PMID- 34926655
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220429
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 9
IP  - 21
DP  - 2021 Nov
TI  - Aqueous humor proteomic analysis of acute angle-closure glaucoma with visual 
      field loss.
PG  - 1611
LID - 10.21037/atm-21-457 [doi]
LID - 1611
AB  - BACKGROUND: Acute angle-closure glaucoma (AACG) is an ophthalmic emergency that 
      occurs over the course of hours or days and may cause irreversible blindness if 
      not treated immediately. In most cases, optic nerve damage is the cause of visual 
      field (VF) loss in AACG. There has been no reliable biomarker found to evaluate 
      optic nerve damage to date. Aqueous humor (AH) proteome analysis might reveal the 
      proteomic alterations in AACG and provide helpful clues in the search for an AH 
      biomarker of optic nerve damage and VF loss. METHODS: In this study, we used the 
      AH proteome to explore the functions of differentially expressed proteins (DEPs) 
      during disease progression. The AH proteins from the early-stage group and 
      late-stage group were extracted and analyzed by the data-independent acquisition 
      (DIA) method. The DEPs functions were annotated, and parallel reaction monitoring 
      (PRM) was used to validate the key DEPs. RESULTS: A total of 87 DEPs were found. 
      Gene Ontology analysis showed that most DEPs were enriched in immunology, 
      hemodynamics, and apoptosis. Ingenuity pathway analysis found that vascular 
      endothelial growth factor (VEGF) signaling, the production of reactive oxygen 
      species (ROS) in macrophages, and the nuclear factor erythroid 2-related factor 2 
      (NRF2)-mediated oxidative stress response were active pathways in the late stage 
      of AACG. The mechanism of retinal ganglion cell (RGC) death was hypothesized on 
      the basis of DEP functional analysis. A total of 20 DEPs were validated by using 
      PRM, and prostaglandin-H2 D-isomerase was found to have the potential to evaluate 
      optic nerve damage. CONCLUSIONS: This study showed that AH proteomic analysis 
      could reveal the proteomic alterations in the pathogenesis of VF loss in AACG and 
      help to provide objective protein biomarkers to evaluate VF loss. These findings 
      will benefit the application of the AH proteome to clinical research.
CI  - 2021 Annals of Translational Medicine. All rights reserved.
FAU - Xu, Jiyu
AU  - Xu J
AD  - Core Facility of Instrument, Chinese Academy of Medical Sciences, School of Basic 
      Medicine, Peking Union Medical College, Beijing, China.
FAU - Zhao, Liangliang
AU  - Zhao L
AD  - Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, 
      China.
FAU - Liu, Xiang
AU  - Liu X
AD  - Core Facility of Instrument, Chinese Academy of Medical Sciences, School of Basic 
      Medicine, Peking Union Medical College, Beijing, China.
AD  - Shanghai AB Sciex Analytical Instrument Trading Co., Ltd Beijing Branch Company, 
      Beijing, China.
FAU - Sun, Haidan
AU  - Sun H
AD  - Core Facility of Instrument, Chinese Academy of Medical Sciences, School of Basic 
      Medicine, Peking Union Medical College, Beijing, China.
FAU - Liu, Xiaoyan
AU  - Liu X
AD  - Core Facility of Instrument, Chinese Academy of Medical Sciences, School of Basic 
      Medicine, Peking Union Medical College, Beijing, China.
FAU - Guo, Zhengguang
AU  - Guo Z
AD  - Core Facility of Instrument, Chinese Academy of Medical Sciences, School of Basic 
      Medicine, Peking Union Medical College, Beijing, China.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, 
      China.
FAU - Sun, Wei
AU  - Sun W
AD  - Core Facility of Instrument, Chinese Academy of Medical Sciences, School of Basic 
      Medicine, Peking Union Medical College, Beijing, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC8640911
OTO - NOTNLM
OT  - Aqueous humor (AH)
OT  - acute angle-closure glaucoma (AACG)
OT  - proteome
OT  - visual field (VF) loss
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at https://dx.doi.org/10.21037/atm-21-457). XL was employed by 
      Shanghai AB Sciex Analytical Instrument Trading Co., Ltd. The other authors have 
      no conflicts of interest to declare.
EDAT- 2021/12/21 06:00
MHDA- 2021/12/21 06:01
PMCR- 2021/11/01
CRDT- 2021/12/20 06:19
PHST- 2021/01/29 00:00 [received]
PHST- 2021/09/03 00:00 [accepted]
PHST- 2021/12/20 06:19 [entrez]
PHST- 2021/12/21 06:00 [pubmed]
PHST- 2021/12/21 06:01 [medline]
PHST- 2021/11/01 00:00 [pmc-release]
AID - atm-09-21-1611 [pii]
AID - 10.21037/atm-21-457 [doi]
PST - ppublish
SO  - Ann Transl Med. 2021 Nov;9(21):1611. doi: 10.21037/atm-21-457.