PMID- 34923889
OWN - NLM
STAT- MEDLINE
DCOM- 20220204
LR  - 20220531
IS  - 1525-6006 (Electronic)
IS  - 1064-1963 (Linking)
VI  - 44
IP  - 2
DP  - 2022 Feb 17
TI  - Magnesium sulfate reduces vascular endothelial cell apoptosis in rats with 
      preeclampsia via the miR-218-5p/HMGB1 pathway.
PG  - 159-166
LID - 10.1080/10641963.2021.2013492 [doi]
AB  - OBJECTIVE: This study aims to investigate the mechanism by which magnesium 
      sulfate regulates the miR-218-5p/HMGB-pathway-mediated apoptosis of vascular 
      endothelial cells (VECs) in rats with preeclampsia (PE). METHODS: Twenty pregnant 
      rats were randomly divided into four groups: normal, PE, MgSO(4), and 
      high-mobility group protein B1 (HMGB1)-agomir groups. On the 14(th) day of each 
      rat's pregnancy, endotoxin was used to establish a PE model in the PE, MgSO(4), 
      and HMGB1-agomir groups. Then, the MgSO(4) and HMGB1-agomir groups were treated 
      with magnesium sulfate. Finally, HMGB1 overexpression was performed only in the 
      HMGB1-agomir group. The rats' urinary protein content and systolic blood pressure 
      at 24 h were detected on the 11(th), 13(th), 15(th), 17(th), and 19(th) day of 
      pregnancy. RESULTS: Compared with the PE group, 24-h urinary protein content, 
      blood pressure, VEC apoptosis rate, apoptosis marker levels, and HMGB1 expression 
      decreased while miR-218-5p levels increased in the MgSO(4) group. The 
      dual-luciferase assay revealed that HMGB1 can be targeted and regulated by 
      miR-218-5p. Compared with the MgSO(4) group, 24-h urinary protein content, blood 
      pressure, VEC apoptosis rate, apoptosis marker levels, and HMGB1 expression 
      increased while miR-218-5p levels decreased in the HMGB1-agomir group. 
      CONCLUSION: MgSO(4) reduces VEC apoptosis in PE rats via the miR-218-5p/HMGB1 
      pathway and thus plays a role in treating PE.
FAU - Zheng, Jiacui
AU  - Zheng J
AD  - Department of Obstetrics, Rizhao People's Hospital Affiliated to Jining Medical 
      Unversity, Rizhao City, Shandong, China.
FAU - Tian, Meirong
AU  - Tian M
AD  - Department of Obstetrics, Shandong Maternal and Child Health Hospital Affiliated 
      of Shandong, Jinan City, Shandong, China.
FAU - Liu, Lanlan
AU  - Liu L
AD  - Department of Obstetrics, Rizhao People's Hospital Affiliated to Jining Medical 
      Unversity, Rizhao City, Shandong, China.
FAU - Jia, Xueqin
AU  - Jia X
AD  - Department of Obstetrics, Rizhao People's Hospital Affiliated to Jining Medical 
      Unversity, Rizhao City, Shandong, China.
FAU - Sun, Meiling
AU  - Sun M
AD  - Department of Obstetrics, Rizhao People's Hospital Affiliated to Jining Medical 
      Unversity, Rizhao City, Shandong, China.
FAU - Lai, Yongjing
AU  - Lai Y
AD  - Department of Obstetrics, Rizhao People's Hospital Affiliated to Jining Medical 
      Unversity, Rizhao City, Shandong, China.
LA  - eng
PT  - Journal Article
DEP - 20211220
PL  - England
TA  - Clin Exp Hypertens
JT  - Clinical and experimental hypertension (New York, N.Y. : 1993)
JID - 9305929
RN  - 0 (HMGB1 Protein)
RN  - 0 (Hbp1 protein, rat)
RN  - 0 (MIRN218 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 7487-88-9 (Magnesium Sulfate)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Endothelial Cells/*cytology
MH  - Female
MH  - *HMGB1 Protein/genetics
MH  - Magnesium Sulfate/pharmacology
MH  - *MicroRNAs/genetics
MH  - *Pre-Eclampsia/drug therapy/genetics
MH  - Pregnancy
MH  - Rats
OTO - NOTNLM
OT  - Magnesium sulfate
OT  - high-mobility group protein b1
OT  - miR-218-5p
OT  - preeclampsia
OT  - rats
EDAT- 2021/12/21 06:00
MHDA- 2022/02/05 06:00
CRDT- 2021/12/20 05:28
PHST- 2021/12/21 06:00 [pubmed]
PHST- 2022/02/05 06:00 [medline]
PHST- 2021/12/20 05:28 [entrez]
AID - 10.1080/10641963.2021.2013492 [doi]
PST - ppublish
SO  - Clin Exp Hypertens. 2022 Feb 17;44(2):159-166. doi: 
      10.1080/10641963.2021.2013492. Epub 2021 Dec 20.