PMID- 3492284
OWN - NLM
STAT- MEDLINE
DCOM- 19870226
LR  - 20190720
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 103
IP  - 1
DP  - 1986 Nov
TI  - Independent regulation of B-cell inducing factor and IL-2 production by T 
      lymphocytes, and direct and indirect promotion of immunoglobulin secretion by 
      glucocorticosteroid.
PG  - 199-206
AB  - The conditions for induction of B-cell inducing factor (BIF) by human peripheral 
      blood T cells was investigated. BIF was assayed by induction of immunoglobulin 
      secreting cells (ISC) in peripheral blood B (non-T) cells stimulated with 
      Staphylococcus aureus bacteria strain Cowan I (Sac), and in the IgM cell line 
      SKW6.4. Maximum BIF production occurred with high concentrations of the T-cell 
      mitogens phytohemagglutinin, concanavalin A, and PWM. Dexamethasone (Dex) also 
      induced BIF production in T cells at 10(-5) to 10(-7) M. At 10(-5) and 10(-6) M 
      Dex, the T-cell supernatants had to be dialyzed before testing because Dex alone 
      stimulated variable levels of ISC in both test B-cell assays. Dex did not enhance 
      BIF production by T cells that were optimally stimulated by lectin. BIF levels 
      were maximum by Day 2 of T-cell cultures and remained high at Days 3 and 4. In 
      contrast, IL-2 reached a peak at Day 1 and declined drastically by Day 4. We 
      previously showed that IL-2 at less than 100 U/ml did not induce ISC in B cells 
      and did not alter ISC induction by BIF. Dex did not induce IL-2 production and 
      inhibited IL-2 production induced by Con A, in contrast to the promoting effects 
      of Dex on BIF production, providing further evidence for the independence of BIF 
      and IL-2 production and B-cell stimulation.
FAU - Jeong, G
AU  - Jeong G
FAU - Nakoinz, I
AU  - Nakoinz I
FAU - Ralph, P
AU  - Ralph P
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Growth Substances)
RN  - 0 (Interleukin-2)
RN  - 0 (Lectins)
RN  - 0 (Lymphokines)
RN  - 207137-56-2 (Interleukin-4)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Adult
MH  - B-Lymphocytes/*metabolism
MH  - Cells, Cultured
MH  - Dexamethasone/*pharmacology
MH  - Growth Substances/*biosynthesis
MH  - Humans
MH  - Interleukin-2/*biosynthesis
MH  - Interleukin-4
MH  - Lectins/pharmacology
MH  - Lymphocyte Cooperation/drug effects
MH  - Lymphokines/*biosynthesis
MH  - T-Lymphocytes/drug effects/immunology/*metabolism
EDAT- 1986/11/01 00:00
MHDA- 1986/11/01 00:01
CRDT- 1986/11/01 00:00
PHST- 1986/11/01 00:00 [pubmed]
PHST- 1986/11/01 00:01 [medline]
PHST- 1986/11/01 00:00 [entrez]
AID - 0008-8749(86)90081-X [pii]
AID - 10.1016/0008-8749(86)90081-x [doi]
PST - ppublish
SO  - Cell Immunol. 1986 Nov;103(1):199-206. doi: 10.1016/0008-8749(86)90081-x.