PMID- 34919801
OWN - NLM
STAT- MEDLINE
DCOM- 20220210
LR  - 20220210
IS  - 1878-1551 (Electronic)
IS  - 1534-5807 (Linking)
VI  - 57
IP  - 1
DP  - 2022 Jan 10
TI  - Targeted search for scaling genes reveals matrixmetalloproteinase 3 as a scaler 
      of the dorsal-ventral pattern in Xenopus laevis embryos.
PG  - 95-111.e12
LID - S1534-5807(21)00945-X [pii]
LID - 10.1016/j.devcel.2021.11.021 [doi]
AB  - How embryos scale patterning according to size is still not fully understood. 
      Through in silico screening and analysis of reaction-diffusion systems that could 
      be responsible for scaling, we predicted the existence of genes whose expression 
      is sensitive to embryo size and which regulate the scaling of embryonic 
      patterning. To find these scalers, we identified genes with strongly altered 
      expression in half-size Xenopus laevis embryos compared with full-size siblings 
      at the gastrula stage. Among found genes, we investigated the role of matrix 
      metalloproteinase-3 (mmp3), which was most strongly downregulated in half-size 
      embryos. We show that Mmp3 scales dorsal-ventral patterning by degrading the 
      slowly diffusing embryonic inducers Noggin1 and Noggin2 but preventing cleavage 
      of the more rapidly diffusing inducer Chordin via degradation of a Tolloid-type 
      proteinase. In addition to unraveling the mechanism underlying the scaling of 
      dorsal-ventral patterning, this work provides proof of principal for scalers 
      identification in embryos of other species.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Orlov, Eugeny E
AU  - Orlov EE
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of 
      Sciences, 117997 Moscow, Russia.
FAU - Nesterenko, Alexey M
AU  - Nesterenko AM
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of 
      Sciences, 117997 Moscow, Russia; Federal Center of Brain Research and 
      Neurotechnology, Federal Medical Biological Agency, 117997 Moscow, Russia.
FAU - Korotkova, Daria D
AU  - Korotkova DD
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of 
      Sciences, 117997 Moscow, Russia; Global Health Institute, School of Life 
      Sciences, EPFL, Lausanne, Switzerland.
FAU - Parshina, Elena A
AU  - Parshina EA
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of 
      Sciences, 117997 Moscow, Russia.
FAU - Martynova, Natalia Yu
AU  - Martynova NY
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of 
      Sciences, 117997 Moscow, Russia; Pirogov Russian National Research Medical 
      University, Moscow, Russia.
FAU - Zaraisky, Andrey G
AU  - Zaraisky AG
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of 
      Sciences, 117997 Moscow, Russia; Pirogov Russian National Research Medical 
      University, Moscow, Russia. Electronic address: azaraisky@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211216
PL  - United States
TA  - Dev Cell
JT  - Developmental cell
JID - 101120028
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (NOG2 protein, Xenopus)
RN  - 0 (Xenopus Proteins)
RN  - 148294-77-3 (noggin protein)
RN  - 93586-27-7 (chordin)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Body Patterning/*genetics/physiology
MH  - Bone Morphogenetic Proteins/metabolism
MH  - Carrier Proteins/metabolism
MH  - Cell Size
MH  - Embryo, Nonmammalian/metabolism
MH  - Gastrula/metabolism
MH  - Glycoproteins/metabolism
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Matrix Metalloproteinase 3/*metabolism/physiology
MH  - Organizers, Embryonic/*metabolism
MH  - Signal Transduction/physiology
MH  - Xenopus Proteins/metabolism
MH  - Xenopus laevis/embryology
OTO - NOTNLM
OT  - Chordin
OT  - Noggin
OT  - Tolloid
OT  - embryonic scaling
OT  - genes regulating scaling
OT  - mathematical modeling of dorsal-ventral patterning scaling
OT  - matrix metalloproteinase-3 (Mmp3)
OT  - notochord
OT  - somitic mesoderm
COIS- Declaration of interests The authors declare no competing interests.
EDAT- 2021/12/18 06:00
MHDA- 2022/02/11 06:00
CRDT- 2021/12/17 20:09
PHST- 2020/11/02 00:00 [received]
PHST- 2021/05/01 00:00 [revised]
PHST- 2021/11/19 00:00 [accepted]
PHST- 2021/12/18 06:00 [pubmed]
PHST- 2022/02/11 06:00 [medline]
PHST- 2021/12/17 20:09 [entrez]
AID - S1534-5807(21)00945-X [pii]
AID - 10.1016/j.devcel.2021.11.021 [doi]
PST - ppublish
SO  - Dev Cell. 2022 Jan 10;57(1):95-111.e12. doi: 10.1016/j.devcel.2021.11.021. Epub 
      2021 Dec 16.