and the H. pylori non-infection group (n=24). Then the alpha-, 
      beta-diversity and bacteria abundance of the gastric microbiota were compared 
      between the H. pylori infection and non-infection groups at different taxonomic 
      levels. The differential microbiota was found out by LEfSe analysis, and then the 
      function of microbiota predicted using phylogenetic investigation of communities 
      by reconstruction of unobserved states (PICRUSt) method. RESULTS: There was 
      statistically significant difference in alpha-diversity (P < 0.05) between the two 
      groups, indicating that the H. pylori non-infection group had higher microbial 
      richness than the H. pylori infection group. Moreover, the beta-diversity was 
      significantly different as well (P < 0.05), which meant that the microbiota 
      composition of the two groups was different. At the phyla level, Proteobacteria, 
      Firmicutes, Bacteroides, Actinobacteria, and Fusobacteria were dominant in the 
      two groups. At the genus level, Bacteroides, Prevotella, Streptococcus, and 
      Neisseria, etc. were dominant in the H. pylori non-infected group. Meanwhile, 
      Helicobacter and Haemophilus etc. were dominant in the H. pylori infected group. 
      LEfSe analysis showed that the relative abundance of Bacteroides etc. at the 
      genus level in the H. pylori non-infected group was significantly higher than 
      that in the H. pylori infected group. Functional prediction showed that 
      Bacteroides were positively correlated with amino acid and vitamin metabolism, 
      mitogen-activated protein kinase (MAPK), mammalian target of rapamycin (mTOR) 
      signaling pathway and ansamycin synthesis pathway. CONCLUSION: The gastric 
      microbiota between H. pylori positive and H. pylori negative in children with 
      family history of H. pylori infection is significant different. Some gastric 
      microbiota, such as Bacteroides, may have a potential relationship with H. pylori 
      infection in children.
FAU - Wang, Z J
AU  - Wang ZJ
AD  - Department of Pediatric, Peking University Third Hospital, Beijing 100191, China.
FAU - Li, Z L
AU  - Li ZL
AD  - Department of Pediatric, Peking University Third Hospital, Beijing 100191, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Beijing Da Xue Xue Bao Yi Xue Ban
JT  - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health 
      sciences
JID - 101125284
SB  - IM
MH  - Child
MH  - Gastric Mucosa
MH  - *Helicobacter Infections
MH  - *Helicobacter pylori
MH  - Humans
MH  - *Microbiota
MH  - Phylogeny
PMC - PMC8695169
OTO - NOTNLM
OT  - Child
OT  - Gastrointestinal microbiome
OT  - Helicobacter pylori
OT  - Medical history taking
EDAT- 2021/12/18 06:00
MHDA- 2021/12/21 06:00
PMCR- 2021/12/18
CRDT- 2021/12/17 06:49
PHST- 2021/12/17 06:49 [entrez]
PHST- 2021/12/18 06:00 [pubmed]
PHST- 2021/12/21 06:00 [medline]
PHST- 2021/12/18 00:00 [pmc-release]
AID - bjdxxbyxb-53-6-1115 [pii]
AID - 10.19723/j.issn.1671-167X.2021.06.018 [doi]
PST - ppublish
SO  - Beijing Da Xue Xue Bao Yi Xue Ban. 2021 Dec 18;53(6):1115-1121. doi: 
      10.19723/j.issn.1671-167X.2021.06.018.