PMID- 34918065
OWN - NLM
STAT- MEDLINE
DCOM- 20220217
LR  - 20220217
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Linking)
VI  - 163
IP  - 2
DP  - 2022 Feb 1
TI  - CircRNA-WNK2 Acts as a ceRNA for miR-328a-3p to Promote AANAT Expression in the 
      Male Rat Pineal Gland.
LID - bqab255 [pii]
LID - 10.1210/endocr/bqab255 [doi]
AB  - Noncoding RNAs (ncRNAs), including microRNAs (miRNAs) and circular RNAs 
      (circRNAs), which are expressed with a daily rhythm in the rat pineal gland, are 
      associated with the regulation of melatonin secretion and other biological 
      functions. However, the mechanisms of these molecules in the rat pineal gland are 
      not yet fully understood. In this study, we found that circR-WNK2 was highly 
      expressed at night, which may be involved in the regulation of melatonin 
      secretion through the competitive endogenous RNA (ceRNA) mechanism. By dual 
      luciferase reporter, RNA pull-down, and fluorescence in situ hybridization (FISH) 
      assays, we found that miR-328a-3p can target circR-WNK2 and the Aa-nat mRNA 
      3'UTR. Transfection experiments indicated that circR-WNK2 could competitively 
      bind to miR-328a-3p, reduce miR-328a-3p expression, and promote Aa-nat gene 
      expression and melatonin secretion. And by constructing a superior cervical 
      ganglionectomy (SCGx) rat model, we found that ncRNAs expression in the pineal 
      gland was regulated by signals from the suprachiasmatic nucleus. This finding 
      supports the hypothesis that these noncoding RNAs may interact to shape the 
      circadian rhythm through transcriptional processing in melatonin synthesis.
CI  - (c) The Author(s) 2021. Published by Oxford University Press on behalf of the 
      Endocrine Society. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Zheng, Yi
AU  - Zheng Y
AD  - Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal 
      Model, Jilin University, Changchun 130062, Jilin, P.R. China.
FAU - Wang, Hao-Qi
AU  - Wang HQ
AD  - Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal 
      Model, Jilin University, Changchun 130062, Jilin, P.R. China.
FAU - Guo, Hai-Xiang
AU  - Guo HX
AD  - Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal 
      Model, Jilin University, Changchun 130062, Jilin, P.R. China.
FAU - Xie, Heng-Li
AU  - Xie HL
AD  - Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal 
      Model, Jilin University, Changchun 130062, Jilin, P.R. China.
FAU - Zhang, Wei-Di
AU  - Zhang WD
AD  - Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal 
      Model, Jilin University, Changchun 130062, Jilin, P.R. China.
FAU - Han, Dong-Xu
AU  - Han DX
AD  - Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal 
      Model, Jilin University, Changchun 130062, Jilin, P.R. China.
FAU - Jiang, Hao
AU  - Jiang H
AUID- ORCID: 0000-0003-2008-6166
AD  - Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal 
      Model, Jilin University, Changchun 130062, Jilin, P.R. China.
FAU - Yuan, Bao
AU  - Yuan B
AUID- ORCID: 0000-0003-3490-0755
AD  - Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal 
      Model, Jilin University, Changchun 130062, Jilin, P.R. China.
FAU - Zhang, Jia-Bao
AU  - Zhang JB
AUID- ORCID: 0000-0001-8533-4744
AD  - Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal 
      Model, Jilin University, Changchun 130062, Jilin, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (MIRN328 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Untranslated)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Circadian Rhythm/genetics
MH  - Gene Expression
MH  - Male
MH  - Melatonin/*genetics/metabolism
MH  - MicroRNAs/*genetics
MH  - Models, Animal
MH  - Pineal Gland/*metabolism
MH  - Protein Serine-Threonine Kinases/*genetics
MH  - RNA, Untranslated/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Suprachiasmatic Nucleus/metabolism
MH  - Transcription, Genetic
OTO - NOTNLM
OT  - circular RNAs
OT  - melatonin secretion
OT  - rat pineal
EDAT- 2021/12/18 06:00
MHDA- 2022/02/19 06:00
CRDT- 2021/12/17 07:08
PHST- 2021/06/21 00:00 [received]
PHST- 2021/12/18 06:00 [pubmed]
PHST- 2022/02/19 06:00 [medline]
PHST- 2021/12/17 07:08 [entrez]
AID - 6464229 [pii]
AID - 10.1210/endocr/bqab255 [doi]
PST - ppublish
SO  - Endocrinology. 2022 Feb 1;163(2):bqab255. doi: 10.1210/endocr/bqab255.