PMID- 34917981
OWN - NLM
STAT- MEDLINE
DCOM- 20220330
LR  - 20220716
IS  - 2666-1667 (Electronic)
IS  - 2666-1667 (Linking)
VI  - 2
IP  - 4
DP  - 2021 Dec 17
TI  - A protocol for macrophage depletion and reconstitution in a mouse model of 
      sepsis.
PG  - 101004
LID - 10.1016/j.xpro.2021.101004 [doi]
LID - 101004
AB  - Macrophages are key innate immune cells involved in a broad spectrum of 
      physiological and pathological processes. Macrophage depletion with 
      clodronate-liposomes is commonly used to investigate in vivo functions of 
      macrophages in mice. Here, we describe a protocol that combines the depletion of 
      resident macrophages with the reconstitution of the mice with in vitro 
      differentiated, lentivirus-transduced bone marrow-derived macrophages (BMDMs) in 
      the context of an experimental sepsis model. This experimental strategy is easily 
      adapted to other experimental designs. For complete details on the use and 
      execution of this protocol, please refer to Du et al. (2020).
CI  - (c) 2021 The Author(s).
FAU - Nguyen, Tivoli
AU  - Nguyen T
AD  - Department of Medicine, Division of Biological Sciences, The University of 
      Chicago, Chicago, IL, USA.
FAU - Du, Jie
AU  - Du J
AD  - Department of Medicine, Division of Biological Sciences, The University of 
      Chicago, Chicago, IL, USA.
AD  - Department of Oral Medicine, School and Hospital of Stomatology, Shanxi Medical 
      University, Taiyuan, Shanxi, China.
FAU - Li, Yan Chun
AU  - Li YC
AD  - Department of Medicine, Division of Biological Sciences, The University of 
      Chicago, Chicago, IL, USA.
LA  - eng
GR  - R01 AI151162/AI/NIAID NIH HHS/United States
GR  - R21 AI140152/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20211208
PL  - United States
TA  - STAR Protoc
JT  - STAR protocols
JID - 101769501
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - *Disease Models, Animal
MH  - Immunosuppression Therapy
MH  - Lentivirus/genetics
MH  - *Macrophages/cytology/immunology/transplantation
MH  - Mice
MH  - Sepsis/*immunology
PMC - PMC8669096
OTO - NOTNLM
OT  - Cell Biology
OT  - Cell Differentiation
OT  - Flow Cytometry/Mass Cytometry
OT  - Immunology
OT  - Model Organisms
COIS- The authors declare no competing interests.
EDAT- 2021/12/18 06:00
MHDA- 2022/03/31 06:00
PMCR- 2021/12/08
CRDT- 2021/12/17 07:04
PHST- 2021/12/17 07:04 [entrez]
PHST- 2021/12/18 06:00 [pubmed]
PHST- 2022/03/31 06:00 [medline]
PHST- 2021/12/08 00:00 [pmc-release]
AID - S2666-1667(21)00710-3 [pii]
AID - 101004 [pii]
AID - 10.1016/j.xpro.2021.101004 [doi]
PST - epublish
SO  - STAR Protoc. 2021 Dec 8;2(4):101004. doi: 10.1016/j.xpro.2021.101004. eCollection 
      2021 Dec 17.