PMID- 34919584 OWN - NLM STAT- MEDLINE DCOM- 20220110 LR - 20240404 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 16 IP - 12 DP - 2021 TI - A method for campus-wide SARS-CoV-2 surveillance at a large public university. PG - e0261230 LID - 10.1371/journal.pone.0261230 [doi] LID - e0261230 AB - The systematic screening of asymptomatic and pre-symptomatic individuals is a powerful tool for controlling community transmission of infectious disease on college campuses. Faced with a paucity of testing in the beginning of the COVID-19 pandemic, many universities developed molecular diagnostic laboratories focused on SARS-CoV-2 diagnostic testing on campus and in their broader communities. We established the UC Santa Cruz Molecular Diagnostic Lab in early April 2020 and began testing clinical samples just five weeks later. Using a clinically-validated laboratory developed test (LDT) that avoided supply chain constraints, an automated sample pooling and processing workflow, and a custom laboratory information management system (LIMS), we expanded testing from a handful of clinical samples per day to thousands per day with the testing capacity to screen our entire campus population twice per week. In this report we describe the technical, logistical, and regulatory processes that enabled our pop-up lab to scale testing and reporting capacity to thousands of tests per day. FAU - Chang, Terren AU - Chang T AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Draper, Jolene M AU - Draper JM AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Van den Bout, Anouk AU - Van den Bout A AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Kephart, Ellen AU - Kephart E AUID- ORCID: 0000-0002-3117-4439 AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Maul-Newby, Hannah AU - Maul-Newby H AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Vasquez, Yvonne AU - Vasquez Y AUID- ORCID: 0000-0001-6113-5222 AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Woodbury, Jason AU - Woodbury J AUID- ORCID: 0000-0003-4391-7276 AD - Thirdwave Analytics, San Francisco, California, United States of America. FAU - Randi, Savanna AU - Randi S AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Pedersen, Martina AU - Pedersen M AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Nave, Maeve AU - Nave M AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - La, Scott AU - La S AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Gallagher, Natalie AU - Gallagher N AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - McCabe, Molly M AU - McCabe MM AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Dhillon, Namrita AU - Dhillon N AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Bjork, Isabel AU - Bjork I AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Luttrell, Michael AU - Luttrell M AD - Business and Accounting Services, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Dang, Frank AU - Dang F AD - University of California Davis, Davis, California, United States of America. FAU - MacMillan, John B AU - MacMillan JB AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Green, Ralph AU - Green R AUID- ORCID: 0000-0002-9935-230X AD - University of California Davis, Davis, California, United States of America. FAU - Miller, Elizabeth AU - Miller E AD - Student Health Services, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Kilpatrick, Auston M AU - Kilpatrick AM AD - Department of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Vaske, Olena AU - Vaske O AUID- ORCID: 0000-0002-1677-417X AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Stone, Michael D AU - Stone MD AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America. FAU - Sanford, Jeremy R AU - Sanford JR AUID- ORCID: 0000-0002-1131-1627 AD - Colligan Clinical Diagnostic Laboratory, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Center for Molecular Biology of RNA, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America. AD - UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, California, United States of America. LA - eng GR - R01 GM095850/GM/NIGMS NIH HHS/United States GR - R35 GM130361/GM/NIGMS NIH HHS/United States GR - T32 GM133391/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20211217 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - COVID-19/*diagnosis MH - COVID-19 Nucleic Acid Testing/*methods MH - Clinical Laboratory Techniques/*methods MH - Diagnostic Screening Programs MH - Diagnostic Tests, Routine/*methods MH - Humans MH - Mass Screening/*methods MH - Pandemics/*prevention & control MH - Universities PMC - PMC8682906 COIS- MDS and JRS are paid consultants and have an ownership interest in SummerBio, a commercial COVID testing laboratory that specializes in institutional surveillance. JW is an employee of Thirdwave Analytics. This does not alter our adherence to PLOS ONE policies on sharing data and materials. EDAT- 2021/12/18 06:00 MHDA- 2022/01/11 06:00 PMCR- 2021/12/17 CRDT- 2021/12/17 17:18 PHST- 2021/07/19 00:00 [received] PHST- 2021/11/24 00:00 [accepted] PHST- 2021/12/17 17:18 [entrez] PHST- 2021/12/18 06:00 [pubmed] PHST- 2022/01/11 06:00 [medline] PHST- 2021/12/17 00:00 [pmc-release] AID - PONE-D-21-23418 [pii] AID - 10.1371/journal.pone.0261230 [doi] PST - epublish SO - PLoS One. 2021 Dec 17;16(12):e0261230. doi: 10.1371/journal.pone.0261230. eCollection 2021.