PMID- 34926963
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240404
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 6
IP  - 49
DP  - 2021 Dec 14
TI  - Ratiometric Fluorescent Biosensor Based on Forster Resonance Energy Transfer 
      between Carbon Dots and Acridine Orange for miRNA Analysis.
PG  - 34150-34159
LID - 10.1021/acsomega.1c05901 [doi]
AB  - The expression level of miRNA is highly correlated with the pathological process 
      of malignant tumors. Therefore, the abnormal expression of miRNA in serum is 
      considered as reliable evidence for the existence of tumor cells. Here, a 
      ratiometric fluorescent biosensor based on the Forster resonance energy transfer 
      between fluorophores is proposed for detecting colorectal cancer-specific miRNA 
      (miR-92a-3p). The miRNA in serum was first isolated by carboxyl-modified SiO(2) 
      microspheres. Then, the addition of miRNA to the detection system resulted in the 
      distance change between the donor acridine orange (AO) and the acceptor 
      fluorescent carbon dots (CDs), which made the fluorescence signal change. The 
      physicochemical properties, especially the fluorescence characteristics of CDs 
      and AO, which enabled the ratiometric fluorescence detection, were 
      comprehensively studied. The ratiometric fluorescent biosensor could detect miRNA 
      in the concentration range of 1-9 nM and showed a detection limit of 0.14 nM. 
      Moreover, the ratiometric fluorescent biosensor exhibited high selectivity for 
      the target miRNA. The validity of the ratiometric fluorescent biosensor was also 
      verified using the serum sample, demonstrating its potential for enzyme-free 
      miRNA analysis.
CI  - (c) 2021 The Authors. Published by American Chemical Society.
FAU - Sun, Zhiwei
AU  - Sun Z
AD  - Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, 
      Ministry of Education, Shandong University, Jinan 250061, China.
AD  - Shenzhen Research Institute of Shandong University, Shenzhen 518057, China.
FAU - Tong, Yao
AU  - Tong Y
AD  - Department of Clinical Laboratory, The Second Hospital, Cheeloo College of 
      Medicine, Shandong University, Jinan 250033, China.
FAU - Zhou, Xiaoyu
AU  - Zhou X
AD  - Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, 
      Ministry of Education, Shandong University, Jinan 250061, China.
FAU - Li, Juan
AU  - Li J
AD  - Department of Clinical Laboratory, The Second Hospital, Cheeloo College of 
      Medicine, Shandong University, Jinan 250033, China.
FAU - Zhao, Li
AU  - Zhao L
AD  - Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, 
      Ministry of Education, Shandong University, Jinan 250061, China.
FAU - Li, Hui
AU  - Li H
AUID- ORCID: 0000-0002-1457-8650
AD  - Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, 
      Ministry of Education, Shandong University, Jinan 250061, China.
FAU - Wang, Chuanxin
AU  - Wang C
AUID- ORCID: 0000-0002-3796-6293
AD  - Department of Clinical Laboratory, The Second Hospital, Cheeloo College of 
      Medicine, Shandong University, Jinan 250033, China.
AD  - Shandong Engineering & Technology Research Center for Tumor Marker Detection, 
      Jinan 250033, China.
AD  - Shandong Provincial Clinical Medicine Research Center for Clinical Laboratory, 
      Jinan 250033, China.
FAU - Du, Lutao
AU  - Du L
AD  - Department of Clinical Laboratory, The Second Hospital, Cheeloo College of 
      Medicine, Shandong University, Jinan 250033, China.
FAU - Jiang, Yanyan
AU  - Jiang Y
AUID- ORCID: 0000-0002-7866-4689
AD  - Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, 
      Ministry of Education, Shandong University, Jinan 250061, China.
AD  - Shenzhen Research Institute of Shandong University, Shenzhen 518057, China.
AD  - Suzhou Institute of Shandong University, Suzhou 215123, China.
LA  - eng
PT  - Journal Article
DEP - 20211130
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC8675165
COIS- The authors declare no competing financial interest.
EDAT- 2021/12/21 06:00
MHDA- 2021/12/21 06:01
PMCR- 2021/11/30
CRDT- 2021/12/20 06:24
PHST- 2021/10/21 00:00 [received]
PHST- 2021/11/22 00:00 [accepted]
PHST- 2021/12/20 06:24 [entrez]
PHST- 2021/12/21 06:00 [pubmed]
PHST- 2021/12/21 06:01 [medline]
PHST- 2021/11/30 00:00 [pmc-release]
AID - 10.1021/acsomega.1c05901 [doi]
PST - epublish
SO  - ACS Omega. 2021 Nov 30;6(49):34150-34159. doi: 10.1021/acsomega.1c05901. 
      eCollection 2021 Dec 14.