PMID- 34917610
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211218
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 9
DP  - 2021
TI  - Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in 
      Porcine Oocytes.
PG  - 733860
LID - 10.3389/fcell.2021.733860 [doi]
LID - 733860
AB  - The endoplasmic reticulum (ER) is a multifunctional organelle in the cytoplasm 
      that plays important roles in female mammalian reproduction. The endoplasmic 
      reticulum and mitochondria interact to maintain the normal function of cells by 
      maintaining intracellular calcium homeostasis. As proven by previous research, 
      glycine (Gly) can regulate the intracellular free calcium concentration 
      ([Ca(2+)](i)) and enhance mitochondrial function to improve oocyte maturation in 
      vitro. The effect of Gly on ER function during oocyte in vitro maturation (IVM) 
      is not clear. In this study, we induced an ER stress model with thapsigargin (TG) 
      to explore whether Gly can reverse the ER stress induced by TG treatment and 
      whether it is associated with calcium regulation. The results showed that the 
      addition of Gly could improve the decrease in the average cumulus diameter, the 
      first polar body excretion rate caused by TG-induced ER stress, the cleavage rate 
      and the blastocyst rate. Gly supplementation could reduce the ER stress induced 
      by TG by significantly improving the ER levels and significantly downregulating 
      the expression of genes related to ER stress (Xbp1, ATF4, and ATF6). Moreover, 
      Gly also significantly alleviated the increase in reactive oxygen species (ROS) 
      levels and the decrease in mitochondrial membrane potential (DeltaPsi m) to improve 
      mitochondrial function in porcine oocytes exposed to TG. Furthermore, Gly reduced 
      the [Ca(2+)](i) and mitochondrial Ca(2+) ([Ca(2+)](m)) levels and restored the ER 
      Ca(2+) ([Ca(2+)](ER)) levels in TG-exposed porcine oocytes. Moreover, we found 
      that the increase in [Ca(2+)](i) may be caused by changes in the distribution and 
      expression of inositol 1,4,5-triphosphate receptor (IP(3)R1) and 
      voltage-dependent anion channel 1 (VDAC1), while Gly can restore the distribution 
      and expression of IP(3)R1 and VDAC1 to normal levels. Apoptosis-related indexes 
      (Caspase 3 activity and Annexin-V) and gene expression Bax, Cyto C, and Caspase 
      3) were significantly increased in the TG group, but they could be restored by 
      adding Gly. Our results suggest that Gly can ameliorate ER stress and apoptosis 
      in TG-exposed porcine oocytes and can further enhance the developmental potential 
      of porcine oocytes in vitro.
CI  - Copyright (c) 2021 Yu, Gao, Zhang, Wang, Lan, Chu, Li and Zheng.
FAU - Yu, Sicong
AU  - Yu S
AD  - College of Animal Science and Technology, Jilin Agricultural University, 
      Changchun, China.
FAU - Gao, Lepeng
AU  - Gao L
AD  - College of Animal Science and Technology, Jilin Agricultural University, 
      Changchun, China.
FAU - Zhang, Chang
AU  - Zhang C
AD  - College of Animal Science and Technology, Jilin Agricultural University, 
      Changchun, China.
FAU - Wang, Yumeng
AU  - Wang Y
AD  - College of Animal Science and Technology, Jilin Agricultural University, 
      Changchun, China.
FAU - Lan, Hainan
AU  - Lan H
AD  - College of Animal Science and Technology, Jilin Agricultural University, 
      Changchun, China.
FAU - Chu, Qianran
AU  - Chu Q
AD  - College of Animal Science and Technology, Jilin Agricultural University, 
      Changchun, China.
FAU - Li, Suo
AU  - Li S
AD  - College of Animal Science and Technology, Jilin Agricultural University, 
      Changchun, China.
FAU - Zheng, Xin
AU  - Zheng X
AD  - College of Animal Science and Technology, Jilin Agricultural University, 
      Changchun, China.
LA  - eng
PT  - Journal Article
DEP - 20211130
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC8670231
OTO - NOTNLM
OT  - apoptosis
OT  - embryo development (in vitro)
OT  - endoplasmic reticulum stress
OT  - glycine
OT  - oocyte meiotic maturation
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/12/18 06:00
MHDA- 2021/12/18 06:01
PMCR- 2021/01/01
CRDT- 2021/12/17 06:59
PHST- 2021/06/30 00:00 [received]
PHST- 2021/11/08 00:00 [accepted]
PHST- 2021/12/17 06:59 [entrez]
PHST- 2021/12/18 06:00 [pubmed]
PHST- 2021/12/18 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 733860 [pii]
AID - 10.3389/fcell.2021.733860 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2021 Nov 30;9:733860. doi: 10.3389/fcell.2021.733860. 
      eCollection 2021.